pLV3-CMV-MED26-3×FLAG-CopGFP-P

pLV3-CMV-MED26-3×FLAG-CopGFP-Puro plasmid

PVT88458

pLV3-CMV-MED26-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-MED26(human)-3×FLAG-CopGFP-Puro
Alias: NM_004831.5;CRSP7; CRSP70
Gene lenght: 1800bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MED26-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-MED26-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-MED26-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga cagcggctcc ggcgtctccg 2341 cagcagatca gggaccggct gctgcaggcc atcgaccccc agagcaacat ccggaacatg 2401 gtggcggtgc tggaagtcat ctccagcctg gagaaatacc ctattaccaa agaggcactt 2461 gaggaaacac gacttgggaa gctcatcaac gacgtccgca agaaaaccaa gaacgaggag 2521 ctcgccaagc gggccaagaa gctgctgcgg agctggcaga agctcatcga gccggcacac 2581 cagcatgagg cggcgctgcg ggggctggcg ggggccaccg gctctgccaa cgggggcgca 2641 cacaactgcc ggccggaggt gggggcggct ggcccaccca ggagcatcca tgacctgaag 2701 agccgcaatg acctccagag gctgcccggg cagcggctgg acaggctggg cagccgcaag 2761 cgccggggtg accagcgtga cctcggccac ccagggccgc cacccaaggt ctccaaagct 2821 agccacgacc ccctggtccc caactcatcc cccctcccca ccaacgggat cagtgggagt 2881 ccagagagct tcgccagctc cctggatggc agtgggcatg caggcccaga gggcagccgc 2941 ctggagcgtg acgagaatga caagcacagt ggcaagatcc ccgtcaacgc cgtgcgaccg 3001 cacaccagct ccccgggcct gggcaagccc cctggaccct gcttgcagcc aaaggcttcg 3061 gtgctgcagc agctggacag ggtggacgag actccggggc ctccccatcc caagggaccc 3121 cctcgctgct ctttcagtcc tcggaactca cggcatgagg gctcctttgc ccggcagcag 3181 agcttgtatg cacccaaggg ctccgtgccc agcccctcac cgcggcccca ggcactcgat 3241 gccacacagg tgccgtcacc gcttccactg gcacagccgt ccacaccccc cgtacggcgg 3301 ctcgagctgc tgcccagtgc ggaaagccca gtgtgctggc ttgagcagcc tgagagccac 3361 cagcggctgg cggggccggg ctgcaaggca gggctgtccc cagccgagcc cctcctgtcc 3421 cgggcaggct tttccccaga ctcctccaag gcggacagtg atgctgcctc ctcagggggc 3481 tcggacagta aaaagaagaa gaggtaccga cctcgagact atacggttaa cttggacggg 3541 caggtggctg aggcgggcgt caagcctgtc cggttaaaag agcggaagct cacctttgac 3601 cccatgacga gacagatcaa acctctgacc cagaaagagc cagtgcgggc agacagccct 3661 gtgcacatgg agcagcagtc caggacagag ctggacaagc aggaggccaa ggccagcctc 3721 cagagcccct tcgaacagac gaactggaag gagctgtcac gcaacgagat catccagtcc 3781 tacctgagcc ggcagagcag cctgctctca tcatcgggcg cgcagacccc aggggctcac 3841 cacttcatgt ctgagtacct gaagcaggag gagagcaccc ggcaaggggc caggcagctg 3901 catgtgctgg tgcctcaaag cccgcccacg gacctccctg gtctgacccg ggaggtcaca 3961 caggacgatc tcgacagaat ccaggccagc cagtggccgg gggtgaacgg gtgtcaggac 4021 acacagggta actggtatga ctggacgcag tgcatatcgc tcgatccgca cggcgacgac 4081 gggcgcttga acattctgcc ttatgtctgc ttggacggat ccGACTACAA AGACCATGAC 4141 GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG ATGACAAGta gcggccgcGa 4201 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc 4261 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta 4321 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg 4381 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca 4441 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc 4501 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact 4561 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc 4621 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa 4681 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg 4741 cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag 4801 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc 4861 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc 4921 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc 4981 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc 5041 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc 5101 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc 5161 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat 5221 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc 5281 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac 5341 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc 5401 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag 5461 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc 5521 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac 5581 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 5641 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 5701 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 5761 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 5821 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 5881 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 5941 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 6001 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 6061 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 6121 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg 6181 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 6241 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 6301 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 6361 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 6421 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 6481 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 6541 aattccgtgg tgttgtcggg gaaATCATCG TCCTTTCCTt ggctgctcgc ctgtgttgcc 6601 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 6661 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctTcg ccttcgccct 6721 cagacgagtc ggatctccct ttggGccgcc tccccgcctg gtacctttaa gaccaatgac 6781 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct 6841 aattcactcc caacgaaGat aagatctgct ttttgcttgt actgggtctc tctggttaga 6901 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 6961 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 7021 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca 7081 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt 7141 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 7201 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 7261 tgtctggctc tagctaTCCC GCCCCTAACT CCGCCCAtcc cgcccctaac tccgcccagt 7321 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 7381 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt 7441 tgcagagacc aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct 7501 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 7561 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 7621 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 7681 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 7741 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 7801 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 7861 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 7921 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 7981 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 8041 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 8101 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 8161 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 8221 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 8281 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 8341 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 8401 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 8461 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 8521 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 8581 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 8641 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 8701 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 8761 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 8821 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 8881 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 8941 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 9001 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 9061 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 9121 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 9181 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 9241 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 9301 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 9361 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 9421 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 9481 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 9541 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 9601 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 9661 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg 9721 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 9781 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc 9841 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 9901 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg 9961 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag 10021 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag 10081 tgccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.