pCMV-PVR-3×FLAG-EGFP-Neo plasm

pCMV-PVR-3×FLAG-EGFP-Neo plasmid

PVT96833

pCMV-PVR-3×FLAG-EGFP-Neo plasmid Information

Plasmid name: pCMV-PVR(human)-3×FLAG-EGFP-Neo
Alias: NM_006505.5;CD155; HVED; Necl-5; NECL5; PVS; TAGE4
Gene lenght: 1251bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-PVR-3×FLAG-EGFP-Neo plasmid Description

pCMV-PVR-3×FLAG-EGFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-PVR-3×FLAG-EGFP-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaagcc actgggatcc GCCACCatgg cccgagccat ggccgccgcg tggccgctgc 961 tgctggtggc gctactggtg ctgtcctggc cacccccagg aaccggggac gtcgtcgtgc 1021 aggcgcccac ccaggtgccc ggcttcttgg gcgactccgt gacgctgccc tgctacctac 1081 aggtgcccaa catggaggtg acgcatgtgt cacagctgac ttgggcgcgg catggtgaat 1141 ctggcagcat ggccgtcttc caccaaacgc agggccccag ctattcggag tccaaacggc 1201 tggaattcgt ggcagccaga ctgggcgcgg agctgcggaa tgcctcgctg aggatgttcg 1261 ggttgcgcgt agaggatgaa ggcaactaca cctgcctgtt cgtcacgttc ccgcagggca 1321 gcaggagcgt ggatatctgg ctccgagtgc ttgccaagcc ccagaacaca gctgaggttc 1381 agaaggtcca gctcactgga gagccagtgc ccatggcccg ctgcgtctcc acagggggtc 1441 gcccgccagc ccaaatcacc tggcactcag acctgggcgg gatgcccaat acgagccagg 1501 tgccagggtt cctgtctggc acagtcactg tcaccagcct ctggatattg gtgccctcaa 1561 gccaggtgga cggcaagaat gtgacctgca aggtggagca cgagagcttt gagaagcctc 1621 agctgctgac tgtgaacctc accgtgtact accccccaga ggtatccatc tctggctatg 1681 ataacaactg gtaccttggc cagaatgagg ccaccctgac ctgcgatgct cgcagcaacc 1741 cagagcccac aggctataat tggagcacga ccatgggtcc cctgccaccc tttgctgtgg 1801 cccagggcgc ccagctcctg atccgtcctg tggacaaacc aatcaacaca actttaatct 1861 gcaacgtcac caatgcccta ggagctcgcc aggcagaact gaccgtccag gtcaaagagg 1921 gacctcccag tgagcactca ggcatgtccc gtaacgccat catcttcctg gttctgggaa 1981 tcctggtttt tctgatcctg ctggggatcg ggatttattt ctattggtcc aaatgttccc 2041 gtgaggtcct ttggcactgt catctgtgtc cctcgagtac agagcatgcc agcgcctcag 2101 ctaatgggca tgtctcctat tcagctgtga gcagagagaa cagctcttcc caggatccac 2161 agacagaggg cacaaggGAC TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG 2221 ACTACAAGGA TGACGATGAC AAGGCctcga gtggcggtgg aggaagcggc ggaggtggat 2281 ctatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg 2341 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct 2401 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca 2461 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga 2521 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct 2581 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc 2641 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc 2701 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga 2761 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg 2821 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc 2881 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg 2941 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt 3001 agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc 3061 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 3121 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 3181 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg 3241 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg 3301 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca 3361 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct 3421 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt 3481 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac 3541 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct 3601 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt 3661 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac 3721 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc 3781 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg 3841 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 3901 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc 3961 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 4021 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa 4081 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc 4141 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag 4201 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg 4261 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa 4321 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc 4381 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc 4441 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga 4501 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa 4561 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct 4621 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat 4681 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt 4741 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta 4801 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga 4861 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg 4921 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg 4981 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc 5041 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag 5101 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc 5161 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 5221 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa 5281 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 5341 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 5401 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 5461 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 5521 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 5581 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 5641 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 5701 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 5761 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 5821 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 5881 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 5941 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 6001 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 6061 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 6121 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 6181 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt tgtttgcaag 6241 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 6301 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 6361 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 6421 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 6481 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 6541 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 6601 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 6661 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 6721 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 6781 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 6841 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 6901 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 6961 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 7021 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 7081 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 7141 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 7201 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 7261 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 7321 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 7381 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.