pLV3-CMV-MYH11-3×FLAG-CopGFP-P

pLV3-CMV-MYH11-3×FLAG-CopGFP-Puro plasmid

PVT95236

pLV3-CMV-MYH11-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-MYH11(human)-3×FLAG-CopGFP-Puro
Alias:
Gene lenght: 5835bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: EF1a-R
Copy number: High
Induction:

pLV3-CMV-MYH11-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-MYH11-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-MYH11-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg cgcagaaggg ccaactcagt 2341 gacgatgaga agttcctctt tgtggacaaa aacttcatca acagcccagt ggcccaggct 2401 gactgggccg ccaagagact cgtctgggtc ccctcggaga agcagggctt cgaggcagcc 2461 agcattaagg aggagaaggg ggatgaggtg gttgtggagc tggtggagaa tggcaagaag 2521 gtcacggttg ggaaagatga catccagaag atgaacccac ccaagttctc caaggtggag 2581 gacatggcgg agctgacgtg cctcaacgaa gcctccgtgc tacacaacct gagggagcgg 2641 tacttctcag ggctaatata tacgtactct ggcctcttct gcgtggtggt caacccctat 2701 aaacacctgc ccatctactc ggagaagatc gtcgacatgt acaagggcaa gaagaggcac 2761 gagatgccgc ctcacatcta cgccatcgca gacacggcct accggagcat gcttcaagat 2821 cgggaggacc agtccattct atgcacaggc gagtctggag ccgggaaaac cgaaaacacc 2881 aagaaggtca ttcagtacct ggccgtggtg gcctcctccc acaagggcaa gaaagacaca 2941 agtatcacgc aaggcccatc ttttgcctac ggagagctgg aaaagcagct tctacaagca 3001 aacccgattc tggaggcttt cggcaacgcc aaaacagtga agaacgacaa ctcctcacga 3061 ttcggcaaat tcatccgcat caacttcgac gtcacgggtt acatcgtggg agccaacatt 3121 gagacctatc tgctagaaaa atcacgggca attcgccaag ccagagacga gaggacattc 3181 cacatctttt actacatgat tgctggagcc aaggagaaga tgagaagtga cttgcttttg 3241 gagggcttca acaactacac cttcctctcc aatggctttg tgcccatccc agcagcccag 3301 gatgatgaga tgttccagga aaccgtggag gccatggcaa tcatgggttt cagcgaggag 3361 gagcagctat ccatattgaa ggtggtatca tcggtcctgc agcttggaaa tatcgtcttc 3421 aagaaggaaa gaaacacaga ccaggcgtcc atgccagata acacagctgc tcagaaagtt 3481 tgccacctca tgggaattaa tgtgacagat ttcaccagat ccatcctcac tcctcgtatc 3541 aaggttgggc gagatgtggt acagaaagct cagacaaaag aacaggctga ctttgctgta 3601 gaggctttgg ccaaggcaac atatgagcgc cttttccgct ggatactcac ccgcgtgaac 3661 aaagccctgg acaagaccca tcggcaaggg gcttccttcc tggggatcct ggatatagct 3721 ggatttgaga tctttgaggt gaactccttc gagcagctgt gcatcaacta caccaacgag 3781 aagctgcagc agctcttcaa ccacaccatg ttcatcctgg agcaggagga gtaccagcgc 3841 gagggcatcg agtggaactt catcgacttt gggctggacc tacagccctg catcgagctc 3901 atcgagcgac cgaacaaccc tccaggtgtg ctggccctgc tggacgagga atgctggttc 3961 cccaaagcca cggacaagtc tttcgtggag aagctgtgca cggagcaggg cagccacccc 4021 aagttccaga agcccaagca gctcaaggac aagactgagt tctccatcat ccattatgct 4081 gggaaggtgg actataatgc gagtgcctgg ctgaccaaga atatggaccc gctgaatgac 4141 aacgtgactt ccctgctcaa tgcctcctcc gacaagtttg tggccgacct gtggaaggac 4201 gtggaccgca tcgtgggcct ggaccagatg gccaagatga cggagagctc gctgcccagc 4261 gcctccaaga ccaagaaggg catgttccgc acagtggggc agctgtacaa ggagcagctg 4321 ggcaagctga tgaccacgct acgcaacacc acgcccaact tcgtgcgctg catcatcccc 4381 aaccacgaga agaggtccgg caagctggat gcgttcctgg tgctggagca gctgcggtgc 4441 aatggggtgc tggaaggcat tcgcatctgc cggcagggct tccccaaccg gatcgtcttc 4501 caggagttcc gccaacgcta cgagatcctg gcggcgaatg ccatccccaa aggcttcatg 4561 gacgggaagc aggcctgcat tctcatgatc aaagccctgg aacttgaccc caacttatac 4621 aggatagggc agagcaaaat cttcttccga actggcgtcc tggcccacct agaggaggag 4681 cgagatttga agatcaccga tgtcatcatg gccttccagg cgatgtgtcg tggctacttg 4741 gccagaaagg cttttgccaa gaggcagcag cagctgaccg ccatgaaggt gattcagagg 4801 aactgcgccg cctacctcaa gctgcggaac tggcagtggt ggaggctttt caccaaagtg 4861 aagccactgc tgcaggtgac acggcaggag gaggagatgc aggccaagga ggatgaactg 4921 cagaagacca aggagcggca gcagaaggca gagaatgagc ttaaggagct ggaacagaag 4981 cactcgcagc tgaccgagga gaagaacctg ctacaggaac agctgcaggc agagacagag 5041 ctgtatgcag aggctgagga gatgcgggtg cggctggcgg ccaagaagca ggagctggag 5101 gagatactgc atgagatgga ggcccgcctg gaggaggagg aagacagggg ccagcagcta 5161 caggctgaaa ggaagaagat ggcccagcag atgctggacc ttgaagaaca gctggaggag 5221 gaggaagctg ccaggcagaa gctgcaactt gagaaggtca cggctgaggc caagatcaag 5281 aaactggagg atgagatcct ggtcatggat gatcagaaca ataaactatc aaaagaacga 5341 aaactccttg aggagaggat tagtgactta acgacaaatc ttgcagaaga ggaagaaaag 5401 gccaagaatc ttaccaagct gaaaaacaag catgaatcta tgatttcaga actggaagtg 5461 cggctaaaga aggaagagaa gagccgacag gagctggaga agctgaaacg gaagctggag 5521 ggtgatgcca gcgacttcca cgagcagatc gctgacctcc aggcgcagat cgcagagctc 5581 aagatgcagc tggccaagaa ggaggaggag ctgcaggcgg ccctggccag gcttgacgat 5641 gaaatcgctc agaagaacaa tgccctgaag aagatccggg agctggaggg ccacatctca 5701 gacctccagg aggacctgga