pHAGE-3×FLAG-KHSRP-Puro plasmi

pHAGE-3×FLAG-KHSRP-Puro plasmid

PVT96473

pHAGE-3×FLAG-KHSRP-Puro plasmid Information

Plasmid name: pHAGE-3×FLAG-KHSRP(human-gc-opt)-Puro
Alias: NM_001366299.1;FBP2; FUBP2; KSRP; p75
Gene lenght: 2241bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pHAGE-3×FLAG-KHSRP-Puro plasmid Description

pHAGE-3×FLAG-KHSRP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pHAGE-3×FLAG-KHSRP-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga 2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg 2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccac gcgtGCCACC 2821 ATGgattaca aggatgacga cgataaggac tataaggacg atgatgacaa ggactacaaa 2881 gatgatgacg ataaaCGAAG CCCGGGCGGA TCGGGTTTAA ACGGATCCAT GAGCGACTAC 2941 TCTACCGGCG GACCACCACC AGGTCCACCA CCACCTGCTG GAGGCGGTGG AGGCGCTGGT 3001 GGAGCCGGTG GTGGACCTCC ACCTGGACCA CCAGGCGCAG GTGATAGAGG TGGCGGAGGT 3061 CCAGGCGGTG GCGGACCTGG AGGTGGATCT GCTGGCGGAC CAAGTCAGCC TCCAGGTGGC 3121 GGTGGACCTG GCATTCGGAA AGACGCATTT GCAGATGCTG TTCAGAGAGC TAGGCAAATC 3181 GCTGCTAAGA TCGGAGGTGA TGCTGCTACA ACCGTGAACA ACAGCACACC TGACTTCGGA 3241 TTTGGTGGTC AGAAACGACA ACTGGAAGAT GGTGATCAAC CTGAGAGCAA GAAGCTGGCT 3301 TCTCAAGGTG ACAGCATCTC TAGCCAACTT GGACCTATTC ACCCTCCACC TAGAACCAGC 3361 ATGACCGAAG AGTACAGAGT TCCAGATGGT ATGGTTGGTC TGATCATCGG CAGAGGTGGA 3421 GAACAGATCA ACAAGATCCA GCAAGACAGT GGTTGCAAAG TGCAGATCTC TCCAGATAGT 3481 GGTGGTTTGC CTGAGAGATC AGTGAGTCTG ACAGGTGCTC CAGAAAGCGT TCAGAAGGCC 3541 AAGATGATGC TCGATGACAT CGTCTCAAGA GGTCGAGGTG GTCCACCAGG TCAATTCCAC 3601 GACAACGCTA ATGGTGGACA GAATGGTACC GTACAAGAGA TCATGATACC TGCTGGTAAG 3661 GCTGGATTGG TCATCGGAAA GGGAGGTGAA ACCATCAAGC AACTGCAAGA GAGAGCTGGT 3721 GTGAAGATGA TTCTGATCCA AGACGGTTCT CAGAACACCA ACGTTGACAA GCCTCTGAGA 3781 ATCATCGGAG ATCCATACAA GGTGCAACAG GCTTGTGAGA TGGTCATGGA CATTCTGCGA 3841 GAAAGGGATC AAGGTGGATT TGGAGATCGG AATGAGTACG GTAGTCGAAT CGGAGGCGGT 3901 ATTGATGTGC CTGTACCTAG ACATAGCGTA GGTGTGGTTA TCGGCAGAAG TGGAGAGATG 3961 ATCAAGAAGA TTCAGAACGA TGCTGGTGTC AGAATCCAGT TCAAGCAAGA CGATGGAACA 4021 GGTCCAGAGA AGATCGCTCA CATCATGGGT CCACCAGACA GATGTGAGCA TGCTGCTCGG 4081 ATCATCAACG ACCTTCTGCA GTCTTTGCGG AGTGGACCAC CTGGACCTCC AGGCGGACCA 4141 GGTATGCCAC CTGGCGGTAG AGGTAGAGGC AGAGGTCAAG GTAACTGGGG TCCACCTGGT 4201 GGCGAAATGA CCTTCAGCAT TCCAACGCAC AAGTGTGGAC TTGTGATCGG AAGAGGTGGT 4261 GAGAACGTCA AGGCTATCAA CCAGCAAACA GGTGCATTTG TGGAGATCAG CAGACAGTTG 4321 CCACCTAACG GTGATCCAAA CTTCAAGCTG TTCATCATCC GAGGTTCTCC ACAACAGATC 4381 GATCATGCCA AACAGCTGAT CGAAGAGAAG ATTGAGGGTC CATTGTGTCC AGTGGGTCCA 4441 GGTCCAGGTG GACCAGGTCC AGCTGGTCCA ATGGGTCCAT TCAATCCAGG TCCATTTAAC 4501 CAGGGACCAC CTGGCGCACC ACCTCATGCT GGCGGTCCAC CTCCACATCA GTATCCACCT 4561 CAAGGTTGGG GTAACACCTA TCCACAATGG CAACCTCCAG CACCTCATGA TCCAAGCAAA 4621 GCAGCCGCAG CTGCTGCTGA TCCAAATGCT GCTTGGGCTG CTTACTACAG CCACTACTAC 4681 CAGCAACCAC CTGGTCCAGT TCCAGGACCT GCACCAGCAC CTGCTGCACC ACCAGCACAA 4741 GGTGAACCTC CACAACCTCC ACCAACTGGA CAAAGCGATT ACACCAAGGC TTGGGAAGAG 4801 TACTACAAGA AGATCGGACA ACAACCTCAA CAACCTGGTG CTCCACCTCA GCAAGACTAC 4861 ACCAAAGCAT GGGAAGAATA CTATAAGAAG CAAGCTCAAG TTGCTACCGG TGGCGGTCCA 4921 GGCGCACCTC CAGGTTCTCA ACCTGACTAC TCTGCTGCAT GGGCTGAGTA CTATCGGCAA 4981 CAAGCTGCCT ATTACGGTCA AACGCCTGGT CCAGGCGGAC CTCAACCACC ACCTACACAA 5041 CAAGGTCAAC AGCAAGCATC TGGTAACTGT CATCCACCAC CTCCACCTTT CAGCTTTCAG 5101 CCACCTGCTA CAGTTCATCC AGCATTGGTA GGTAGTGCTG GAAACCCTTT CCCTTGTGGT 5161 GTTTGTCCAt gaCTCGAGTC TAGAGGGCCC TTAGTCGAGA ATTCTACCgg gtaggggagg 5221 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct 5281 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc 5341 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc 5401 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc 5461 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc 5521 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg 5581 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg 5641 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg 5701 gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc 5761 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc 5821 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga 5881 