pCMV-FLAG-RAD50-Neo plasmid

pCMV-FLAG-RAD50-Neo plasmid

PVT95076

pCMV-FLAG-RAD50-Neo plasmid Information

Plasmid name: pCMV-FLAG-RAD50(human)-Neo
Alias: NM_005732.4;hRad50; NBSLD; RAD502
Gene lenght: 3936bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-FLAG-RAD50-Neo plasmid Description

pCMV-FLAG-RAD50-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-FLAG-RAD50-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GATGGACTAC AAGGACGATG ATGACAAGAT GTCCCGGATC GAAAAGATGA GCATTCTGGG 961 CGTGCGGAGT TTTGGAATAG AGGACAAAGA TAAGCAAATT ATCACTTTCT TCAGCCCCCT 1021 TACAATTTTG GTTGGACCCA ATGGGGCGGG AAAGACGACC ATCATTGAAT GTCTAAAATA 1081 TATTTGTACT GGAGATTTCC CTCCTGGAAC CAAAGGAAAT ACATTTGTAC ACGATCCCAA 1141 GGTTGCTCAA GAAACAGATG TGAGAGCCCA GATTCGTCTG CAATTTCGTG ATGTCAATGG 1201 AGAACTTATA GCTGTGCAAA GATCTATGGT GTGTACTCAG AAAAGCAAAA AGACAGAATT 1261 TAAAACTCTG GAAGGAGTCA TTACTAGAAC AAAGCATGGT GAAAAGGTCA GTCTGAGCTC 1321 TAAGTGTGCA GAAATTGACC GAGAAATGAT CAGTTCTCTT GGGGTTTCCA AGGCTGTGCT 1381 AAATAATGTC ATTTTCTGTC ATCAAGAAGA TTCTAATTGG CCTTTAAGTG AAGGAAAGGC 1441 TTTGAAGCAA AAGTTTGATG AGATTTTTTC AGCAACAAGA TACATTAAAG CCTTAGAAAC 1501 ACTTCGGCAG GTACGTCAGA CACAAGGTCA GAAAGTAAAA GAATATCAAA TGGAACTAAA 1561 ATATCTGAAG CAATATAAGG AAAAAGCTTG TGAGATTCGT GATCAGATTA CAAGTAAGGA 1621 AGCCCAGTTA ACATCTTCAA AGGAAATTGT CAAATCCTAT GAGAATGAAC TTGATCCATT 1681 GAAGAATCGT CTAAAAGAAA TTGAACATAA TCTCTCTAAA ATAATGAAAC TTGACAATGA 1741 AATTAAAGCC TTGGATAGCC GAAAGAAGCA AATGGAGAAA GATAATAGTG AACTGGAAGA 1801 GAAAATGGAA AAGGTTTTTC AAGGGACTGA TGAGCAACTA AATGACTTAT ATCACAATCA 1861 CCAGAGAACA GTAAGGGAGA AAGAAAGGAA ATTGGTAGAC TGTCATCGTG AACTGGAAAA 1921 ACTAAATAAA GAATCTAGGC TTCTCAATCA GGAAAAATCA GAACTGCTTG TTGAACAGGG 1981 TCGTCTACAG CTGCAAGCAG ATCGCCATCA AGAACATATC CGAGCTAGAG ATTCATTAAT 2041 TCAGTCTTTG GCAACACAGC TAGAATTGGA TGGCTTTGAG CGTGGACCAT TCAGTGAAAG 2101 ACAGATTAAA AATTTTCACA AACTTGTGAG AGAGAGACAA GAAGGGGAAG CAAAAACTGC 2161 CAACCAACTG ATGAATGACT TTGCAGAAAA AGAGACTCTG AAACAAAAAC AGATAGATGA 2221 GATAAGAGAT AAGAAAACTG GACTGGGAAG AATAATTGAG TTAAAATCAG AAATCCTAAG 2281 TAAGAAGCAG AATGAGCTGA AAAATGTGAA GTATGAATTA CAGCAGTTGG AAGGATCTTC 2341 AGACAGGATT CTTGAACTGG ACCAGGAGCT CATAAAAGCT GAACGTGAGT TAAGCAAGGC 2401 TGAGAAAAAC AGCAATGTAG AAACCTTAAA AATGGAAGTA ATAAGTCTCC AAAATGAAAA 2461 AGCAGACTTA GACAGGACCC TGCGTAAACT TGACCAGGAG ATGGAGCAGT TAAACCATCA 2521 TACAACAACA CGTACCCAAA TGGAGATGCT GACCAAAGAC AAAGCTGACA AAGATGAACA 2581 AATCAGAAAA ATAAAATCTA GGCACAGTGA TGAATTAACC TCACTGTTGG GATATTTTCC 2641 CAACAAAAAA CAGCTTGAAG ACTGGCTACA TAGTAAATCA AAAGAAATTA ATCAGACCAG 2701 GGACAGACTT GCCAAATTGA ACAAGGAACT AGCTTCATCT GAGCAGAATA AAAATCATAT 2761 AAATAATGAA CTAAAAAGAA AGGAAGAGCA GTTGTCCAGT TACGAAGACA AGCTGTTTGA 2821 TGTTTGTGGT AGCCAGGATT TTGAAAGTGA TTTAGACAGG CTTAAAGAGG AAATTGAAAA 2881 ATCATCAAAA CAGCGAGCCA TGCTGGCTGG AGCCACAGCA GTTTACTCCC AGTTCATTAC 2941 TCAGCTAACA GACGAAAACC AGTCATGTTG CCCCGTTTGT CAGAGAGTTT TTCAGACAGA 3001 GGCTGAGTTA CAAGAAGTCA TCAGTGATTT GCAGTCTAAA CTGCGACTTG CTCCAGATAA 3061 ACTCAAGTCA ACAGAATCAG AGCTAAAAAA AAAGGAAAAG CGGCGTGATG AAATGCTGGG 3121 ACTTGTGCCC ATGAGGCAAA GCATAATTGA TTTGAAGGAG AAGGAAATAC CAGAATTAAG 3181 AAACAAACTG CAGAATGTCA ATAGAGACAT ACAGCGCCTA AAGAACGACA TAGAAGAACA 3241 AGAAACACTC TTGGGTACAA TAATGCCTGA AGAAGAAAGT GCCAAAGTAT