pLV2-CMV-Stag2-3×FLAG-Puro pla

pLV2-CMV-Stag2-3×FLAG-Puro plasmid

PVT94803

pLV2-CMV-Stag2-3×FLAG-Puro plasmid Information

Plasmid name: pLV2-CMV-Stag2(mouse)-3×FLAG-Puro
Alias: Inducing agents should be added during extraction
Gene lenght: 3693bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): EPI300
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: PGK-R
Copy number: Low
Induction:

pLV2-CMV-Stag2-3×FLAG-Puro plasmid Description

pLV2-CMV-Stag2-3×FLAG-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-CMV-Stag2-3×FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatgatagc agctccagaa ataccaactg attttaatct 2881 actgcaggag tcagagacac acttttcttc tgacacagat tttgaagaca ttgaaggaaa 2941 aaatcagaag caaggcaaag gcaaaacttg taaaaaaggc aaaaagggtc cagcagaaaa 3001 gggtaaaagt ggaaatggag gaggaaaacc tccttctggc tcaaaccgaa tgaacggtca 3061 tcatcaacag aatggagttg aaaacatgat gttgttcgaa gttgttaaaa tgggcaagag 3121 tgctatgcag tcggtggtag atgactggat cgagtcctac aagcatgacc gggacatagc 3181 gcttcttgac cttatcaatt tctttattca gtgctcaggc tgcaaaggag tcgtcacagc 3241 agagatgttc agacatatgc agaactctga gataattcga aaaatgacag aagaatttga 3301 cgaggatagt ggggattacc cacttaccat ggctggtcct cagtggaaga agttcaagtc 3361 cagcttctgt gagttcattg gtgtgttagt acggcagtgt caatacagta tcatatatga 3421 tgagtacatg atggatactg tcatttcact tcttactgga ttgtctgact cccaagtcag 3481 agcatttcga catacaagca ccctggcagc tatgaagttg atgactgctt tggtaaatgt 3541 ggcactaaat cttagcatta atatggataa cacacaaaga cagtacgagg cagaacggaa 3601 taaaatgatt ggaaaacgag ctaacgaaag gctagaactc ctgttacaaa agcgtaaaga 3661 gcttcaggaa aatcaagatg agatagaaaa tatgatgaat gcaatattta aaggagtgtt 3721 tgtacataga tacagggatg caatagctga aatcagagct atatgcattg aagagattgg 3781 catttggatg aagatgtata gtgatgcctt ccttaatgac agctatttaa aatatgttgg 3841 ttggactatg catgataagc aaggtgaagt cagactcaaa tgtcttactg ctttacaagg 3901 gctttactat aacaaagaac ttaattccaa actggaactc tttaccagtc gattcaagga 3961 tagaattgtg tctatgaccc ttgacaaaga atatgatgtt gcagtacaag caataaagtt 4021 actcactctt gttttacaga gtagtgaaga agttctaact gcagaagatt gtgaaaatgt 4081 ctatcatctg gtttattcag ctcaccggcc agtagcagtt gcagctggag aatttctcta 4141 caaaaagctc ttcagccgca gagacccaga agaggatggg ctgatgaaaa gaaggggaag 4201 gcaaggcccg aatgccaacc ttgtgaagac tttggtgttt ttctttttgg aaagtgagtt 4261 acacgagcat gcagcttacc ttgtggacag catgtgggat tgtgctactg agctcctgaa 4321 agactgggaa tgtatgaata gcttgttatt agaagagcct cttagtggag aggaagctct 4381 aacagacaga caagagagtg ctttgattga aataatgctt tgtactatta gacaagcagc 4441 tgaatgtcat cctcccgtgg gaagagggac aggaaaaagg gtgctgacag caaaggagaa 4501 gaagacacag ttggatgaca gaacaagaat cactgagctg tttgctgtgg cccttccgca 4561 gttattagct aaatactctg tagatgcaga aaaggtgacc aatttgttgc agttacctca 4621 gtactttgat ttggagatat acaccactgg acgattagaa aagcatttgg atgccttatt 4681 gcgccagatc cgaaatattg tagagaagca cacagacaca gatgttttgg aagcatgttc 4741 taaaacttac catgcattat gtaacgaaga gttcaccatc ttcaacagag tagatatttc 4801 aagaagtcag ctgattgatg aactggcaga taaatttaac cggcttcttg aagattttct 4861 gcaagaggga gaagaacctg atgaagatga cgcatatcag gtattgtcaa cattgaagag 4921 gatcactgct tttcataatg ctcatgatct ttcaaagtgg gatttgttcg cttgtaatta 4981 caaactcttg aaaaccggaa tcgaaaatgg agatatgcct gagcagattg ttattcacgc 5041 actgcagtgt gctcactatg taatcctttg gcagcttgct aagatcactg aaagcacgtc 5101 tacaaaggag gacttactgc gcttaaagaa acagatgagg gtattttgcc agatatgtca 5161 gcattacttg accaacgtga atactactgt taaagaacag gccttcacta ttctgtgtga 5221 cattttgatg atcttcagtc accagattat gtctggaggg cgtgacatgt tagagccatt 5281 agtttatact cctgattctt cattgcagtc tgagttgctc agctttattt tggatcatgt 5341 cttcattgaa caggatgatg atagtaatag tgcagatggt cagcaagagg atgaagccag 5401 caaaattgaa gctttgcaca agaggagaaa tttacttgca gcattttgta agctaattgt 5461 atataccgtg gtggagatga acacagctgc agatatcttc aagcagtata tgaagtatta 5521 taatgattat ggcgatatca tcaaagaaac aatgagtaaa acaaggcaga ttgacaaaat 5581 tcagtgtgct aagaccctta ttctcagttt gcagcagctt tttaatgaga tgatacaaga 5641 gaatggctat aattttgata gatcatcctc aacatttagt ggcataaaag aacttgctcg 5701 gcgttttgct ttaacttttg gacttgatca attgaaaacc agagaagcca ttgccatgct 5761 ccacaaagat ggtatagaat ttgcttttaa ggagcctaat ccacaagggg agagccatcc 5821 acctttaaac ttggcatttc ttgatattct cagtgaattt tcttctaaac tgctccgaca 5881 ggataaaaga acagtgtatg tttatttgga aaagttcatg acctttcaga tgtcactccg 5941 aagagaagat gtatggcttc ctctgatgtc ttaccgaaat tctttgctag ctggtggtga 6001 tgatgatact atgtcagtca ttagtggaat gagcagtcgg ggatcaactg tacggagtaa 6061 aaaatcaaaa ccatctacag gaaagcggaa agtggttgaa ggcatgcaac tagcactccc 6121 tgaagaaagc agtagcagtg acagtatgtg gttgagcaga gagcaaacac tgcacacccc 6181 tgtcatgatg cagacaccac agctcacctc cactattatg agagaaccga aaaggttacg 6241 gcctgaagat agcttcatga gtgtctatcc gatgcaggca gaacaccatc aaacgcctct 6301 ggattataat cggcgtggta caagcctaat ggaagatgat gaagagccaa ttgttgaaga 6361 tgttatgatg tcctcagaag gaaggattga ggatcttaat gagggaatgg actttgacac 6421 catggatata gacttgccac catcaaagaa tagaagagag agaacagaac tgaagcccga 6481 tttctttgat ccagcttcaa ttatggatga atcagttctt ggggtgtcaa tgtttggatc 6541 cGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC ATCGACTACA AGGATGACGA 6601 TGACAAGtag tctagataat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg 6661 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg 6721 