pLV3-CMV-TK1-3×FLAG-CopGFP-Pur

pLV3-CMV-TK1-3×FLAG-CopGFP-Puro plasmid

PVT94856

pLV3-CMV-TK1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-TK1(human)-3×FLAG-CopGFP-Puro
Alias: NM_003258.5;TK2;
Gene lenght: 702bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-TK1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-TK1-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-TK1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga gctgcattaa cctgcccact 2341 gtgctgcctg gctcccccag caagacccgg gggcagatcc aggtgattct cgggccgatg 2401 ttctcaggaa aaagcacaga gttgatgaga cgcgtccgtc gcttccagat tgctcagtac 2461 aagtgcctgg tgatcaagta tgccaaagac actcgctaca gcagcagctt ctgcacacat 2521 gaccggaaca ccatggaggc actgcccgcc tgcctgctcc gagacgtggc ccaggaggcc 2581 ctgggcgtgg ctgtcatagg catcgacgag gggcagtttt tccctgacat cgtggagttc 2641 tgcgaggcca tggccaacgc cgggaagacc gtaattgtgg ctgcactgga tgggaccttc 2701 cagaggaagc catttggggc catcctgaac ctggtgccgc tggccgagag cgtggtgaag 2761 ctgacggcgg tgtgcatgga gtgcttccgg gaagccgcct ataccaagag gctcggcaca 2821 gagaaggagg tcgaggtgat tgggggagca gacaagtacc actccgtgtg tcggctctgc 2881 tacttcaaga aggcctcagg ccagcctgcc gggccggaca acaaagagaa ctgcccagtg 2941 ccaggaaagc caggggaagc cgtggctgcc aggaagctct ttgccccaca gcagattctg 3001 caatgcagcc ctgccaacgg atccGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT 3061 GACATCGACT ACAAGGATGA CGATGACAAG tagcggccgc Gaaggatctg cgatcgctcc 3121 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 3181 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 3241 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 3301 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 3361 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 3421 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 3481 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 3541 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 3601 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc 3661 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc 3721 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc 3781 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc 3841 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc 3901 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac 3961 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac 4021 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc 4081 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc 4141 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac 4201 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc 4261 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac 4321 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg 4381 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca 4441 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc 4501 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 4561 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 4621 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 4681 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 4741 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 4801 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 4861 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 4921 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 4981 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 5041 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa 5101 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 5161 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 5221 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 5281 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 5341 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 5401 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 5461 gggaaATCAT CGTCCTTTCC Ttggctgctc gcctgtgttg ccacctggat tctgcgcggg 5521 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 5581 ctgccggctc tgcggcctct tccgcgtctT cgccttcgcc ctcagacgag tcggatctcc 5641 ctttggGccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga 5701 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaG 5761 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 5821 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 5881 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 5941 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 6001 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa 6061 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 6121 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctaTC 6181 CCGCCCCTAA CTCCGCCCAt cccgccccta actccgccca gttccgccca ttctccgccc 6241 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 6301 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt 6361 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 6421 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 6481 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 6541 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 6601 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 6661 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 6721 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 6781 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 6841 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 6901 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 6961 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 7021 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 7081 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 7141 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 7201 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 7261 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 7321 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 7381 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 7441 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 7501 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 7561 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 7621 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 7681 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 7741 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 7801 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 7861 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 7921 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 7981 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 8041 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 8101 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 8161 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 8221 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 8281 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 8341 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 8401 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 8461 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 8521 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 8581 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 8641 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 8701 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 8761 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 8821 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 8881 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 8941 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.