pLV3-CMV-HDAC3-3×FLAG-CopGFP-P

pLV3-CMV-HDAC3-3×FLAG-CopGFP-Puro plasmid

PVT94707

pLV3-CMV-HDAC3-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-HDAC3(human)-3×FLAG-CopGFP-Puro
Alias: NM_003883.4;HD3; KDAC3; RPD3; RPD3-2
Gene lenght: 1284bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-HDAC3-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-HDAC3-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-HDAC3-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg ccaagaccgt ggcctatttc 2341 tacgaccccg acgtgggcaa cttccactac ggagctggac accctatgaa gccccatcgc 2401 ctggcattga cccatagcct ggtcctgcat tacggtctct ataagaagat gatcgtcttc 2461 aagccatacc aggcctccca acatgacatg tgccgcttcc actccgagga ctacattgac 2521 ttcctgcaga gagtcagccc caccaatatg caaggcttca ccaagagtct taatgccttc 2581 aacgtaggcg atgactgccc agtgtttccc gggctctttg agttctgctc gcgttacaca 2641 ggcgcatctc tgcaaggagc aacccagctg aacaacaaga tctgtgatat tgccattaac 2701 tgggctggtg gtctgcacca tgccaagaag tttgaggcct ctggcttctg ctatgtcaac 2761 gacattgtga ttggcatcct ggagctgctc aagtaccacc ctcgggtgct ctacattgac 2821 attgacatcc accatggtga cggggttcaa gaagctttct acctcactga ccgggtcatg 2881 acggtgtcct tccacaaata cggaaattac ttcttccctg gcacaggtga catgtatgaa 2941 gtcggggcag agagtggccg ctactactgt ctgaacgtgc ccctgcggga tggcattgat 3001 gaccagagtt acaagcacct tttccagccg gttatcaacc aggtagtgga cttctaccaa 3061 cccacgtgca ttgtgctcca gtgtggagct gactctctgg gctgtgatcg attgggctgc 3121 tttaacctca gcatccgagg gcatggggaa tgcgttgaat atgtcaagag cttcaatatc 3181 cctctactcg tgctgggtgg tggtggttat actgtccgaa atgttgcccg ctgctggaca 3241 tatgagacat cgctgctggt agaagaggcc attagtgagg agcttcccta tagtgaatac 3301 ttcgagtact ttgccccaga cttcacactt catccagatg tcagcacccg catcgagaat 3361 cagaactcac gccagtatct ggaccagatc cgccagacaa tctttgaaaa cctgaagatg 3421 ctgaaccatg cacctagtgt ccagattcat gacgtgcctg cagacctcct gacctatgac 3481 aggactgatg aggctgatgc agaggagagg ggtcctgagg agaactatag caggccagag 3541 gcacccaatg agttctatga tggagaccat gacaatgaca aggaaagcga tgtggagatt 3601 ggatccGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT 3661 GACGATGACA AGtagcggcc gcGaaggatc tgcgatcgct ccggtgcccg tcagtgggca 3721 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt 3781 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt 3841 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt 3901 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca 3961 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct 4021 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg 4081 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg 4141 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt 4201 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc 4261 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc 4321 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag 4381 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc 4441 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac 4501 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg 4561 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc 4621 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg 4681 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc 4741 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc 4801 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag 4861 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc 4921 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc 4981 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag 5041 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc 5101 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc 5161 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc 5221 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg 5281 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc 5341 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac 5401 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc 5461 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg 5521 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc 5581 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag 5641 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 5701 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 5761 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 5821 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 5881 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 5941 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 6001 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaATC ATCGTCCTTT 6061 CCTtggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 6121 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 6181 cttccgcgtc tTcgccttcg ccctcagacg agtcggatct ccctttggGc cgcctccccg 6241 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 6301 agaaaagggg ggactggaag ggctaattca ctcccaacga aGataagatc tgctttttgc 6361 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 6421 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 6481 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 6541 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 6601 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa 6661 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 6721 caaactcatc aatgtatctt atcatgtctg gctctagcta TCCCGCCCCT AACTCCGCCC 6781 Atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 6841 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 6901 ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt 6961 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 7021 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 7081 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 7141 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 7201 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 7261 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 7321 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 7381 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 7441 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 7501 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 7561 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 7621 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 7681 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 7741 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 7801 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 7861 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 7921 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 7981 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 8041 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 8101 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 8161 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 8221 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 8281 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 8341 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 8401 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 8461 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 8521 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 8581 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 8641 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 8701 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 8761 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 8821 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 8881 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 8941 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 9001 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 9061 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 9121 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt 9181 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 9241 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 9301 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg 9361 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt 9421 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 9481 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 9541 acgacgttgt aaaacgacgg ccagtgccaa gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.