ctcagagcgg gccgccagga acaaggctga aaagcagaag 5761 cgagacctcg gcgaggagct ggaggcccta aagacagagc tggaagacac actggacagc 5821 acagccactc agcaggagct cagggccaag agggagcagg aggtgacggt gctgaagaag 5881 gccctggatg aagagacgcg gtcccatgag gctcaggtcc aggagatgag gcagaaacac 5941 gcacaggcgg tggaggagct cacagagcag cttgagcagt tcaagagggc caaggcgaac 6001 ctagacaaga ataagcagac gctggagaaa gagaacgcag acctggccgg ggagctgcgg 6061 gtcctgggcc aggccaagca ggaggtggaa cataagaaga agaagctgga ggcgcaggtg 6121 caggagctgc agtccaagtg cagcgatggg gagcgggccc gggcggagct caatgacaaa 6181 gtccacaagc tgcagaatga agttgagagc gtcacaggga tgcttaacga ggccgagggg 6241 aaggccatta agctggccaa ggacgtggcg tccctcagtt cccagctcca ggacacccag 6301 gagctgcttc aagaagaaac ccggcagaag ctcaacgtgt ctacgaagct gcgccagctg 6361 gaggaggagc ggaacagcct gcaagaccag ctggacgagg agatggaggc caagcagaac 6421 ctggagcgcc acatctccac tctcaacatc cagctctccg actcgaagaa gaagctgcag 6481 gactttgcca gcaccgtgga agctctggaa gaggggaaga agaggttcca gaaggagatc 6541 gagaacctca cccagcagta cgaggagaag gcggccgctt atgataaact ggaaaagacc 6601 aagaacaggc ttcagcagga gctggacgac ctggttgttg atttggacaa ccagcggcaa 6661 ctcgtgtcca acctggaaaa gaagcagagg aaatttgatc agttgttagc cgaggagaaa 6721 aacatctctt ccaaatacgc ggatgagagg gacagagctg aggcagaagc cagggagaag 6781 gaaaccaagg ccctgtccct ggctcgggcc cttgaagagg ccttggaagc caaagaggaa 6841 ctcgagcgga ccaacaaaat gctcaaagcc gaaatggaag acctggtcag ctccaaggat 6901 gacgtgggca agaacgtcca tgagctggag aagtccaagc gggccctgga gacccagatg 6961 gaggagatga agacgcagct ggaagagctg gaggacgagc tgcaagccac ggaggacgcc 7021 aaactgcggc tggaagtcaa catgcaggcg ctcaagggcc agttcgaaag ggatctccaa 7081 gcccgggacg agcagaatga ggagaagagg aggcaactgc agagacagct tcacgagtat 7141 gagacggaac tggaagacga gcgaaagcaa cgtgccctgg cagctgcagc aaagaagaag 7201 ctggaagggg acctgaaaga cctggagctt caggccgact ctgccatcaa ggggagggag 7261 gaagccatca agcagctacg caaactgcag gctcagatga aggactttca aagagagctg 7321 gaagatgccc gtgcctccag agatgagatc tttgccacag ccaaagagaa tgagaagaaa 7381 gccaagagct tggaagcaga cctcatgcag ctacaagagg acctcgccgc cgctgagagg 7441 gctcgcaaac aagcggacct cgagaaggag gaactggcag aggagctggc cagtagcctg 7501 tcgggaagga acgcactcca ggacgagaag cgccgcctgg aggcccggat cgcccagctg 7561 gaggaggagc tggaggagga gcagggcaac atggaggcca tgagcgaccg ggtccgcaaa 7621 gccacacagc aggccgagca gctcagcaac gagctggcca cagagcgcag cacggcccag 7681 aagaatgaga gtgcccggca gcagctcgag cggcagaaca aggagctccg gagcaagctc 7741 cacgagatgg agggggccgt caagtccaag ttcaagtcca ccatcgcggc gctggaggcc 7801 aagattgcac agctggagga gcaggtcgag caggaggcca gagagaaaca ggcggccacc 7861 aagtcgctga agcagaaaga caagaagctg aaggaaatct tgctgcaggt ggaggacgag 7921 cgcaagatgg ccgagcagta caaggagcag gcagagaaag gcaatgccag ggtcaagcag 7981 ctcaagaggc agctggagga ggcagaggag gagtcccagc gcatcaacgc caaccgcagg 8041 aagctgcagc gggagctgga tgaggccacg gagagcaacg aggccatggg ccgcgaggtg 8101 aacgcactca agagcaagct cagagggccc cccccacagg aaacttcgca gggatccGAC 8161 TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC 8221 AAGtagcggc cgcGaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca 8281 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga 8341 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag 8401 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg 8461 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc 8521 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt 8581 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc 8641 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg 8701 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc 8761 aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag cggcctgccc 8821 gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg 8881 ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa 8941 ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac 9001 ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc 9061 tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc 9121 taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc 9181 gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg 9241 caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac 9301 ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc 9361 agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc 9421 aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc 9481 agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc 9541 ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc 9601 cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc 9661 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg 9721 