pLV3-CMV-KAT5-2-3×FLAG-CopGFP-

pLV3-CMV-KAT5-2-3×FLAG-CopGFP-Puro plasmid

PVT94747

pLV3-CMV-KAT5-2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-KAT5(human)-2-3×FLAG-CopGFP-Puro
Alias: NM_182710.3;cPLA2; ESA1; HTATIP; HTATIP1; NEDFASB;PLIP; TIP; TIP60; ZC2HC5
Gene lenght: 1638bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-KAT5-2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-KAT5-2-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-KAT5-2-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCATGG CGGAGGTGGT GAGTCCGGTG 2341 CCCGGGGCGG GGCGGAGGGA GCCAGGGGAG GTGGGTAGAG CCCGAGGCCC CCCAGTAGCC 2401 GACCCTGGCG TCGCGCTGTC TCCCCAGGGG GAGATAATCG AGGGCTGCCG CCTACCCGTG 2461 CTGCGGCGGA ACCAGGACAA CGAAGATGAG TGGCCCCTGG CCGAGATCCT GAGCGTGAAG 2521 GACATCAGTG GCCGGAAGCT TTTCTACGTC CATTACATTG ACTTCAACAA ACGTCTGGAT 2581 GAATGGGTGA CGCATGAGCG GCTGGACCTA AAGAAGATCC AGTTCCCCAA GAAAGAGGCC 2641 AAGACCCCCA CTAAGAACGG ACTTCCTGGG TCCCGTCCTG GCTCTCCAGA GAGAGAGGTG 2701 CCGGCCTCGG CGCAGGCCAG CGGGAAGACC TTGCCAATCC CGGTCCAGAT CACACTCCGC 2761 TTCAACCTGC CCAAGGAGCG GGAGGCCATT CCCGGTGGCG AGCCTGACCA GCCGCTCTCC 2821 TCCAGCTCCT GCCTGCAGCC CAACCACCGC TCAACGAAAC GGAAGGTGGA GGTGGTTTCA 2881 CCAGCAACTC CAGTGCCCAG CGAGACAGCC CCGGCCTCGG TTTTTCCCCA GAATGGAGCC 2941 GCCCGTAGGG CAGTGGCAGC CCAGCCAGGA CGGAAGCGAA AATCGAATTG TTTGGGCACT 3001 GATGAGGACT CCCAGGACAG CTCTGATGGA ATACCGTCAG CACCACGCAT GACTGGCAGC 3061 CTGGTGTCTG ATCGAAGCCA CGACGACATC GTCACCCGGA TGAAGAACAT TGAGTGCATT 3121 GAGCTGGGCC GGCACCGCCT CAAGCCGTGG TACTTCTCCC CGTACCCACA GGAACTCACC 3181 ACATTGCCTG TCCTCTACCT GTGCGAGTTC TGCCTCAAGT ACGGCCGTAG TCTCAAGTGT 3241 CTTCAGCGTC ATTTGACCAA GTGTGACCTA CGACATCCTC CAGGCAATGA GATTTACCGC 3301 AAGGGCACCA TCTCCTTCTT TGAGATTGAT GGACGTAAGA ACAAGAGTTA TTCCCAGAAC 3361 CTGTGTCTTT TGGCCAAGTG TTTCCTTGAC CATAAGACAC TGTACTATGA CACAGACCCT 3421 TTCCTCTTCT ACGTCATGAC AGAGTATGAC TGTAAGGGCT TCCACATCGT GGGCTACTTC 3481 TCCAAGGAGA AAGAATCAAC GGAAGACTAC AATGTGGCCT GCATCCTAAC CCTGCCTCCC 3541 TACCAGCGCC GGGGCTACGG CAAGCTGCTG ATCGAGTTCA GCTATGAACT CTCCAAAGTG 3601 GAAGGGAAAA CAGGGACCCC TGAGAAGCCC CTCTCAGACC TTGGCCTCCT ATCCTATCGA 3661 AGCTACTGGT CCCAGACCAT CCTGGAGATC CTGATGGGGC TGAAGTCGGA GAGCGGGGAG 3721 AGGCCACAGA TCACCATCAA TGAGATTAGT GAAATCACCA GCATCAAGAA GGAGGATGTC 3781 ATCTCCACTC TGCAGTACCT CAATCTCATC AACTACTACA AGGGCCAGTA CATCCTCACA 3841 CTGTCAGAGG ACATCGTGGA TGGCCATGAG CGGGCCATGC TCAAGCGGCT CCTGCGGATC 3901 GACTCCAAGT GTCTGCACTT CACTCCCAAG GACTGGAGCA AGAGGGGGAA GTGGggatcc 3961 GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT 4021 GACAAGtagc ggccgcGaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc 4081 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag 4141 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc 4201 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac 4261 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc 4321 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc 4381 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg 4441 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc 4501 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga 4561 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg 4621 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg 4681 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc 4741 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac 4801 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc 4861 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc 4921 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc 4981 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac 5041 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc 5101 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac 5161 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac 5221 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc 5281 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc 5341 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc 5401 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg 5461 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat 5521 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg 5581 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg 5641 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg 5701 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc 5761 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt 5821 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc 5881 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc 5941 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt 6001 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 6061 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 6121 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 6181 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 6241 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 6301 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 6361 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aATCATCGTC CTTTCCTtgg 6421 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 6481 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 6541 cgtctTcgcc ttcgccctca gacgagtcgg atctcccttt ggGccgcctc cccgcctggt 6601 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 6661 ggggggactg gaagggctaa ttcactccca acgaaGataa gatctgcttt ttgcttgtac 6721 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 6781 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 6841 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 6901 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 6961 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 7021 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 7081 catcaatgta tcttatcatg tctggctcta gctaTCCCGC CCCTAACTCC GCCCAtcccg 7141 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 7201 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 7261 tttggaggcc tagacttttg cagagaccaa attcgtaatc atgtcatagc tgtttcctgt 7321 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 7381 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 7441 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 7501 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 7561 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 7621 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 7681 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 7741 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 7801 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 7861 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 7921 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 7981 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 8041 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 8101 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 8161 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 8221 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 8281 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 8341 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 8401 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 8461 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 8521 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 8581 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 8641 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 8701 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 8761 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 8821 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 8881 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 8941 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 9001 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 9061 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 9121 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 9181 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 9241 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 9301 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 9361 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 9421 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 9481 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga 9541 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 9601 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 9661 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 9721 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct 9781 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 9841 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 9901 ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.