pLV3-CMV-KAT8-3×FLAG-CopGFP-Pu

pLV3-CMV-KAT8-3×FLAG-CopGFP-Puro plasmid

PVT94731

pLV3-CMV-KAT8-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-KAT8(human)-3×FLAG-CopGFP-Puro
Alias: NM_032188.3;hMOF; LIGOWS; MOF; MYST1; ZC2HC8
Gene lenght: 1374bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-KAT8-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-KAT8-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-KAT8-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg cggcacaggg agctgctgcg 2341 gcggttgcgg cggggacttc aggggtcgcg ggggagggcg agcccgggcc cggggagaat 2401 gcggccgctg aggggaccgc cccatccccg ggccgcgtct ctccgccgac cccggcgcgc 2461 ggcgagccgg aagtcacggt ggagatcgga gaaacgtacc tgtgccggcg accggatagc 2521 acctggcatt ctgctgaagt gatccagtct cgagtgaacg accaggaggg ccgagaggaa 2581 ttctatgtac actacgtggg ctttaaccgg cggctggacg agtgggtaga caagaaccgg 2641 ctggcgctga ccaagacagt gaaggatgct gtacagaaga actcagagaa gtacctgagc 2701 gagctcgcag agcagcctga gcgcaagatc actcgcaacc aaaagcgcaa gcatgatgag 2761 atcaaccatg tgcagaagac ttatgcagag atggacccca ccacagcagc cttggagaag 2821 gagcatgagg cgatcaccaa ggtgaagtat gtggacaaga tccacatcgg gaactacgaa 2881 attgatgcct ggtatttctc accattcccc gaagactatg ggaaacagcc caagctctgg 2941 ctctgcgagt actgcctcaa gtacatgaaa tatgagaaga gctaccgctt ccacttgggt 3001 cagtgccagt ggcggcagcc ccccgggaaa gagatctacc gcaagagcaa catctccgtg 3061 tacgaagttg atggcaaaga ccataagatt tactgtcaga acctgtgtct gctggccaag 3121 cttttcctgg accataagac actgtacttt gacgtggagc cgttcgtctt ttacatcctg 3181 actgaggtgg accggcaggg ggcccacatt gttggctact tctccaagga gaaggagtcc 3241 ccggatggaa acaatgtggc ctgcatcctg accttgcccc cctaccaacg ccgcggctac 3301 gggaagttcc tcatcgcttt cagttatgag ctctccaagc tggagagcac agtcggctcc 3361 ccggagaagc cactgtctga cctgggcaag ctcagctacc gcagctactg gtcctgggtg 3421 ctgctagaga tcctgcggga cttccggggc acactgtcca tcaaggacct cagccagatg 3481 accagtatca cccaaaatga catcatcagt accctgcaat ccctcaatat ggtcaagtac 3541 tggaagggcc agcacgtgat ctgtgtcaca cccaagctgg tggaggagca cctcaaaagt 3601 gcccagtata agaaaccacc catcacagtg gactccgtct gcctcaagtg ggcacccccc 3661 aagcacaagc aagtcaagct ctccaagaag ggatccGACT ACAAAGACCA TGACGGTGAT 3721 TATAAAGATC ATGACATCGA CTACAAGGAT GACGATGACA AGtagcggcc gcGaaggatc 3781 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag 3841 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg 3901 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata 3961 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg 4021 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat 4081 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc 4141 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg 4201 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac 4261 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg 4321 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc 4381 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc 4441 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc 4501 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc 4561 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag 4621 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc 4681 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc 4741 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg 4801 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg 4861 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc 4921 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg 4981 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc 5041 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga 5101 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc 5161 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg 5221 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc 5281 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 5341 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 5401 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 5461 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 5521 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 5581 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 5641 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 5701 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac 5761 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga 5821 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc 5881 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa 5941 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc 6001 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc 6061 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc 6121 gtggtgttgt cggggaaATC ATCGTCCTTT CCTtggctgc tcgcctgtgt tgccacctgg 6181 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct 6241 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tTcgccttcg ccctcagacg 6301 agtcggatct ccctttggGc cgcctccccg cctggtacct ttaagaccaa tgacttacaa 6361 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca 6421 ctcccaacga aGataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat 6481 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 6541 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 6601 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat 6661 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat 6721 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 6781 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 6841 gctctagcta TCCCGCCCCT AACTCCGCCC Atcccgcccc taactccgcc cagttccgcc 6901 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 6961 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga 7021 gaccaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat 7081 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 7141 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 7201 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 7261 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 7321 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 7381 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 7441 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 7501 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 7561 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 7621 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 7681 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 7741 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 7801 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 7861 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 7921 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 7981 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 8041 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 8101 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 8161 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 8221 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 8281 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 8341 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 8401 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 8461 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 8521 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 8581 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 8641 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 8701 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 8761 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 8821 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 8881 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 8941 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 9001 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 9061 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 9121 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 9181 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 9241 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 9301 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 9361 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 9421 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 9481 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 9541 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 9601 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 9661 gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.