pCMV-Myh10-3×HA-Neo plasmid

pCMV-Myh10-3×HA-Neo plasmid

PVT91233

pCMV-Myh10-3×HA-Neo plasmid Information

Plasmid name: pCMV-Myh10(Rat)-3×HA-Neo
Alias: NM_031520.2;MCH-B; NMMHC-B; SMemb
Gene lenght: 5928bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Rat
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Myh10-3×HA-Neo plasmid Description

pCMV-Myh10-3×HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Myh10-3×HA-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCATGGC CCAGAGAACT 721 GGACTGGAGG ATCCCGAGAG GTATCTCTTT GTGGACAGGG CTGTCATCTA CAACCCTGCC 781 ACTCAAGCTG ACTGGACAGC TAAAAAGCTG GTGTGGATTC CATCTGAACG CCATGGTTTT 841 GAGGCAGCTA GTATTAAAGA AGAACGGGGC GATGAGGTTA TGGTGGAGCT GGCAGAGAAC 901 GGGAAGAAAG CGATGGTCAA CAAAGATGAC ATTCAGAAGA TGAACCCGCC GAAGTTCTCC 961 AAGGTGGAGG ACATGGCAGA GCTCACGTGC TTGAACGAAG CCTCTGTTTT ACACAATCTG 1021 AAGGATCGCT ACTATTCAGG ACTCATCTAT ACTTACTCTG GACTCTTCTG TGTGGTGATA 1081 AATCCCTACA AGAACCTTCC GATTTACTCT GAGAATATTA TTGAGATGTA CAGAGGGAAG 1141 AAGCGCCATG AGATGCCGCC ACACATCTAC GCCATATCAG AGTCTGCCTA CAGATGCATG 1201 CTTCAAGATC GTGAGGACCA GTCGATTCTT TGCACGGGTG AATCAGGTGC CGGGAAGACA 1261 GAAAATACAA AGAAAGTCAT TCAGTACCTT GCCCACGTTG CTTCTTCACA CAAAGGAAGA 1321 AAGGACCACA ATATTCCTGG GGAACTTGAA CGGCAGCTTT TACAAGCAAA TCCAATTCTG 1381 GAATCATTTG GAAATGCGAA GACTGTGAAA AATGATAACT CATCTCGCTT TGGCAAGTTT 1441 ATCCGGATCA ACTTTGATGT AACCGGCTAT ATTGTTGGGG CCAACATTGA AACATACCTT 1501 CTGGAAAAGT CCCGTGCTGT TCGTCAAGCT AAAGATGAAC GCACGTTTCA TATCTTTTAT 1561 CAGTTGCTCT CTGGAGCAGG GGAACACCTG AAGTCCGACT TACTCCTGGA AGGCTTTAAT 1621 AACTACAGAT TCCTCTCCAA CGGCTACATC CCTATTCCTG GACAGCAAGA CAAGGATAAC 1681 TTTCAGGAGA CCATGGAAGC CATGCACATA ATGGGCTTCT CTCACGAAGA GATCCTCTCA 1741 ATGCTTAAAG TCGTGTCTTC AGTGCTGCAG TTTGGAAACA TCTCTTTCAA AAAGGAGAGG 1801 AACACTGACC AAGCTTCCAT GCCCGAGAAC ACAGTTGCAC AGAAACTCTG CCACCTGCTT 1861 GGGATGAATG TGATGGAGTT CACTCGGGCT ATCCTCACGC CCAGGATCAA AGTTGGCCGA 1921 GATTATGTAC AGAAAGCCCA GACCAAAGAG CAGGCAGATT TTGCAGTGGA AGCATTGGCA 1981 AAGGCTACCT ATGAGCGGTT GTTTCGCTGG CTCGTTCACC GCATCAATAA GGCGCTGGAT 2041 AGGACCAAAC GCCAGGGAGC GTCTTTCATT GGGATCCTGG ATATTGCTGG TTTTGAAATT 2101 TTTGAGCTGA ACTCCTTTGA ACAACTATGC ATCAACTACA CCAATGAGAA GCTGCAGCAG 2161 CTGTTCAACC ACACCATGTT CATCCTGGAG CAGGAGGAGT ACCAGCGAGA GGGCATCGAG 2221 TGGAACTTCA TCGACTTTGG TCTCGACTTG CAGCCCTGCA TCGACCTGAT AGAGAGACCT 2281 GCCAATCCCC CTGGTGTGCT GGCCCTCCTG GATGAAGAAT GCTGGTTCCC CAAAGCCACA 2341 GACAAAACGT TTGTTGAGAA GCTGGTTCAG GAGCAAGGTT CCCACTCCAA GTTTCAGAAA 2401 CCACGGCAAC TGAAAGACAA AGCCGACTTC TGCATCATCC ACTATGCAGG GAAGGTGGAC 2461 TATAAGGCGG ATGAGTGGCT GATGAAGAAC ATGGACCCCC TGAATGACAA CGTGGCCACC 2521 CTCCTGCACC AGTCATCAGA CAGATTTGTC GCCGAGCTTT GGAAGGATGT GGACCGAATT 2581 GTAGGTCTGG ATCAAGTCAC TGGCATGACT GAGACAGCTT TTGGCTCTGC ATATAAAACC 2641 AAGAAGGGCA TGTTCCGCAC CGTCGGGCAG CTCTACAAAG AGTCTCTCAC CAAGCTCATG 2701 GCAACCCTCC GAAACACCAA CCCCAACTTC GTACGCTGCA TCATTCCAAA TCACGAGAAG 2761 CGGGCTGGAA AACTGGATCC ACATCTCGTG CTGGATCAGC TCCGCTGTAA CGGTGTCCTG 2821 GAAGGAATCA GGATCTGTCG CCAGGGATTC CCCAACCGGA TAGTTTTCCA GGAGTTCAGA 2881 CAGAGATATG AGATCCTAAC TCCCAACGCT ATTCCCAAAG GCTTCATGGA TGGCAAACAG 2941 GCCTGTGAGC GAATGATCCG AGCTTTAGAA CTGGACCCAA ACCTGTATAG AATTGGACAG 3001 AGCAAGATAT TTTTCCGAGC TGGAGTTCTG GCGCACTTAG AGGAAGAAAG AGATTTAAAA 3061 ATCACTGATA