pLV3-CMV-SEMA3D-3×Myc-Puro pla

pLV3-CMV-SEMA3D-3×Myc-Puro plasmid

PVT98764

pLV3-CMV-SEMA3D-3×Myc-Puro plasmid Information

Plasmid name: pLV3-CMV-SEMA3D(human)-3×Myc-Puro
Alias: NM_001384900.1;coll-2; Sema-Z2
Gene lenght: 2331bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SEMA3D-3×Myc-Puro plasmid Description

pLV3-CMV-SEMA3D-3×Myc-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-SEMA3D-3×Myc-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catgaatgct aataaagatg aaagacttaa agccagaagc 2341 caagattttc acctttttcc tgctttgatg atgctaagca tgaccatgtt gtttcttcca 2401 gtcactggca ctttgaagca aaatattcca agactcaagc taacctacaa agacttgctg 2461 ctttcaaata gctgtattcc ctttttgggt tcatcagaag gactggattt tcaaactctt 2521 ctcttagatg aggaaagagg caggctgctc ttgggagcca aagaccacat ctttctactc 2581 agtctggttg acttaaacaa aaattttaag aagatttatt ggcctgctgc aaaggaacgg 2641 gtggaattat gtaaattagc tgggaaagat gccaatacag aatgtgcaaa tttcatcaga 2701 gtacttcagc cctataacaa aactcacata tatgtgtgtg gaactggagc atttcatcca 2761 atatgtgggt atattgatct tggagtctac aaggaggata ttatattcaa actagacaca 2821 cataatttgg agtctggcag actgaaatgt cctttcgatc ctcagcagcc ttttgcttca 2881 gtaatgacag atgagtacct ctactctgga acagcttctg atttccttgg caaagatact 2941 gcattcactc gatcccttgg gcctactcat gaccaccact acatcagaac tgacatttca 3001 gagcactact ggctcaatgg agcaaaattt attggaactt tcttcatacc agacacctac 3061 aatccagatg atgataaaat atatttcttc tttcgtgaat catctcaaga aggcagtacc 3121 tccgataaaa ccatcctttc tcgagttgga agagtttgta agaatgatgt aggaggacaa 3181 cgcagcctga taaacaagtg gacgactttt cttaaggcca gactgatttg ctcaattcct 3241 ggaagtgatg gggcagatac ttactttgat gagcttcaag atatttattt actccccaca 3301 agagatgaaa gaaatcctgt agtatatgga gtctttacta caaccagctc catcttcaaa 3361 ggctctgctg tttgtgtgta tagcatggct gacatcagag cagtttttaa tggtccatat 3421 gctcataagg aaagtgcaga ccatcgttgg gtgcagtatg atgggagaat tccttatcca 3481 cggcctggta catgtccaag caaaacctat gacccactga ttaagtccac ccgagatttt 3541 ccagatgatg tcatcagttt cataaagcgg cactctgtga tgtataagtc cgtataccca 3601 gttgcaggag gaccaacgtt caagagaatc aatgtggatt acagactgac acagatagtg 3661 gtggatcatg tcattgcaga agatggccag tacgatgtaa tgtttcttgg aacagacatt 3721 ggaactgtcc tcaaagttgt cagcatttca aaggaaaagt ggaatatgga agaggtagtg 3781 ctggaggagt tgcagatatt caagcactca tcaatcatct tgaacatgga attgtctctg 3841 aagcagcaac aattgtacat tggttcccga gatggattgg ttcagctctc cttgcacaga 3901 tgcgacactt atgggaaagc ttgcgcagac tgttgtcttg ccagagaccc ctactgtgcc 3961 tgggatggaa atgcatgctc tcgatatgct cctacttcta aaaggagagc tagacgccaa 4021 gatgtaaaat atggcgaccc aatcacccag tgctgggaca tcgaagacag cattagtcat 4081 gaaactgctg atgaaaaggt gatttttggc attgaattta actcaacctt tctggaatgt 4141 atacctaaat cccaacaagc aactattaaa tggtatatcc agaggtcagg ggatgagcat 4201 cgagaggagt tgaagcccga tgaaagaatc atcaaaacgg aatatgggct actgattcga 4261 agtttgcaga agaaggattc tgggatgtat tactgcaaag cccaggagca cactttcatc 4321 cacaccatag tgaagctgac tttgaatgtc attgagaatg aacagatgga aaatacccag 4381 agggcagagc atgaggaggg gaaggtcaag gatctattgg ctgagtcacg gttgagatac 4441 aaagactaca tccaaatcct tagcagccca aacttcagcc tcgaccagta ctgcgaacag 4501 atgtggcaca gggagaagcg gagacagaga aacaaggggg gcccaaagtg gaagcacatg 4561 caggaaatga agaagaaacg aaatcgaaga catcacagag acctggatga gctccctaga 4621 gctgtagcca cgggatccGA ACAAAAACTA ATATCAGAGG AAGATTTGGA ACAGAAACTA 4681 ATCAGTGAAG AAGATTTAGA GCAAAAGTTA ATTTCCGAGG AGGACTTATA GCGGCCGCGA 4741 AGGATCTGCG ATCGCTCCGG TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC 4801 GAGAAGTTGG GGGGAGGGGT CGGCAATTGA ACGGGTGCCT AGAGAAGGTG GCGCGGGGTA 4861 AACTGGGAAA GTGATGTCGT GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG 4921 TATATAAGTG CAGTAGTCGC CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA 4981 CAGCTGAAGC TTCGAGGGGC TCGCATCTCT CCTTCACGCG CCCGCCGCCC TACCTGAGGC 5041 CGCCATCCAC GCCGGTTGAG TCGCGTTCTG CCGCCTCCCG CCTGTGGTGC CTCCTGAACT 5101 GCGTCCGCCG TCTAGGTAAG TTTAAAGCTC AGGTCGAGAC CGGGCCTTTG TCCGGCGCTC 5161 CCTTGGAGCC TACCTAGACT CAGCCGGCTC TCCACGCTTT GCCTGACCCT GCTTGCTCAA 5221 CTCTACGTCT TTGTTTCGTT TTCTGTTCTG CGCCGTTACA GATCCAAGCT GTGACCGGCG 5281 CCTACGCTAG ATGACCGAGT ACAAGCCCAC GGTGCGCCTC GCCACCCGCG ACGACGTCCC 5341 CCGGGCCGTA CGCACCCTCG CCGCCGCGTT CGCCGACTAC CCCGCCACGC GCCACACCGT 5401 CGACCCGGAC CGCCACATCG AGCGGGTCAC CGAGCTGCAA GAACTCTTCC TCACGCGCGT 5461 CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC GGACGACGGC GCCGCGGTGG CGGTCTGGAC 5521 CACGCCGGAG AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG ATCGGCCCGC GCATGGCCGA 5581 GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA ACAGATGGAA GGCCTCCTGG CGCCGCACCG 