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc 6781 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg 6841 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc 6901 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc 6961 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag 7021 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg 7081 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag 7141 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg 7201 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat 7261 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg 7321 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg 7381 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg 7441 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc 7501 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt 7561 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc 7621 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc 7681 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt 7741 gcctgaccgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg 7801 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 7861 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 7921 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 7981 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac 8041 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 8101 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac 8161 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg 8221 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 8281 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc 8341 ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt 8401 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag 8461 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta 8521 gatcttagcc actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga 8581 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag 8641 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta 8701 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta 8761 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt 8821 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac 8881 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc 8941 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc 9001 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 9061 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 9121 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 9181 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 9241 aatatcagag agtgagaggc cttgacattg ctagcgtttt accgtcgacc tctagctaga 9301 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 9361 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 9421 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 9481 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 9541 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 9601 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 9661 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 9721 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 9781 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 9841 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 9901 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 9961 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 10021 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 10081 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 10141 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 10201 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 10261 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 10321 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 10381 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 10441 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 10501 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 10561 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 10621 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 10681 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 10741 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 10801 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 10861 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 10921 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 10981 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 11041 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 11101 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 11161 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 11221 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 11281 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 11341 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 11401 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 11461 tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac 11521 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 11581 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa 11641 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg 11701 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt 11761 atttgttaaa tatgtactac aaacttagta gtttttaaag aaattgtatt tgttaaatat 11821 gtactacaaa cttagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.