pLV3-CMV-EGFP-Erbb2-3×FLAG-Bla

pLV3-CMV-EGFP-Erbb2-3×FLAG-Blast plasmid

PVT88986

pLV3-CMV-EGFP-Erbb2-3×FLAG-Blast plasmid Information

Plasmid name: pLV3-CMV-EGFP-Erbb2(rat)-3×FLAG-Blast
Alias: NM_017003.3;HER2/neu; neu
Gene lenght: 3777bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Rat
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-EGFP-Erbb2-3×FLAG-Blast plasmid Description

pLV3-CMV-EGFP-Erbb2-3×FLAG-Blast It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-EGFP-Erbb2-3×FLAG-Blast plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catgatcatc atggagctgg cggcctggtg ccgctggggg 2341 ttcctcctcg ccctcctgcc ccccggaatc gcgggcaccc aagtgtgtac cggcacagac 2401 atgaagttgc ggctccctgc cagtcctgag acccacctgg acatgctccg ccacctgtac 2461 cagggctgtc aggtagtgca gggcaacttg gagcttacct acgtgcctgc caatgccagc 2521 ctctcattcc tgcaggacat ccaggaagtt cagggttaca tgctcatcgc tcacaaccag 2581 gtgaagcgcg tcccactgca aaggctgcgc atcgtgagag ggacccagct ctttgaggac 2641 aagtatgccc tggctgtgct agacaaccga gatcctcagg acaatgtcgc cgcctccacc 2701 ccaggcagaa ccccagaggg gctgcgggag ctgcagcttc gaagtctcac agagatcctg 2761 aagggaggag ttttgatccg tgggaaccct cagctctgct accaggacat ggttttgtgg 2821 aaggacgtct tccgcaagaa taaccaactg gctcctgtcg atatagacac caatcgttcc 2881 cgggcctgtc caccttgtgc ccccgcctgc aaagacaatc actgttgggg tgagagtccg 2941 gaagactgtc agatcttgac tggcaccatc tgtaccagtg gttgtgcccg gtgcaagggc 3001 cggctgccca ctgactgctg ccatgagcag tgtgccgcag gctgcacggg ccccaagcat 3061 tctgactgcc tggcctgcct ccacttcaat catagtggta tctgtgagct gcactgccca 3121 gccctcgtca cctacaacac agacaccttt gagtccatgc acaaccctga gggtcgctac 3181 acctttggtg ccagctgcgt gaccacctgc ccctacaact acctgtctac ggaagtggga 3241 tcctgcactc tggtgtgtcc cccgaataac caagaggtca cagctgagga cggaacacag 3301 cgttgtgaga aatgcagcaa gccctgtgct cgagtgtgct atggtctggg catggagcac 3361 cttcgagggg cgagggccat caccagtgac aatgtccagg agtttgatgg ctgcaagaag 3421 atctttggga gcctggcatt tttgccggag agctttgatg gggacccctc ctccggcatt 3481 gctccgctga ggcctgagca gctccaagtg ttcgaaaccc tggaggagat cacaggttac 3541 ctgtacatct cagcatggcc agacagtctc cgtgacctca gtgtcttcca gaaccttcga 3601 atcattcggg gacggattct ccacgatggc gcgtactcat tgacactgca aggcctgggg 3661 atccactcgc tggggctgcg ctcactgcgg gagctgggca gtggattggc tctgattcac 3721 cgcaacgccc atctctgctt tgtacacact gtaccttggg accagctctt ccggaaccca 3781 catcaggccc tgctccacag tgggaaccgg ccggaagagg attgtggtct cgagggcttg 3841 gtctgtaact cactgtgtgc ccacgggcac tgctgggggc cagggcccac ccagtgtgtc 3901 aactgcagtc atttccttcg gggccaggag tgtgtggagg agtgccgagt atggaagggg 3961 ctcccccggg agtatgtgag tgacaagcgc tgtctgccgt gtcaccccga gtgtcagcct 4021 caaaacagct cagagacctg ctttggatcg gaggctgatc agtgtgcagc ctgcgcccac 4081 tacaaggact cgtcctcctg tgtggctcgc tgccccagtg gtgtgaaacc ggacctctcc 4141 tacatgccca tctggaagta cccggatgag gagggcatat gccagccgtg ccccatcaac 4201 tgcacccact cctgtgtgga tctggatgaa cgaggctgcc cagcagagca gagagccagc 4261 ccggtgacat tcatcattgc aactgtagtg ggcgtcctgc tgttcctgat cttagtggtg 4321 gtcgttggaa tcctaatcaa acgaaggaga cagaagatcc ggaagtatac gatgcgtagg 4381 ctgctgcagg aaactgagtt agtggagccg ctgacgccca gcggagcaat gcccaaccag 4441 