pLV3-CMV-UBA7-3×HA-CopGFP-Puro

pLV3-CMV-UBA7-3×HA-CopGFP-Puro plasmid

PVT95074

pLV3-CMV-UBA7-3×HA-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-UBA7(human)-3×HA-CopGFP-Puro
Alias: NM_003335.3;D8; UBA1B; UBE1L; UBE2; UBE7
Gene lenght: 3036bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-UBA7-3×HA-CopGFP-Puro plasmid Description

pLV3-CMV-UBA7-3×HA-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-UBA7-3×HA-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg atgccctgga cgcttcgaag 2341 ctactggatg aggagctgta ttcaagacag ctgtatgtgc tgggctcacc tgccatgcag 2401 aggattcagg gagccagggt cctggtgtca ggcctgcagg gcctgggggc cgaggtggcc 2461 aagaacttgg ttctgatggg tgtgggcagc ctcactctgc atgatcccca ccccacctgc 2521 tggtccgacc tggctgccca gtttctcctc tcagagcagg acttggaaag gagcagagcc 2581 gaggcctctc aagagctctt ggctcagctc aacagagctg tccaggtcgt cgtgcacacg 2641 ggtgacatca ctgaggacct gctgttggac ttccaggtgg tggtgctgac tgctgcaaag 2701 ctggaggagc agctgaaggt gggcaccttg tgtcataagc atggagtttg ctttctggcg 2761 gctgacaccc ggggcctcgt ggggcagttg ttctgtgact ttggtgagga cttcactgtg 2821 caggacccca cagaggcaga acccctgaca gctgccatcc agcacatctc ccagggctcc 2881 cctggcattc tcactctgag gaaaggggcc aatacccact acttccgtga tggagacttg 2941 gtgactttct cgggaattga gggaatggtt gagctcaacg actgtgatcc ccggtctatc 3001 cacgtgcggg aggatgggtc cctggagatt ggagacacaa caactttctc tcggtacttg 3061 cgtggtgggg ctatcactga agtcaagaga cccaagactg tgagacataa gtccctggac 3121 acagccctgc tccagcccca tgtggtggcc cagagctccc aggaagttca ccatgcccac 3181 tgcctgcatc aggccttctg tgcactgcac aagttccagc acctccatgg ccggccaccc 3241 cagccctggg atcctgttga tgcagagact gtggtgggcc tggcccggga cctggaacca 3301 ctgaagcgga cagaggaaga gccactggaa gagccactgg atgaggccct agtgcggaca 3361 gtcgccctaa gcagtgcagg tgtcttgagc cctatggtgg ccatgctggg tgcagtagct 3421 gcccaggaag tgctgaaggc aatctccagg aagttcatgc ctctggacca gtggctttac 3481 tttgatgccc tcgattgtct tccggaagat ggggagctcc ttcccagtcc tgaggactgt 3541 gccctgagag gcagccgcta tgatgggcaa attgcagtgt ttggggctgg ttttcaggag 3601 aaactgagac gccagcacta cctcctggtg ggcgctggtg ccattggttg tgagctgctc 3661 aaagtctttg ccctagtggg actgggggcc gggaacagcg ggggcttgac tgttgttgac 3721 atggaccaca tagagcgctc caatctcagc cgtcagttcc tcttcaggtc ccaggacgtt 3781 ggtagaccca aggcagaggt ggctgcagca gctgcccggg gcctgaaccc agacttacag 3841 gtgatcccgc tcacctaccc actggatccc accacagagc acatctatgg ggataacttt 3901 ttctcccgtg tggatggtgt ggctgctgcc ctggacagtt tccaggcccg gcgctatgtg 3961 gctgctcgtt gcacccacta tctgaagcca ctgctggagg caggcacatc gggcacctgg 4021 ggcagtgcta cagtattcat gccacatgtg actgaggcct acagagcccc tgcctcagct 4081 gcagcttctg aggatgcccc ctaccctgtc tgtaccgtgc ggtacttccc tagcacagcc 4141 gagcacaccc tgcagtgggc ccggcatgag tttgaagaac tcttccgact gtctgcagag 4201 accatcaacc accaccaaca ggcacacact tccctggcag acatggatga gccacagaca 4261 ctcaccttac tgaagccagt gcttggggtc ctgagagtgc gtccacagaa ctggcaagac 4321 tgtgtggcgt gggctcttgg ccactggaaa ctctgctttc attatggcat caaacagctg 4381 ctgaggcact tcccacctaa taaagtgctt gaggatggaa ctcccttctg gtcaggtccc 4441 aaacagtgtc cccagccctt ggagtttgac accaaccaag acacacacct cctctacgta 4501 ctggcagctg ccaacctgta tgcccagatg catgggctgc ctggctcaca ggactggact 4561 gcactcaggg agctgctgaa gctgctgcca cagcctgacc cccaacagat ggcccccatc 4621 tttgctagta atctagagct ggcttcggct tctgctgagt ttggccctga gcagcagaag 4681 gaactgaaca aagccctgga agtctggagt gtgggccctc ccctgaagcc tctgatgttt 4741 gagaaggatg atgacagcaa cttccatgtg gactttgtgg tagcggcagc tagcctgaga 4801 tgtcagaact acgggattcc accggtcaac cgtgcccaga gcaagcgaat tgtgggccag 4861 attatcccag ccattgccac cactacagca gctgtggcag gcctgttggg cctggagctg 4921 tataaggtgg tgagtgggcc acggcctcgt agtgcctttc gccacagcta cctacatctg 4981 gctgaaaact acctcatccg ctatatgcct tttgccccag ccatccagac gttccatcac 5041 ctgaagtgga cctcttggga ccgtctgaag gtaccagctg ggcagcctga gaggaccctg 5101 gagtcgctgc tggctcatct tcaggagcag cacgggttga gggtgaggat cctgctgcac 5161 ggctcagccc tgctctatgc ggccggatgg tcacctgaaa agcaggccca gcacctgccc 5221 ctcagggtga cagaactggt tcagcagctg acaggccagg cacctgctcc tgggcagcgg 5281 gtgttggtgc tagagctgag ctgtgagggt gacgacgagg acactgcctt cccacctctg 5341 cactatgagc tgCTCGAGTA CCCATACGAC GTACCTGATT ATGCAACTTA TCCTTATGAT 5401 GTACCTGATT ATGCTAGCTA TCCTTATGAT GTCCCAGACT ACGCATAGgg atccCTGCGA 5461 TATCCAGCgc ggccgcGaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc 5521 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag 5581 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc 5641 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac 5701 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc 5761 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc 5821 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg 5881 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc 5941 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga 6001 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg 6061 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg 6121 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc 6181 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac 6241 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc 6301 