pLV3-EF1a-Cluap1-T2A-mCherry p

pLV3-EF1a-Cluap1-T2A-mCherry plasmid

PVT94850

pLV3-EF1a-Cluap1-T2A-mCherry plasmid Information

Plasmid name: pLV3-EF1a-Cluap1(mouse)-T2A-mCherry
Alias: NM_029738.3 ;2310030D15Rik; 2610111M03Rik
Gene lenght: 1239bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Red
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: mCherry-R
Copy number: High
Induction:

pLV3-EF1a-Cluap1-T2A-mCherry plasmid Description

pLV3-EF1a-Cluap1-T2A-mCherry is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-EF1a-Cluap1-T2A-mCherry plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat 1981 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa 2041 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg 2101 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt 2161 ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc 2221 gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg 2281 gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc 2341 tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga 2401 ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca 2461 agctgtgacc ggcgcctact ctagagctag cgaattcGCC ACCatgtctt tccgcgacct 2521 ccgcaatttc accgagatga tgagagcctt ggggtaccca cgccatattt ctatggagaa 2581 tttccgcaca cccaactttg ggcttgtctc tgaagtgctt ctgtggcttg tgaaaaggta 2641 tgagccgcag actgacatcc cttctgacat tgagactgaa caagacagag ttttcttcat 2701 taaggcaatt gcccagttca tggccacaaa ggcacatata aaactcaata ccaagaagct 2761 ctatcaagct gacgggtatg cagtgaaaga gctgctgaag atcacatctg ttctctataa 2821 tgcaatgaag accaaaggga tggagggctc caatgtagga gaggaagaca tcagcaaatt 2881 caagtttgat ctcggctcca agattgcaga tttgaaagca gccagacagc ttgcgtctga 2941 aatcactgcc aaaggagcct ctctgtatga cttgctgggc aaagaggttg agctgaggga 3001 actgaggaca gaagccattg ctagacctct agagatcaat gagactgaaa aggtgatgag 3061 gattgcaatc aaagatcttt tggcccaggt tcagaagact aaagacctgc ttaataatgt 3121 ggcctctgat gaagcaaact tagaagccaa aattgaaaag agaaaattag aactggaaag 3181 aaatcggaag cgactgcaga ccctgcagag tgtcaggccg gcctttatgg atgaatatga 3241 gaaggttgag gaagacctgc agaagcagta tgacgtctat ctagagaagt tccggaacct 3301 ggcttacctg gagcagcagc ttgaggatca ccacaggatg gagcaggaga ggtttgaaga 3361 agctgaaaat actctccgtc tgatgcaaaa taagttaaag gaagaagaga agcgactgct 3421 caagagtgga agcaatgatg actcagacat agacatccaa gaggatgatg aatcagacag 3481 cgagttggag gacagacgga tgtctaagcc tcggacagcg atggaggtgc tcatgcaagg 3541 aaggcccagc aaacggatcg tgggtgcaat gcaaggtgga gactcggatg aagacgaaga 3601 ctcggaggac agtgaaattg acatggaaga tgatgaagaa gatgatgacg atttggaaga 3661 tgaaagcatt gctctctcac cagctaagcc cagtcgaagg atccgtaaac cggagccctt 3721 ggatgaaagt gacaatgact tcggatccgc ggccgctgag ggcagaggaa gtcttctaac 3781 atgcggtgac gtggaggaga atcccggccc ttccggaatg gagagcgacg agagcggcct 3841 ggtgagcaag ggcgaggagg ataacatggc gatcatcaag gagttcatgc gcttcaaggt 3901 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg 3961 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt 4021 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc 4081 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt 4141 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg 4201 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat 4261 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc 4321 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga 4381 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa 4441 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg 4501 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtagg tcgacaatca 4561 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 4621 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 4681 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 4741 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 4801 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 4861 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 4921 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg 4981 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 5041 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 5101 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac 5161 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg 5221 aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact gggtctctct 5281 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 5341 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 5401 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt 5461 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga 5521 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 5581 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 5641 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc 5701 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 5761 gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca gagacggccc 5821 aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 5881 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 5941 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 6001 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 6061 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 6121 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 6181 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 6241 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 6301 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 6361 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 6421 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 6481 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 6541 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 6601 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 6661 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 6721 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 6781 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 6841 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 6901 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 6961 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 7021 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 7081 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 7141 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 7201 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 7261 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 7321 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 7381 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 7441 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 7501 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 7561 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 7621 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 7681 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 7741 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 7801 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 7861 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 7921 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 7981 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 8041 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 8101 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 8161 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca 8221 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa 8281 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt 8341 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag 8401 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct 8461 g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.