pLV3-CMV-KHDC4-3×Myc-EF1a-CopG

pLV3-CMV-KHDC4-3×Myc-EF1a-CopGFP-Puro plasmid

PVT90513

pLV3-CMV-KHDC4-3×Myc-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-KHDC4(human)-3×Myc-EF1a-CopGFP-Puro
Alias: NM_014949.4;BLOM7; KIAA0907; SNORA80EHG
Gene lenght: 1842bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-KHDC4-3×Myc-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-KHDC4-3×Myc-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-KHDC4-3×Myc-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc GCCAccatgt ccgcggggag cgcgacacat cctggagctg gcgggcgccg 2341 cagcaaatgg gaccaaccag ctccagcccc acttctcttc ctcccgccag cggccccagg 2401 tggggaggtc accagcagtg ggggaagtcc tgggggcacc acagctgctc cttcaggagc 2461 cttggatgct gctgctgctg tggctgccaa gattaatgcc atgctcatgg caaaagggaa 2521 gctgaaacca actcagaatg cttctgagaa gcttcaggct cctggcaaag gcctaactag 2581 caataaaagc aaggatgacc tggtggtagc tgaagtagaa attaatgatg tgcctctcac 2641 atgtaggaac ttgctgactc gaggacagac tcaagacgag atcagccgac ttagtggggc 2701 tgcagtatca actcgaggga ggttcatgac aactgaggaa aaagccaaag tgggaccagg 2761 ggatcgtcca ttatatcttc atgttcaggg ccagacacgg gaattagtgg acagagctgt 2821 aaaccggatc aaagaaatta tcaccaatgg agtggtaaaa gctgccacag gaacaagtcc 2881 aacttttaat ggtgcaacag taactgtcta tcaccagcca gcacccatcg ctcagttgtc 2941 tccagctgtt agccagaagc ctcccttcca gtcagggatg cattatgttc aagataaatt 3001 atttgtgggt ctagaacatg ctgtacccac ttttaatgtc aaggagaagg tggaaggtcc 3061 aggctgctcc tatttgcagc acattcagat tgaaacaggt gccaaagtct tcctgcgggg 3121 caaaggttca ggctgcattg agccagcatc tggccgagaa gcttttgaac ctatgtatat 3181 ttacatcagt caccccaaac cagaaggcct ggctgctgcc aagaagcttt gtgagaatct 3241 tttgcaaaca gttcatgctg aatactctag atttgtgaat cagattaata ctgctgtacc 3301 tttaccaggc tatacacaac cctctgctat aagtagtgtc cctcctcaac caccatatta 3361 tccatccaat ggctatcagt ctggttaccc tgttgttccc cctcctcagc agccagttca 3421 acctccctac ggagtaccaa gcatagtgcc accagctgtt tcattagcac ctggagtctt 3481 gccggcatta cctactggag tcccacctgt gccaacacaa tacccgataa cacaagtgca 3541 gcctccagct agcactggac agagtccgat gggtggtcct tttattcctg ctgctcctgt 3601 caaaactgcc ttgcctgctg gcccccagcc ccagccccag ccccagcccc cactcccaag 3661 tcagccccag gcacagaaga gacgattcac agaggagcta ccagatgaac gggaatctgg 3721 actgcttgga taccagcatg gacccattca tatgactaat ttaggtacag gcttctccag 3781 tcagaatgag attgaaggtg caggatcgaa gccagcaagt tcctcaggca aagagagaga 3841 gagggacagg cagttgatgc ctccaccagc ctttccagtg actggaataa aaacagagtc 3901 cgatgaaagg aatgggtctg ggaccttaac agggagccat gattatccag ccaagaagat 3961 gaaaactaca gagaagggat ttggcttggt ggcttatgct gcagattcat ctgatgaaga 4021 ggaggaacat ggaggtcata aaaatgcaag tagttttcca cagggctgga gtttgggata 4081 ccaatatcct tcatcacaac cacgagctaa acaacagatg ccattctgga tggctcccgg 4141 atccGAACAA AAACTAATAT CAGAGGAAGA TTTGGAACAG AAACTAATCA GTGAAGAAGA 4201 TTTAGAGCAA AAGTTAATTT CCGAGGAGGA CTTATAgcgg ccgcGaagga tctgcgatcg 4261 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg 4321 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga 4381 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt 4441 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg 4501 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg 4561 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta 4621 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc 4681 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt 4741 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc 4801 caccatggag agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg 4861 caccctgaac ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg 4921 ccgcatgacc aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct 4981 gagccacgtg atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa 5041 ccccttcctg cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga 5101 ggacggcggc gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg 5161 cgacttcaag gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat 5221 catccgcagc aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg 5281 cagcttcgcc cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag 5341 ccacatgcac ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt 5401 cgccttccgc cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca 5461 gcacgccttc aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc 5521 cgtggacggc accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct 5581 aacatgcggt gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg 5641 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga 5701 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct 5761 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga 5821 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc 5881 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat 5941 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg 6001 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga 6061 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc 6121 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg 6181 cacctggtgc atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg 6241 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 6301 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 6361 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 6421 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 6481 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 6541 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 6601 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg 6661 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 6721 cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat 6781 ctccctttgg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt 6841 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 6901 aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 6961 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 7021 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 7081 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 7141 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 7201 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 7261 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 7321 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 7381 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 7441 ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag 7501 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 7561 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 7621 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 7681 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 7741 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 7801 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 7861 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 7921 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 7981 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 8041 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 8101 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 8161 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 8221 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 8281 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 8341 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 8401 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 8461 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 8521 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 8581 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 8641 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 8701 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 8761 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 8821 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 8881 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 8941 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 9001 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 9061 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 9121 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 9181 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 9241 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 9301 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 9361 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 9421 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 9481 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 9541 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 9601 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc 9661 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg 9721 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct 9781 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc 9841 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat 9901 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg 9961 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 10021 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 10081 cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.