pHAGE-STUM-3×FLAG-Puro plasmid

pHAGE-STUM-3×FLAG-Puro plasmid

PVT95106

pHAGE-STUM-3×FLAG-Puro plasmid Information

Plasmid name: pHAGE-STUM(human)-3×FLAG-Puro
Alias: NM_001003665.4;C1orf95;
Gene lenght: 423bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: PGK-R
Copy number: High
Induction:

pHAGE-STUM-3×FLAG-Puro plasmid Description

pHAGE-STUM-3×FLAG-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pHAGE-STUM-3×FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga 2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaccgtca gatcgcctgg 2761 agacgccatc cacgctgttt tgacctccat agaagacacc ggcggccacg cgtCGAAGCC 2821 CGGGCGGATC GGGTTTAAAC GGATCCGGAG GTACCGCCAC Catggagccc tcgcacaaag 2881 acgccgagac ggcggcggcg gcggcggcgg tggcggcggc ggacccccgg ggggcgtcct 2941 cgtccagcgg ggtggtggtg caggtccgcg agaagaaggg ccccctgcgc gccgccatcc 3001 cctacatgcc cttccccgtg gccgtcatct gcctcttcct caacactttc gtgccgggac 3061 tggggacatt cgtctcggcc ttcactgtgc tgtgcggggc ccgcaccgac ctcccggaca 3121 ggcatgtgtg ctgcgtcttc tggctgaaca ttgcagcagc cctcatccaa atcctcactg 3181 ccatcgtcat ggtgggctgg atcatgagca tcttctgggg catggacatg gtcatccttg 3241 ccatttccca aggctacaag gagcagggca tcccacagca gctgGATTAC AAGGATGACG 3301 ACGATAAGGA TTACAAGGAT GACGACGATA AGGATTACAA GGATGACGAC GATAAGTAGT 3361 CGAGAATTCT ACCgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta 3421 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca 3481 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact 3541 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa 3601 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag 3661 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag 3721 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc 3781 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg 3841 ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg 3901 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc 3961 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca 4021 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca 4081 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg 4141 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc 4201 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt 4261 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg 4321 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct 4381 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg 4441 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaatcgata 4501 gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 4561 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 4621 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 4681 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 4741 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 4801 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 4861 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg 4921 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 4981 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 5041 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag 5101 atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa 5161 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta 5221 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 5281 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 5341 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 5401 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat 5461 cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag 5521 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg 5581 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 5641 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 5701 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca 5761 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat 5821 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 5881 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 5941 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct 6001 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 6061 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 6121 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 6181 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 6241 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta 6301 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 6361 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 6421 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 6481 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 6541 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata 6601 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 6661 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 6721 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 6781 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 6841 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 6901 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 6961 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 7021 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 7081 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 7141 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 7201 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 7261 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 7321 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 7381 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 7441 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc 7501 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 7561 taatgcagca agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 7621 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 7681 aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 7741 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct 7801 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg 7861 attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 7921 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat 7981 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc 8041 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt 8101 actacaaact tagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.