pLV3-CMV-HOXD11-3×FLAG-EF1a-Co

pLV3-CMV-HOXD11-3×FLAG-EF1a-CopGFP-Neo plasmid

PVT94713

pLV3-CMV-HOXD11-3×FLAG-EF1a-CopGFP-Neo plasmid Information

Plasmid name: pLV3-CMV-HOXD11(human)-3×FLAG-EF1a-CopGFP-Neo
Alias: NM_021192.3;HOX4; HOX4F
Gene lenght: 1014bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-HOXD11-3×FLAG-EF1a-CopGFP-Neo plasmid Description

pLV3-CMV-HOXD11-3×FLAG-EF1a-CopGFP-Neo is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-HOXD11-3×FLAG-EF1a-CopGFP-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatgaacga ctttgacgag tgcggccaga gcgcagccag 2341 catgtacctg ccgggctgcg cctactatgt ggccccgtct gacttcgcta gcaagccttc 2401 gttcctttcc caaccgtcgt cctgccagat gactttcccc tactcttcca acctggctcc 2461 gcacgtccag cccgtgcgcg aagtggcctt ccgcgactac ggcctggagc gcgccaagtg 2521 gccgtaccgc ggcggcggcg gcggcggcag cgcggggggc ggcagcagcg ggggcggccc 2581 cggcgggggc ggcggcggcg cggggggcta cgctccctac tacgcggcgg cggcggcggc 2641 ggctgcggcg gccgcggcgg ccgaggaggc ggccatgcaa cgcgagcttc tcccgcccgc 2701 gggccgccgg ccggacgtgc tcttcaaggc gcctgagccg gtgtgcgctg cgccggggcc 2761 gccgcacggc cccgcgggcg ccgcctccaa cttctacagc gcggtgggcc gcaatggcat 2821 cttgccacag ggcttcgacc agttctacga ggcagcgccc gggcccccgt tcgccgggcc 2881 gcagcccccg ccgccacccg cgccgccaca gcccgagggc gcagccgaca agggcgaccc 2941 caggaccggg gctggtggcg gcgggggcag tccctgcacc aaggcgaccc ctggctcgga 3001 gcccaagggg gcagcagaag gcagcggtgg cgacggcgag ggccccccgg gagaggcggg 3061 ggccgagaag agcagcagcg cagttgcccc ccagcggtcc cggaaaaagc gctgtcccta 3121 taccaagtac cagatccgcg aactggaacg cgagtttttc tttaacgtgt acataaacaa 3181 agagaaaaga cttcaactct ctcggatgct caacctcact gaccggcaag tcaaaatctg 3241 gttccagaat cgcaggatga aagaaaagaa actgaacaga gaccgtctgc agtatttcac 3301 tggaaacccc ttatttggat ccGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA 3361 CATCGACTAC AAGGATGACG ATGACAAGTA gcggccgcGa aggatctgcg atcgctccgg 3421 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 3481 cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 3541 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 3601 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc 3661 tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag 3721 tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag 3781 tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact 3841 cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt 3901 ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat 3961 ggagagcgac gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct 4021 gaacggcgtg gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat 4081 gaccaacaag atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca 4141 cgtgatgggc tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt 4201 cctgcacgcc atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg 4261 cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt 4321 caaggtggtg ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg 4381 cagcaacgcc accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt 4441 cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat 4501 gcacttcaag agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt 4561 ccgccgcgtg gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc 4621 cttcaagacc cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga 4681 cggcaccgcc ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg 4741 cggtgacgtg gaggagaatc ccggccctGC CACCatggct gaacaagatg gattgcacgc 4801 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat 4861 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt 4921 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg 4981 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag 5041 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc 5101 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc 5161 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga 5221 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga 5281 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg 5341 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg 5401 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc 5461 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc 5521 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgaG TCGACAATCA 5581 ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT 5641 TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC 5701 TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC 5761 CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG 5821 GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC 5881 CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG 5941 CACTGACAAT TCCGTGGTGT TGTCGGGGAA ATCATCGTCC TTTCCTTGGC TGCTCGCCTG 6001 TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC 6061 AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT 6121 TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCggtacc tttaagacca 6181 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa 6241 gggctaattc actcccaacg aaGataagat ctgctttttg cttgtactgg gtctctctgg 6301 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 6361 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 6421 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca 6481 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg 6541 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 6601 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 6661 tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc 6721 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 6781 tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa 6841 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 6901 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 6961 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 7021 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 7081 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 7141 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 7201 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 7261 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 7321 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 7381 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 7441 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 7501 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 7561 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 7621 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 7681 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 7741 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 7801 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 7861 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 7921 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 7981 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 8041 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 8101 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 8161 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 8221 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 8281 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 8341 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 8401 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 8461 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 8521 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 8581 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 8641 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 8701 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 8761 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 8821 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 8881 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 8941 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 9001 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca 9061 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc 9121 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 9181 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga 9241 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat 9301 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc 9361 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt 9421 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.