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc 5941 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat 6001 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg 6061 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc 6121 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc 6181 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta 6241 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg 6301 gtgcatgacc cgcaagcccg gtgcctgaat cgatagatcc taatcaacct ctggattaca 6361 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 6421 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 6481 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac 6541 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca 6601 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 6661 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg 6721 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga 6781 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 6841 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga 6901 gtcggatctc cctttgggcc gcctccccgc ctgagatcct ttaagaccaa tgacttacaa 6961 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca 7021 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat 7081 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 7141 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 7201 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat 7261 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc ccgggttaat 7321 taaggaaagg gctagatcat tcttgaagac gaaagggcct cgtgatacgc ctatttttat 7381 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg 7441 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 7501 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 7561 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 7621 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 7681 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 7741 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg 7801 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 7861 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 7921 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 7981 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 8041 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 8101 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 8161 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 8221 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 8281 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 8341 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 8401 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 8461 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 8521 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 8581 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 8641 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 8701 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca 8761 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 8821 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 8881 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 8941 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 9001 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 9061 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 9121 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 9181 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 9241 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 9301 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac 9361 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcaagctc atggctgact 9421 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta 9481 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt 9541 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt 9601 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg 9661 gataacaatt tcacacagga aacagctatg acatgattac gaatttcaca aataaagcat 9721 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 9781 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta 9841 ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat 9901 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.