pLV3-CMV-FOXA3-3×FLAG-CopGFP-P

pLV3-CMV-FOXA3-3×FLAG-CopGFP-Puro plasmid

PVT95069

pLV3-CMV-FOXA3-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-FOXA3(human)-3×FLAG-CopGFP-Puro
Alias: NM_004497.3;FKHH3; HNF3G; TCF3G
Gene lenght: 1050bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-FOXA3-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-FOXA3-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-FOXA3-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgc tgggctcagt gaagatggag 2341 gcccatgacc tggccgagtg gagctactac ccggaggcgg gcgaggtcta ctcgccggtg 2401 accccagtgc ccaccatggc ccccctcaac tcctacatga ccctgaatcc tctaagctct 2461 ccctatcccc ctggggggct ccctgcctcc ccactgccct caggacccct ggcaccccca 2521 gcacctgcag cccccctggg gcccactttc ccaggcctgg gtgtcagcgg tggcagcagc 2581 agctccgggt acggggcccc gggtcctggg ctggtgcacg ggaaggagat gccgaagggg 2641 tatcggcggc ccctggcaca cgccaagcca ccgtattcct atatctcact catcaccatg 2701 gccatccagc aggcgccggg caagatgctg accttgagtg aaatctacca gtggatcatg 2761 gacctcttcc cttactaccg ggagaatcag cagcgctggc agaactccat tcgccactcg 2821 ctgtctttca acgactgctt cgtcaaggtg gcgcgttccc cagacaagcc tggcaagggc 2881 tcctactggg ccctacaccc cagctcaggg aacatgtttg agaatggctg ctacctgcgc 2941 cgccagaaac gcttcaagct ggaggagaag gtgaaaaaag ggggcagcgg ggctgccacc 3001 accaccagga acgggacagg gtctgctgcc tcgaccacca cccccgcggc cacagtcacc 3061 tccccgcccc agcccccgcc tccagcccct gagcctgagg cccagggcgg ggaagatgtg 3121 ggggctctgg actgtggctc acccgcttcc tccacaccct atttcactgg cctggagctc 3181 ccaggggagc tgaagctgga cgcgccctac aacttcaacc accctttctc catcaacaac 3241 ctaatgtcag aacagacacc agcacctccc aaactggacg tggggtttgg gggctacggg 3301 gctgaaggtg gggagcctgg agtctactac cagggcctct attcccgctc tttgcttaat 3361 gcatccggat ccGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC 3421 AAGGATGACG ATGACAAGta gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag 3481 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga 3541 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc 3601 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt 3661 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct 3721 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg 3781 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc 3841 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc 3901 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg 3961 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac 4021 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg 4081 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag 4141 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc 4201 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc 4261 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg 4321 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg 4381 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc 4441 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc 4501 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag 4561 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg 4621 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc 4681 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc 4741 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg 4801 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 4861 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 4921 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 4981 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 5041 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 5101 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 5161 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 5221 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 5281 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 5341 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 5401 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 5461 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 5521 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 5581 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 5641 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 5701 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 5761 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaATCATCG 5821 TCCTTTCCTt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 5881 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 5941 cggcctcttc cgcgtctTcg ccttcgccct cagacgagtc ggatctccct ttggGccgcc 6001 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 6061 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaGat aagatctgct 6121 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 6181 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 6241 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 6301 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 6361 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata 6421 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 6481 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctaTCCC GCCCCTAACT 6541 CCGCCCAtcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 6601 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 6661 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata 6721 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 6781 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 6841 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 6901 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 6961 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 7021 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 7081 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 7141 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 7201 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 7261 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 7321 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 7381 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 7441 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 7501 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 7561 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 7621 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 7681 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 7741 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 7801 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 7861 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 7921 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 7981 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 8041 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 8101 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 8161 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 8221 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 8281 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 8341 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 8401 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 8461 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 8521 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 8581 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 8641 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 8701 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 8761 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 8821 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 8881 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc 8941 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 9001 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 9061 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca 9121 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc 9181 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 9241 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 9301 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.