pCAGGS-FLAG-IKBKG plasmid

pCAGGS-FLAG-IKBKG plasmid

PVT94763

pCAGGS-FLAG-IKBKG plasmid Information

Plasmid name: pCAGGS-FLAG-IKBKG(human)
Alias: NM_001321396.3;AMCBX1; EDAID1; FIP-3; FIP3; Fip3p;IKK-gamma; IKKAP1; IKKG; IMD33; IP; IP1; IP2; IPD2; NEMO;SAIDX; ZC2HC9
Gene lenght: 1260bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CAG
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pcaggs-F
Reverse primer:
Copy number: High
Induction:

pCAGGS-FLAG-IKBKG plasmid Description

pCAGGS-FLAG-IKBKG is a plasmid suitable for protein expression in mammalian cells.

pCAGGS-FLAG-IKBKG plasmid Sequence

1 GTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA 61 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC 121 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG 181 GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC 241 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG 301 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG 361 TATTAGTCAT CGCTATTACC ATGGTCGAGG TGAGCCCCAC GTTCTGCTTC ACTCTCCCCA 421 TCTCCCCCCC CTCCCCACCC CCAATTTTGT ATTTATTTAT TTTTTAATTA TTTTGTGCAG 481 CGATGGGGGC GGGGGGGGGG GGGGGGCCCC CCCCAGGCGG GGCGGGGCGG GGCGAGGGGC 541 GGGGCGGGGC GAGGCGGAAA GGTGCGGCGG CAGCCAATCA GAGCGGCGCG CTCCGAAAGT 601 TTCCTTTTAT GGCGAGGCGG CGGCGGCGGC GGCCCTATAA AAAGCGAAGC GCGCGGCGGG 661 CGGGAGTCGT TGCGCGCTGC CTTCCCCCCG TGCCCCGCTC CGCCGCCGCC TCGCGCCGCC 721 CGCCCCGGCT CTGACTGACC GCGTTACTCC CACAGGTGAG CGGGCGGGAC GGCCCTTCTC 781 CTCCGGGCTG TAATTAGCGC TTGGTTTAAT GACGGCTTGT TTCTTTTCTG TGGCTGCGTG 841 AAAGCCTTGA GGGGCTCCGG GAGGGCCCTT TGTGCGGGGG GAGCGGCTCG GGGGGTGCGT 901 GCGTGTGTGT GTGCGTGGGG AGCGCCGCGT GCGGCTCCGC GCTGCCCGGC GGCTGTGAGC 961 GCTGCGGGCG CGGCGCGGGG CTTTGTGCGC TCCGCAGTGT GCGCGAGGGG AGCGCGGCCG 1021 GGGGCGGTGC CCCGCGGTGC GGGGGGGGCT GCGAGGGGAA CAAAGGCTGC GTGCGGGGTG 1081 TGTGCGTGGG GGGGTGAGCA GGGGGTGTGG GCGCGTCGGT CGGGCTGCAA CCCCCCCTGC 1141 ACCCCCCTCC CCGAGTTGCT GAGCACGGCC CGGCTTCGGG TGCGGGGCTC CGTACGGGGC 1201 GTGGCGCGGG GCTCGCCGTG CCGGGCGGGG GGTGGCGGCA GGTGGGGGTG CCGGGCGGGG 1261 CGGGGCCGCC TCGGGCCGGG GAGGGCTCGG GGGAAGGGGC GCGGCGGCCC CCGGAGCGCC 1321 GGCGGCTGTC GAGGCGCGGC GAGCCGCAGC CATTGCCTTT TATGGTAATC GTGCGAGAGG 1381 GCGCAGGGAC TTCCTTTGTC CCAAATCTGT GCGGAGCCGA AATCTGGGAG GCGCCGCCGC 1441 ACCCCCTCTA GCGGGCGCGG GGCGAAGCGG TGCGGCGCCG GCAGGAAGGA AATGGGCGGG 1501 GAGGGCCTTC GTGCGTCGCC GCGCCGCCGT CCCCTTCTCC CTCTCCAGCC TCGGGGCTGT 1561 CCGCGGGGGG ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT 1621 GTGACCGGCG GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG 1681 CTCCTGGGCA ACGTGCTGGT TGTTGTGCTG TCTCATCATT TTGGCAAAGC CACCATGGAT 1741 TACAAGGATG ACGACGATAA GGAATTCatg aataggcacc tctggaagag ccaactgtgt 1801 gagatggtgc agcccagtgg tggcccggca gcagatcagg acgtactggg cgaagagtct 1861 cctctgggga agccagccat gctgcacctg ccttcagaac agggcgctcc tgagaccctc 1921 cagcgctgcc tggaggagaa tcaagagctc cgagatgcca tccggcagag caaccagatt 1981 ctgcgggagc gctgcgagga gcttctgcat ttccaagcca gccagaggga ggagaaggag 2041 ttcctcatgt gcaagttcca ggaggccagg aaactggtgg agagactcgg cctggagaag 2101 ctcgatctga agaggcagaa ggagcaggct ctgcgggagg tggagcacct gaagagatgc 2161 cagcagcaga tggctgagga caaggcctct gtgaaagccc aggtgacgtc cttgctcggg 2221 gagctgcagg agagccagag tcgcttggag gctgccacta aggaatgcca ggctctggag 2281 ggtcgggccc gggcggccag cgagcaggcg cggcagctgg agagtgagcg cgaggcgctg 2341 cagcagcagc acagcgtgca ggtggaccag ctgcgcatgc agggccagag cgtggaggcc 2401 gcgctccgca tggagcgcca ggccgcctcg gaggagaaga ggaagctggc ccagttgcag 2461 gtggcctatc accagctctt ccaagaatac gacaaccaca tcaagagcag cgtggtgggc 2521 agtgagcgga agcgaggaat gcagctggaa gatctcaaac agcagctcca gcaggccgag 2581 gaggccctgg tggccaaaca ggaggtgatc gataagctga