TAATCATCTT TTTCCAAGCT GTATGCAGAG GCTACCTAGC CAGAAAGGCC 3121 TTTGCCAAGA AACAGCAGCA GCTAAGTGCC TTAAAGGTCT TGCAGCGGAA CTGTGCGGCG 3181 TACCTGAAGC TGCGACACTG GCAGTGGTGG CGCGTCTTCA CAAAGGTGAA GCCTCTCCTC 3241 CAAGTGACCC GCCAGGAGGA GGAGCTCCAG GCTAAAGATG AGGAGCTGCT GAAGGTGAAA 3301 GAGAAGCAGA CAAAAGTGGA AGGGGAACTT GAGGAGATGG AACGGAAGCA CCAGCAGCTG 3361 CTGGAAGAGA AGAATATCCT GGCAGAACAG CTGCAGGCCG AGACTGAGCT CTTTGCTGAA 3421 GCAGAGGAGA TGAGAGCAAG ACTCGCTGCC AAGAAGCAGG AACTGGAGGA GATCCTCCAT 3481 GACCTGGAGT CCAGGGTGGA GGAGGAGGAA GAGCGAAACC AGATCCTGCA GAACGAGAAG 3541 AAGAAGATGC AAGCGCACAT TCAGGATCTA GAAGAGCAAC TGGATGAGGA GGAGGGGGCG 3601 CGGCAAAAGC TGCAGCTGGA GAAGGTGACC GCGGAGGCTA AAATCAAGAA GATGGAAGAG 3661 GAGGTTCTGC TTCTCGAGGA CCAGAATTCC AAATTTATCA AAGAAAAGAA ACTCATGGAA 3721 GATCGAATTG CTGAGTGTTC CTCTCAGCTG GCTGAAGAGG AAGAAAAGGC AAAAAACTTA 3781 GCCAAAATCA GGAATAAGCA AGAAGTGATG ATCTCGGATT TAGAAGAACG CTTGAAGAAG 3841 GAGGAGAAAA CACGGCAGGA ACTGGAAAAG GCCAAACGGA AACTGGACGG TGAAACAACT 3901 GATCTGCAGG ACCAGATCGC CGAGTTGCAA GCACAGGTCG ATGAGCTCAA AGTCCAGTTG 3961 ACCAAGAAGG AGGAGGAGCT TCAGGGGGCG CTGGCCAGAG GAGATGATGA GACGCTGCAC 4021 AAGAATAATG CGCTTAAAGT TGCGCGGGAG CTGCAGGCCC AAATCGCCGA GCTCCAGGAA 4081 GACTTTGAGT CTGAAAAGGC TTCAAGGAAC AAGGCTGAGA AACAAAAACG GGACCTGAGT 4141 GAGGAGCTGG AAGCTCTAAA GACAGAACTG GAGGACACCC TAGACACCAC AGCAGCTCAG 4201 CAGGAACTCC GCACAAAGCG GGAGCAGGAA GTAGCTGAGC TGAAGAAGGC TCTTGAGGAT 4261 GAAACTAAGA ACCACGAAGC TCAGATCCAG GACATGAGAC AGAGGCACGC CACGGCACTG 4321 GAGGAACTCT CTGAGCAACT GGAACAGGCT AAGAGGTTCA AAGCCAATCT GGAGAAGAAC 4381 AAACAGGGCC TGGAGACGGA CAACAAGGAG CTGGCGTGTG AGGTGAAGGT GCTGCAGCAA 4441 GTCAAGGCTG AGTCAGAGCA CAAGAGGAAG AAGCTCGATG CCCAGGTCCA GGAGCTCCAC 4501 GCCAAGGTGT CAGAAGGTGA CAGGCTCAGG GTAGAACTGG CCGAGAAAGC AAACAAGCTA 4561 CAGAATGAGC TGGATAATGT CTCAACCCTG CTGGAAGAAG CCGAGAAGAA AGGAATGAAG 4621 TTCGCTAAGG ACGCCGCAGG TCTCGAGTCT CAGCTACAGG ACACACAGGA GCTCCTTCAG 4681 GAGGAGACGC GGCAGAAACT GAACCTGAGC AGTCGGATCC GGCAGCTGGA GGAGGAGAAG 4741 AACAGCCTTC AGGAGCAGCA GGAGGAGGAA GAGGAGGCCA GGAAGAACTT GGAAAAGCAG 4801 GTGTTGGCCC TGCAGTCCCA GCTGGCTGAC ACCAAGAAGA AAGTGGACGA TGACCTGGGG 4861 ACAATTGAGG GTTTGGAAGA AGCCAAGAAG AAACTGCTTA AGGACGTGGA GGCACTGAGT 4921 CAGCGACTGG AGGAAAAGGT CCTAGCATAT GACAAGCTGG AGAAGACCAA GAACCGGCTG 4981 CAACAGGAGC TGGATGACCT GACGGTGGAC CTGGACCACC AGCGCCAGAT CGTCTCCAAC 5041 TTGGAGAAGA AACAGAAGAA GTTTGACCAG CTGTTGGCAG AAGAGAAGGG CATCTCTGCT 5101 CGCTATGCAG AAGAACGGGA CCGGGCTGAA GCTGAGGCCA GAGAGAAAGA AACCAAGGCG 5161 CTCTCCCTGG CGCGGGCCCT GGAGGAGGCC TTGGAGGCCA AGGAGGAATT CGAGAGGCAG 5221 AATAAGCAGC TTCGGGCAGA CATGGAAGAC CTCATGAGCT CGAAAGATGA TGTGGGGAAG 5281 AACGTCCACG AGCTGGAGAA GTCCAAGCGG GCCCTGGAGC AGCAGGTGGA GGAGATGAGG 5341 ACCCAGCTGG AGGAGCTGGA GGACGAGTTG CAGGCCACTG AGGACGCCAA ACTCCGCCTG 5401 GAAGTCAACA TGCAGGCCAT GAAGGCCCAG TTTGAGAGGG ACCTGCAAAC CAGAGATGAG 5461 CAGAATGAAG AGAAGAAGCG GCTGCTGCTC AAGCAGGTAC GGGAGCTCGA GGCAGAGCTG 5521 GAGGATGAGC GGAAGCAGCG GGCACTTGCT GTGGCTTCAA AGAAGAAGAT GGAGATAGAC 5581 CTGAAGGACC TGGAGGCTCA GATCGAGGCC GCCAACAAGG CCCGGGATGA AGTGATCAAG 5641 CAGCTTCGCA AGCTTCAGGC ACAGATGAAG GATTACCAGC GTGAACTAGA AGAAGCTCGA 5701 GCATCTAGAG ATGAGATTTT TGCTCAATCC AAAGAAAGTG AAAAGAAACT GAAGAGTCTA 5761 GAAGCAGAAA TCCTCCAGTT GCAAGAGGAG CTGGCCTCAT CTGAGCGAGC CCGCAGACAC 5821 GCAGAGCAGG AGCGAGATGA GCTGGCTGAC