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc 6361 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc 6421 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac 6481 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc 6541 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac 6601 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac 6661 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc 6721 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc 6781 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc 6841 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg 6901 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat 6961 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg 7021 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg 7081 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg 7141 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc 7201 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt 7261 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc 7321 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc 7381 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt 7441 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 7501 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 7561 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 7621 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 7681 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 7741 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 7801 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg 7861 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 7921 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 7981 cgtctccgcc ttcgccctca gacgagtcgg atctcccttt ggccgcctcc ccgcctggta 8041 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag 8101 gggggactgg aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact 8161 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 8221 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 8281 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 8341 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca 8401 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 8461 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 8521 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg 8581 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 8641 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca 8701 gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 8761 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 8821 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 8881 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 8941 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 9001 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 9061 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 9121 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 9181 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 9241 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 9301 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 9361 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 9421 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 9481 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 9541 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 9601 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 9661 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 9721 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 9781 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 9841 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 9901 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 9961 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 10021 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 10081 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 10141 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 10201 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 10261 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 10321 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 10381 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 10441 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 10501 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 10561 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 10621 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 10681 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 10741 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 10801 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 10861 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa 10921 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct 10981 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 11041 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg 11101 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg 11161 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag 11221 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 11281 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 11341 gtgccaagct g

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.