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc 9781 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg 9841 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc 9901 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag 9961 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg 10021 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc 10081 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc 10141 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc 10201 tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 10261 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 10321 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 10381 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 10441 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 10501 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 10561 ctgttgggca ctgacaattc cgtggtgttg tcggggaaAT CATCGTCCTT TCCTtggctg 10621 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 10681 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 10741 ctTcgccttc gccctcagac gagtcggatc tccctttggG ccgcctcccc gcctggtacc 10801 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 10861 gggactggaa gggctaattc actcccaacg aaGataagat ctgctttttg cttgtactgg 10921 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 10981 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 11041 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 11101 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga 11161 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 11221 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 11281 caatgtatct tatcatgtct ggctctagct aTCCCGCCCC TAACTCCGCC CAtcccgccc 11341 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 11401 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 11461 ggaggcctag acttttgcag agaccaaatt cgtaatcatg tcatagctgt ttcctgtgtg 11521 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 11581 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 11641 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 11701 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 11761 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 11821 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 11881 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 11941 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 12001 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 12061 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 12121 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 12181 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 12241 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 12301 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 12361 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 12421 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 12481 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 12541 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 12601 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 12661 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 12721 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 12781 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 12841 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 12901 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 12961 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 13021 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 13081 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 13141 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 13201 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 13261 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 13321 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 13381 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 13441 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 13501 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 13561 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 13621 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 13681 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga 13741 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga 13801 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg 13861 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat 13921 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg 13981 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 14041 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 14101 taaaacgacg gccagtgcca agctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.