pLV2-CMV-HNRNPLL-3×HA-Puro pla

pLV2-CMV-HNRNPLL-3×HA-Puro plasmid

PVT91633

pLV2-CMV-HNRNPLL-3×HA-Puro plasmid Information

Plasmid name: pLV2-CMV-HNRNPLL(human)-3×HA-Puro
Alias: NM_138394.4;HNRPLL; SRRF
Gene lenght: 1626bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-HNRNPLL-3×HA-Puro plasmid Description

pLV2-CMV-HNRNPLL-3×HA-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-HNRNPLL-3×HA-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatgtcctc ctcctcttcc tcccccaggg agacgtacga 2881 ggaggaccgg gagtacgaga gccaggccaa gcgtctcaag accgaggagg gggagatcga 2941 ctactcggcc gaggaaggcg agaaccgccg ggaagcgacg ccccggggcg ggggcgatgg 3001 cggcggcggc ggccggagct tctctcagcc ggaggcaggt ggaagtcatc ataaagtttc 3061 tgtttcaccc gtcgtccatg ttcgaggact ctgtgaatct gtggtggaag cagacctcgt 3121 ggaagcgctg gaaaaatttg ggacaatatg ctatgtgatg atgatgccat ttaaacgaca 3181 ggctctagtg gaatttgaaa acatagatag tgccaaagaa tgtgtgacat ttgctgcaga 3241 tgaacccgtg tacattgctg gtcaacaggc ttttttcaac tattctacaa gcaaaaggat 3301 cactcggcca ggaaatactg atgatccatc aggaggcaac aaagttcttc tgctctcaat 3361 tcagaatccg ctttatccaa ttacagtgga tgttttatat actgtatgca accctgttgg 3421 caaagtgcaa cgtattgtta tattcaagag aaatgggata caagcaatgg ttgagtttga 3481 atcagtcctt tgtgcccaga aagctaaagc agcactcaat ggagctgata tatatgctgg 3541 atgttgcaca ctaaaaattg aatatgcacg gccaactcgt ctaaatgtta ttaggaatga 3601 caatgacagt tgggactaca ctaaaccata tttgggaaga cgagatagag gaaagggtcg 3661 ccagagacaa gccattttgg gagaacaccc ttcttcgttt agacatgatg gctatggatc 3721 ccatggtcca ttattgcctt taccaagtcg ttacagaatg ggctctcgag atacacctga 3781 acttgttgct tatccattac cacaggcttc ttcctcttac atgcatggag gaaatccctc 3841 tggttcagtt gtaatggtta gtggattaca tcaactaaaa atgaattgtt caagagtctt 3901 caacctgttc tgcttatatg gaaatattga gaaggtaaaa tttatgaaga ccattcctgg 3961 tacagcactg gtagaaatgg gtgatgagta tgctgtagaa agagctgtca cacaccttaa 4021 taatgtcaaa ttatttggga aaagacttaa tgtttgcgtg tctaaacaac attcagttgt 4081 tccaagtcaa atatttgagc tggaggatgg taccagcagc tacaaagatt ttgcaatgag 4141 caaaaataat cgctttacaa gtgctggcca agcatctaag aatataatcc agccaccctc 4201 ctgtgttttg cattattata atgttccatt gtgtgtcaca gaagagacct tcacaaagtt 4261 gtgtaatgac catgaagttc ttacattcat caaatataaa gtgtttgatg caaaaccttc 4321 agccaaaaca ctttctgggc tattagaatg ggagtgcaaa actgatgcag tagaagccct 4381 tacggcactg aatcactatc agataagagt gccgaatggt tccaatccct atacattgaa 4441 gctttgcttt tctacatcat cccatttagg atccTACCCA TACGACGTAC CTGATTATGC 4501 AACTTATCCT TATGATGTAC CTGATTATGC TAGCTATCCT TATGATGTCC CAGACTACGC 4561 ATAGtctaga taattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg 4621 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca 4681 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc 4741 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac 4801 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca 4861 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg 4921 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg 4981 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc 5041 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc 5101 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 5161 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 5221 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 5281 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 5341 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 5401 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 5461 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 5521 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 5581 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 5641 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 5701 ccgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 5761 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 5821 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 5881 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 5941 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 6001 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 6061 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt 6121 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 6181 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 6241 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc 6301 gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat 6361 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt 6421 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt 6481 agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa 6541 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac 6601 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca 6661 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct 6721 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc 6781 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga 6841 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc 6901 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac 6961 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 7021 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 7081 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 7141 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 7201 agagagtgag aggccttgac attgctagcg ttttaccgtc gacctctagc tagagcttgg 7261 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 7321 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 7381 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 7441 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 7501 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 7561 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 7621 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 7681 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 7741 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 7801 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 7861 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 7921 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 7981 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 8041 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 8101 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 8161 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 8221 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 8281 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 8341 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 8401 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 8461 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 8521 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 8581 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 8641 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 8701 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 8761 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 8821 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 8881 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 8941 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 9001 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 9061 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 9121 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 9181 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 9241 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 9301 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 9361 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 9421 ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa 9481 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 9541 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac 9601 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt 9661 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt 9721 taaatatgta ctacaaactt agtagttttt aaagaaattg tatttgttaa atatgtacta 9781 caaacttagt agt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.