GCCTGACAGA 3301 TGTTACAATT ATGGAGAGGT TCCAGATGGA ACTTAAAGAT GTTGAAAGAA AAATTGCACA 3361 ACAAGCAGCT AAGCTACAAG GAATAGACTT AGATCGAACT GTCCAACAAG TCAACCAGGA 3421 GAAACAAGAG AAACAGCACA AGTTAGACAC AGTTTCTAGT AAGATTGAAT TGAATCGTAA 3481 GCTTATACAG GACCAGCAGG AACAGATTCA ACATCTAAAA AGTACAACAA ATGAGCTAAA 3541 ATCTGAGAAA CTTCAGATAT CCACTAATTT GCAACGTCGT CAGCAACTGG AGGAGCAGAC 3601 TGTGGAATTA TCCACTGAAG TTCAGTCTTT GTACAGAGAG ATAAAGGATG CTAAAGAGCA 3661 GGTAAGCCCT TTGGAAACAA CATTGGAAAA GTTCCAGCAA GAAAAAGAAG AATTAATCAA 3721 CAAAAAAAAT ACAAGCAACA AAATAGCACA GGATAAACTG AATGATATTA AAGAGAAGGT 3781 TAAAAATATT CATGGCTATA TGAAAGACAT TGAGAATTAT ATTCAAGATG GGAAAGACGA 3841 CTATAAGAAG CAAAAAGAAA CTGAACTTAA TAAAGTAATA GCTCAACTAA GTGAATGCGA 3901 GAAACACAAA GAAAAGATAA ATGAAGATAT GAGACTCATG AGACAAGATA TTGATACACA 3961 GAAGATACAA GAAAGGTGGC TACAAGATAA CCTTACTTTA AGAAAAAGAA ATGAGGAACT 4021 AAAAGAAGTT GAAGAAGAAA GAAAACAACA TTTGAAGGAA ATGGGTCAAA TGCAGGTTTT 4081 GCAAATGAAA AGTGAACATC AGAAGTTGGA AGAGAACATA GACAATATAA AAAGAAATCA 4141 TAATTTGGCA TTAGGGCGAC AGAAAGGTTA TGAAGAAGAA ATTATTCATT TTAAGAAAGA 4201 ACTTCGAGAA CCACAATTTC GGGATGCTGA GGAAAAGTAT AGAGAAATGA TGATTGTTAT 4261 GAGGACAACA GAACTTGTGA ACAAGGATCT GGATATTTAT TATAAGACTC TTGACCAAGC 4321 AATAATGAAA TTTCACAGTA TGAAAATGGA AGAAATCAAT AAAATTATAC GTGACCTGTG 4381 GCGAAGTACC TATCGTGGAC AAGATATTGA ATACATAGAA ATACGGTCTG ATGCCGATGA 4441 AAATGTATCA GCTTCTGATA AAAGGCGGAA TTATAACTAC CGAGTGGTGA TGCTGAAGGG 4501 AGACACAGCC TTGGATATGC GAGGACGATG CAGTGCTGGA CAAAAGGTAT TAGCCTCACT 4561 CATCATTCGC CTGGCCCTGG CTGAAACGTT CTGCCTCAAC TGTGGCATCA TTGCCTTGGA 4621 TGAGCCAACA ACAAATCTTG ACCGAGAAAA CATTGAATCT CTTGCACATG CTCTGGTTGA 4681 GATAATAAAA AGTCGCTCAC AGCAGCGTAA CTTCCAGCTT CTGGTAATCA CTCATGATGA 4741 AGATTTTGTG GAGCTTTTAG GACGTTCTGA ATATGTGGAG AAATTCTACA GGATTAAAAA 4801 GAACATCGAT CAGTGCTCAG AGATTGTGAA ATGCAGTGTT AGCTCCCTGG GATTCAATGT 4861 TCATTAGTAG AGGGCCCGTT TAAACCCGCT GATCAGCCTC GACTGTGCCT TCTAGTTGCC 4921 AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT GCCACTCCCA 4981 CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG TGTCATTCTA 5041 TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC AATAGCAGGC 5101 ATGCTGGGGA TGCGGTGGGC TCTATGGCTT CTGAGGCGGA AAGAACCAGC TGGGGCTCTA 5161 GGGGGTATCC CCACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC 5221 GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT 5281 CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG 5341 GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT 5401 CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT 5461 TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT 5521 CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT 5581 AACAAAAATT TAACGCGAAT TAATTCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC 5641 CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG 5701 GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA 5761 GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC 5821 CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC 5881 CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG 5941 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG 6001 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA 6061 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT 6121 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT 6181 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG 6241 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT 6301 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC 6361 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC 6421 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA 6481 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG 6541 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT 6601 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG 6661 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC 6721 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA 6781 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG 6841 ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC 6901 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG 6961 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT 7021 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC 7081 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG 7141 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC 7201 ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA 7261 TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT 7321 TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC 7381 TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA 7441 AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA 7501 AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG 7561 CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG 7621 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT 7681 CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT 7741 TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC 7801 TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT 7861 GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT 7921 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC 7981 TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA 8041 AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTTT TTTTGTTTGC 8101 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG 8161 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA 8221 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT 8281 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA 8341 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG 8401 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA 8461 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT 8521 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT 8581 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA 8641 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA 8701 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA 8761 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT 8821 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA 8881 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG 8941 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC 9001 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA 9061 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT 9121 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT 9181 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 9241 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 9301 GTC

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.