pLV3-CMV-MT1X-3×FLAG-Fluc-Blas

pLV3-CMV-MT1X-3×FLAG-Fluc-Blast plasmid

PVT95104

pLV3-CMV-MT1X-3×FLAG-Fluc-Blast plasmid Information

Plasmid name: pLV3-CMV-MT1X(human)-3×FLAG-Fluc-Blast
Alias: NM_005952.4;MT-1l; MT1
Gene lenght: 183bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling: Fluc
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MT1X-3×FLAG-Fluc-Blast plasmid Description

pLV3-CMV-MT1X-3×FLAG-Fluc-Blast is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-MT1X-3×FLAG-Fluc-Blast plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatggaccc caactgctcc tgctcgcctg ttggctcctg 2341 tgcctgtgcc ggctcctgca aatgcaaaga gtgcaaatgc acctcctgca agaagagctg 2401 ctgctcctgc tgccctgtgg gctgtgccaa gtgtgcccag ggctgcatct gcaaagggac 2461 gtcagacaag tgcagctgct gtgccCTCGA GGACTACAAA GACCATGACG GTGATTATAA 2521 AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGTAG ggatccgcgg ccgcGaagga 2581 tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga 2641 agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact 2701 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata 2761 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc 2821 tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc 2881 atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt 2941 ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt 3001 ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct 3061 acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta 3121 cgccaccatg gaagatgcca aaaacattaa gaagggccca gcgccattct acccactcga 3181 agacgggacc gccggcgagc agctgcacaa agccatgaag cgctacgccc tggtgcccgg 3241 caccatcgcc tttaccgacg cacatatcga ggtggacatt acctacgccg agtacttcga 3301 gatgagcgtt cggctggcag aagctatgaa gcgctatggg ctgaatacaa accatcggat 3361 cgtggtgtgc agcgagaata gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat 3421 cggtgtggct gtggccccag ctaacgacat ctacaacgag cgcgagctgc tgaacagcat 3481 gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa 3541 cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc atggatagca agaccgacta 3601 ccagggcttc caaagcatgt acaccttcgt gacttcccat ttgccacccg gcttcaacga 3661 gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga tcatgaacag 3721 tagtggcagt accggattgc ccaagggcgt agccctaccg caccgcaccg cttgtgtccg 3781 attcagtcat gcccgcgacc ccatcttcgg caaccagatc atccccgaca ccgctatcct 3841 cagcgtggtg ccatttcacc acggcttcgg catgttcacc acgctgggct acttgatctg 3901 cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc gcagcttgca 3961 agactataag attcaatctg ccctgctggt gcccacacta tttagcttct tcgctaagag 4021 cactctcatc gacaagtacg acctaagcaa cttgcacgag atcgccagcg gcggggcgcc 4081 gctcagcaag gaggtaggtg aggccgtggc caaacgcttc cacctaccag gcatccgcca 4141 gggctacggc ctgacagaaa caaccagcgc cattctgatc acccccgaag gggacgacaa 4201 gcctggcgca gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg acttggacac 4261 cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat 4321 gagcggctac gttaacaacc ccgaggctac aaacgctctc atcgacaagg acggctggct 4381 gcacagcggc gacatcgcct actgggacga ggacgagcac ttcttcatcg tggaccggct 4441 gaagagcctg atcaaataca agggctacca ggtagcccca gccgaactgg agagcatcct 4501 gctgcaacac cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg 4561 cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa accatgaccg agaaggagat 4621 cgtggactat gtggccagcc aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt 4681 cgtggacgag gtgcctaaag gactgaccgg caagttggac gcccgcaaga tccgcgagat 4741 tctcattaag gccaagaagg gcggcaagat cgccgtgtta gcggccgctg agggcagagg 4801 aagtcttcta acatgcggtg acgtggagga gaatcccggc ccttccggaA TGGCCAAGCC 4861 TTTGTCTCAA GAAGAATCCA CCCTCATTGA AAGAGCAACG GCTACAATCA ACAGCATCCC 4921 CATCTCTGAA GACTACAGCG TCGCCAGCGC AGCTCTCTCT AGCGACGGCC GCATCTTCAC 4981 TGGTGTCAAT GTATATCATT TTACTGGGGG ACCTTGTGCA GAACTCGTGG TGCTGGGCAC 5041 TGCTGCTGCT GCGGCAGCTG GCAACCTGAC TTGTATCGTC GCGATCGGAA ATGAGAACAG 5101 GGGCATCTTG AGCCCCTGCG GACGGTGCCG ACAGGTGCTT CTCGATCTGC ATCCTGGGAT 5161 CAAAGCCATA GTGAAGGACA GTGATGGACA GCCGACGGCA GTTGGGATTC GTGAATTGCT 5221 GCCCTCTGGT TATGTGTGGG AGGGCTAAgt cgacaatcaa cctctggatt acaaaatttg 5281 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 5341 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 5401 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 5461 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 5521 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 5581 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 5641 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg 5701 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 5761 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat 5821 ctccctttgg gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg 5881 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac 5941 gaaaataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct 6001 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 6061 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 6121 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt 6181 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt 6241 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 6301 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc 6361 tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 6421 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 6481 aggctttttt ggaggcctag acttttgcag agacggccca aattcgtaat catggtcata 6541 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 6601 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 6661 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 6721 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 6781 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 6841 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 6901 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 6961 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 7021 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 7081 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 7141 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 7201 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 7261 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 7321 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 7381 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 7441 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 7501 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 7561 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 7621 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 7681 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 7741 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 7801 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 7861 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 7921 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 7981 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 8041 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 8101 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 8161 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 8221 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 8281 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 8341 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 8401 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 8461 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 8521 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 8581 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 8641 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 8701 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc 8761 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 8821 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 8881 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca 8941 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc 9001 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 9061 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 9121 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.