pCMV-3×FLAG-TTC7A-Neo plasmid

pCMV-3×FLAG-TTC7A-Neo plasmid

PVT87669

pCMV-3×FLAG-TTC7A-Neo plasmid Information

Plasmid name: pCMV-3×FLAG-TTC7A(human)-Neo
Alias: NM_020458.4;GIDID; MINAT; TTC7
Gene lenght: 2577bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pCMV-T7-3×FLAG-MCS-Neo
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×FLAG-TTC7A-Neo plasmid Description

pCMV-3×FLAG-TTC7A-Neo This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a CMV enhancer and a CMV promoter that can jointly initiate the efficient expression of the human TTC7A gene, promoting the N-terminal fusion expression of the TTC7A gene with a 3×FLAG tag, facilitating the detection, localization, and purification of the expressed fusion protein using FLAG antibodies. The plasmid carries a Neo/G418 resistance gene for selecting host cells that express the target gene.

pCMV-3×FLAG-TTC7A-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac 961 atcgactaca aggatgacga tgacaagctt atggctgcga agggcgcgca cggctcctac 1021 ctgaaggtgg agagcgagct ggagcgctgc cgcgccgagg gccactggga ccgcatgccg 1081 gagctggtcc ggcagctgca gacgctgagc atgcccggcg gcggaggtaa caggcgaggc 1141 agcccgagcg cagcgttcac ctttccggac accgatgact ttgggaaatt gctgctggct 1201 gaggccctcc tggagcagtg tttgaaggag aaccatgcca aaataaaaga ctccatgcct 1261 ttgctggaga agaatgagcc gaagatgagc gaagccaaaa attatctaag cagtatcctt 1321 aaccatggga ggctctcgcc acagtacatg tgtgaggcca tgctgatcct gggcaaactg 1381 cattacgtgg agggctcata ccgagatgcc atcagcatgt acgcacgggc cgggattgat 1441 gacatgtcca tggagaacaa gcccctgtat cagatgcggc tgctgtcgga ggcttttgtc 1501 atcaaaggcc tctctctgga acgcctaccc aactccatcg cctcccgctt ccgcctgaca 1561 gagagggagg aggaagtgat cacctgtttt gagagggcct cctggatcgc tcaggtgttc 1621 ctgcaggaat tggagaagac cacaaataac agcacgtcga ggcatctgaa aggctgtcac 1681 ccgcttgact atgagctcac ctacttcctg gaagctgccc tccagagcgc ctatgtgaaa 1741 aacctgaaga aggggaacat cgtgaagggc atgagagagc tccgggaggt gctgcggact 1801 gtggagacca aagcaactca gaacttcaaa gtgatggcgg ccaagcacct ggcgggggtc 1861 ctgctgcact ccctgagtga ggagtgctac tggagccccc tgtcccaccc tctgcctgag 1921 ttcatgggca aggaggagag ttctttcgcc actcaggccc tgcggaaacc tcacctctat 1981 gaaggagaca acctctactg ccccaaggac aacatcgagg aagccctcct gctcctcctc 2041 atcagcgaat ccatggcaac tcgagatgtg gtgctgagcc gggtgccgga gcaggaggag 2101 gaccggacag tgagcttgca gaatgccgca gccatctatg acctcctgag catcacgttg 2161 ggcagaaggg gacagtacgt catgctctcg gagtgcctgg agcgagccat gaagtttgcg 2221 tttggagaat ttcacctttg gtaccaggtg gccctctcca tggtggcttg tgggaagtca 2281 gcctacgctg tgtccctgct gcgggagtgt gtgaagttgc ggccctcgga ccccaccgtg 2341 cccctgatgg ccgcgaaggt ctgcatcggg tcccttcgct ggctagagga agcagagcac 2401 tttgccatga tggtgatcag cctcggagag gaagccgggg agttcctccc caagggctac 2461 ctggctctgg gtctcaccta tagcctgcag gccaccgacg ccaccctgaa gtccaagcaa 2521 gatgaattgc accggaaggc actgcagacg ctggagaggg ctcagcagct ggcgcccagt 2581 gacccccagg tcatcctcta tgtctcgctg cagctggccc tcgtccgaca gatctccagt 2641 gccatggagc agctgcagga ggccctgaag gtacgcaagg atgatgccca cgccctccac 2701 ctgctggcac tgctcttctc tgcccagaag caccaccagc atgccctgga tgttgtcaac 2761 atggccatca ccgagcaccc tgagaacttc aacctgatgt tcaccaaggt gaagctggag 2821 caggtgctga aaggcccaga ggaagccctc gtgacctgca gacaagtgct gaggctgtgg 2881 cagaccctgt acagcttctc ccagctggga ggcctagaaa aggatggcag cttcggtgag 2941 ggcctcacca tgaagaagca gagtggcatg cacctgactt tgcctgatgc ccatgatgca 3001 gactctggct cccggcgggc ttcgtccatc gccgcctccc ggctggagga ggccatgtca 3061 gagctgacta tgccctcttc ggtcctgaag cagggcccca tgcagctgtg gaccacgctg 3121 gaacagatct ggctgcaggc tgctgagctg ttcatggagc agcagcacct caaggaagca 3181 ggtttctgca tccaggaggc ggcgggcctc ttccccactt ctcactcagt actctatatg 3241 cggggccggc tggctgaggt gaagggcaac ctggaggagg ccaagcagct gtacaaggag 3301 gcgctcacgg tgaacccaga tggcgtgcgc atcatgcata gcctgggtct gatgctgagt 3361 cggctgggcc acaagagctt ggcccagaag gtgcttcgtg atgccgtgga gaggcagagt 3421 acgtgccacg aggcgtggca gggcctgggc gaggtgctgc aggcccaggg ccagaacgag 3481 gctgccgttg actgcttcct caccgccctt gagctggagg ccagcagccc tgtactgccc 3541 ttctccatca tccccagaga gctctgagaa ttctgcagat atccagcaca gtggcggccg 3601 ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc 3661 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc 3721 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct 3781 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg 3841 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct 3901 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 3961 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 4021 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta 4081 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 4141 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg 4201 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat 4261 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt 4321 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt 4381 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 4441 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 4501 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 4561 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 4621 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt 4681 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag 4741 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg 4801 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt 4861 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc 4921 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt 4981 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag 5041 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg 5101 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag 5161 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg 5221 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc 5281 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca 5341 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc 5401 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg 5461 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct 5521 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc 5581 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg 5641 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct 5701 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa 5761 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 5821 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc 5881 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 5941 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 6001 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 6061 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 6121 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 6181 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 6241 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 6301 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 6361 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 6421 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 6481 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 6541 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 6601 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 6661 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 6721 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 6781 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg 6841 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 6901 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 6961 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 7021 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 7081 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 7141 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 7201 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 7261 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 7321 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 7381 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 7441 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 7501 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 7561 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 7621 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 7681 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 7741 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 7801 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 7861 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 7921 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 7981 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 8041 cgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.