aggaggaggc cgagcagcac 2641 aagattgtga tggagaccgt tccggtgctg aaggcccagg cggatatcta caaggcggac 2701 ttccaggctg agaggcaggc ccgggagaag ctggccgaga agaaggagct cctgcaggag 2761 cagctggagc agctgcagag ggagtacagc aaactgaagg ccagctgtca ggagtcggcc 2821 aggatcgagg acatgaggaa gcggcatgtc gaggtctccc aggccccctt gccccccgcc 2881 cctgcctacc tctcctctcc cctggccctg cccagccaga ggaggagccc ccccgaggag 2941 ccacctgact tctgctgtcc caagtgccag tatcaggccc ctgatatgga caccctgcag 3001 atacatgtca tggagtgcat tgagtagCTC GAGCTAGCAG ATCTTAATTA ATTAAGGATC 3061 TTTTTCCCTC TGCCAAAAAT TATGGGGACA TCATGAAGCC CCTTGAGCAT CTGACTTCTG 3121 GCTAATAAAG GAAATTTATT TTCATTGCAA TAGTGTGTTG GAATTTTTTG TGTCTCTCAC 3181 TCGGAAGGAC ATATGGGAGG GCAAATCATT TAAAACATCA GAATGAGTAT TTGGTTTAGA 3241 GTTTGGCAAC ATATGCCCAT ATGCTGGCTG CCATGAACAA AGGTTGGCTA TAAAGAGGTC 3301 ATCAGTATAT GAAACAGCCC CCTGCTGTCC ATTCCTTATT CCATAGAAAA GCCTTGACTT 3361 GAGGTTAGAT TTTTTTTATA TTTTGTTTTG TGTTATTTTT TTCTTTAACA TCCCTAAAAT 3421 TTTCCTTACA TGTTTTACTA GCCAGATTTT TCCTCCTCTC CTGACTACTC CCAGTCATAG 3481 CTGTCCCTCT TCTCTTATGG AGATCCCTCG ACCTGCAGCC CAAGCTTGGC GTAATCATGG 3541 TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC 3601 GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG 3661 TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCGGAT CCGCATCTCA 3721 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA 3781 GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG 3841 CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT 3901 TTTGCAAAAA GCTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT 3961 CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT 4021 CATCAATGTA TCTTATCATG TCTGGATCCG CTGCATTAAT GAATCGGCCA ACGCGCGGGG 4081 AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG 4141 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA 4201 GAATCAGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 4261 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 4321 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 4381 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 4441 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 4501 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 4561 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 4621 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 4681 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 4741 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 4801 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 4861 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 4921 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 4981 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 5041 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 5101 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 5161 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 5221 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 5281 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 5341 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 5401 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 5461 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 5521 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 5581 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 5641 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 5701 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 5761 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 5821 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 5881 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 5941 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 6001 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.