GAGATTGCCA ACAGCGCCTC TGGGAAGTCC 5881 GCTCTGCTGG ATGAGAAGCG GCGCCTGGAA GCCCGGATCG CGCAGCTGGA GGAGGAGCTA 5941 GAAGAGGAGC AGAGTAACAT GGAGCTGCTC AATGACCGCT TCCGCAAGAC CACGCTCCAG 6001 GTGGACACCC TGAACACAGA GTTGGCAGCG GAGCGCAGCG CTGCCCAGAA GAGCGACAAT 6061 GCCCGCCAGC AGCTGGAGCG ACAAAACAAG GAGCTGAAGG CCAAGCTGCA GGAGCTGGAG 6121 GGGGCGGTCA AGTCCAAGTT CAAAGCCACC ATCTCAGCCC TGGAAGCCAA GATTGGGCAG 6181 CTGGAGGAAC AGCTGGAGCA GGAAGCCAAG GAACGCGCAG CTGCCAACAA ACTAGTCCGT 6241 CGGACAGAGA AGAAACTGAA AGAGATCTTC ATGCAGGTCG AAGACGAGCG CCGGCATGCG 6301 GATCAGTATA AGGAGCAGAT GGAGAAAGCC AATGCCAGGA TGAAGCAGCT TAAACGCCAG 6361 CTGGAAGAAG CTGAGGAGGA GGCTACACGT GCCAATGCAT CTCGGCGTAA ACTCCAAAGG 6421 GAACTGGATG ATGCCACCGA GGCCAATGAG GGCCTGAGCC GTGAGGTCAG CACTCTCAAG 6481 AACCGGCTTA GGCGGGGCGG CCCAATCAGC TTTTCTTCAA GCCGATCTGG CCGGCGCCAG 6541 CTACACATTG AGGGGGCATC GCTAGAGCTG TCAGATGACG ACACAGAAAG CAAGACCAGT 6601 GATGTCAATG AGACACAGCC ACCCCAGTCG GAACTCGAGT ACCCATACGA CGTACCTGAT 6661 TATGCAACTT ATCCTTATGA TGTACCTGAT TATGCTAGCT ATCCTTATGA TGTCCCAGAC 6721 TACGCATAGT GAGGGCCCGA TATCTCGACA ATCAACCTCT GGATTACAAA ATTTGTGAAA 6781 GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA 6841 TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT 6901 CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT 6961 GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC 7021 TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC 7081 TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG 7141 GGAAGCTGAC GTCCTTTCCA TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA 7201 CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC 7261 TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC 7321 TTTGGGCCGC CTCCCCGCCT GGAAACGGGG GAGGCTAACT GAAACACGGA AGGAGACAAT 7381 ACCGGAAGGA ACCCGCGCTA TGACGGCAAT AAAAAGACAG AATAAAACGC ACGGGTGTTG 7441 GGTCGTTTGT TCATAAACGC GGGGTTCGGT CCCAGGGCTG GCACTCTGTC GATACCCCAC 7501 CGAGACCCCA TTGGGGCCAA TACGCCCGCG TTTCTTCCTT TTCCCCACCC CACCCCCCAA 7561 GTTCGGGTGA AGGCCCAGGG CTCGCAGCCA ACGTCGGGGC GGCAGGCCCT GCCATAGCAG 7621 ATCTGCGCAG CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAGCG 7681 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 7741 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 7801 TAAATCGGGG CATCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 7861 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 7921 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 7981 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGGGGATT TCGGCCTATT 8041 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTAATTCTGT GGAATGTGTG 8101 TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA 8161 TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT 8221 GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC 8281 GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT 8341 TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT 8401 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC 8461 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG 8521 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG 8581 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA 8641 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT 8701 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA 8761 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC 8821 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC 8881 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC 8941 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT 9001 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA 9061 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG 9121 CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA 9181 AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA 9241 TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG 9301 TTCGCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 9361 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 9421 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 9481 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 9541 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 9601 CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 9661 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 9721 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 9781 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 9841 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 9901 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 9961 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 10021 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 10081 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 10141 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 10201 TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC AGTTCGGTGT 10261 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 10321 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 10381 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 10441 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC 10501 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 10561 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 10621 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 10681 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 10741 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT 10801 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT 10861 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG 10921 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG 10981 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 11041 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG 11101 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG 11161 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT 11221 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA 11281 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 11341 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC 11401 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG 11461 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA 11521 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG 11581 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 11641 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 11701 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 11761 CATTTCCCCG AAAAGTGCCA CCTGACGTCG ACGGATCGGG AGATCTCCCG ATCCCCTATG 11821 GTCGACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATC TGCTCCCTGC 11881 TTGTGTGTTG GAGGTCGCTG AGTAGTGCGC GAGCAAAATT TAAGCTACAA CAAGGCAAGG 11941 CTTGACCGAC AATTGCATGA AGAATCTGCT TAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.