5641 GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC CGTCGGCGTC TCGCCCGACC ACCAGGGCAA 5701 GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC 5761 CGCCTTCCTG GAGACCTCCG CGCCCCGCAA CCTCCCCTTC TACGAGCGGC TCGGCTTCAC 5821 CGTCACCGCC GACGTCGAGG TGCCCGAAGG ACCGCGCACC TGGTGCATGA CCCGCAAGCC 5881 CGGTGCCTGA GTCGACAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT 5941 TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA 6001 TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC 6061 TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC 6121 TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT 6181 CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG 6241 GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AATCATCGTC 6301 CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA 6361 CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG 6421 GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC 6481 CCCGCCTGGT ACCTTTAAGA CCAATGACTT ACAAGGCAGC TGTAGATCTT AGCCACTTTT 6541 TAAAAGAAAA GGGGGGACTG GAAGGGCTAA TTCACTCCCA ACGAAAATAA GATCTGCTTT 6601 TTGCTTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC 6661 TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG 6721 CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA 6781 AAATCTCTAG CAGTAGTAGT TCATGTCATC TTATTATTCA GTATTTATAA CTTGCAAAGA 6841 AATGAATATC AGAGAGTGAG AGGAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA 6901 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT 6961 TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGCTCTA GCTATCCCGC CCCTAACTCC 7021 GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC 7081 CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT 7141 AGACTTTTGC AGAGACGGCC CAAATTCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA 7201 TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG 7261 GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA 7321 GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG 7381 TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG 7441 GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 7501 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 7561 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 7621 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 7681 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 7741 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 7801 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 7861 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 7921 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 7981 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC 8041 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 8101 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 8161 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 8221 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 8281 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA 8341 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT 8401 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG 8461 TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA 8521 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC 8581 TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT 8641 TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG 8701 CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT 8761 TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT 8821 GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT 8881 GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC 8941 TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT 9001 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG 9061 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT 9121 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG 9181 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA 9241 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC 9301 GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT 9361 AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG 9421 TGAAAACCTC TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC 9481 CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT 9541 TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC 9601 GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCCAT TCGCCATTCA GGCTGCGCAA 9661 CTGTTGGGAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCTGG CGAAAGGGGG 9721 ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA 9781 AACGACGGCC AGTGCCAAGC TG

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.