gctcagatgc ggatcctaaa agagacggag ctaaggaagg tgaaggtgct tggatcagga 4501 gcttttggca ctgtctacaa gggcatctgg atcccagatg gggagaatgt gaaaatcccc 4561 gtggctatca aggtgttgag agaaaacaca tctcctaaag ccaacaaaga aattctagat 4621 gaagcgtatg tgatggctgg tgtgggttct ccgtatgtgt cccgcctcct gggcatctgc 4681 ctgacatcca cagtacagct ggtgacacag cttatgccct acggctgcct tctggaccat 4741 gtccgagaac accgaggtcg cctaggctcc caggacctgc tcaactggtg tgttcagatt 4801 gccaagggga tgagctacct ggaggacgtg cggcttgtac acagggacct ggctgcccgg 4861 aatgtgctag tcaagagtcc caaccacgtc aagattacag atttcgggct ggctcggctg 4921 ctggacattg atgagacaga gtaccatgca gatgggggca aggtgcccat caaatggatg 4981 gcattggaat ctattctcag acgccggttc acccatcaga gtgatgtgtg gagctatgga 5041 gtgactgtgt gggagctgat gacttttggg gccaaacctt acgatggaat cccagcccgg 5101 gagatccctg atttgctgga gaagggagaa cgcctacctc agcctccaat ctgcaccatt 5161 gatgtctaca tgattatggt caaatgttgg atgattgact ctgaatgtcg cccgagattc 5221 cgggagttgg tgtcagaatt ttcacgtatg gcgagggacc cccagcgttt tgtggtcatc 5281 cagaacgagg acttgggccc atccagcccc atggacagta ccttctaccg ttcactgctg 5341 gaagatgatg acatgggtga cctggtagac gctgaagagt atctggtgcc ccagcaggga 5401 ttcttctccc cggaccctac cccaggcact gggagcacag cccatagaag gcaccgcagc 5461 tcgtccacca ggagtggagg tggtgagctg acactgggcc tggagccctc ggaagaaggg 5521 ccccccagat ctccactggc tccctcggaa ggggctggct ccgatgtgtt tgatggtgac 5581 ctggcaatgg gggtaaccaa agggctgcag agcctctctc cacatgacct cagccctcta 5641 cagcggtaca gcgaggaccc cacattacct ctgccccccg agactgatgg ctatgttgct 5701 cccctggcct gcagccccca gcccgagtat gtgaaccaat cagaggttca gcctcagcct 5761 cctttaaccc cagagggtcc tctgcctcct gtccggcctg ctggtgctac tctagaaaga 5821 cccaagactc tctctcctgg gaagaatggg gttgtcaaag acgtttttgc cttcgggggt 5881 gctgtggaga accctgaata cttagtaccg agagaaggca ctgcctctcc gccccaccct 5941 tctcctgcct tcagcccagc ctttgacaac ctctattact gggaccagaa ctcatcggag 6001 caggggcctc caccaagtaa ctttgaaggg acccccactg cagagaaccc tgagtaccta 6061 ggcctggatg tacctgtagg tggaggcgga tcaggaggcg gtggatctAT GGTGAGCAAG 6121 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC 6181 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC 6241 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC 6301 CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC 6361 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC 6421 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC 6481 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC 6541 AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG 6601 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG 6661 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC 6721 CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC 6781 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGGGATC CGACTACAAA 6841 GACCATGACG GTGATTATAA AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGtaG 6901 CGGCCGCGAA GGATCTGCGA TCGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC 6961 ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA CGGGTGCCTA GAGAAGGTGG 7021 CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG 7081 GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC 7141 GCCAGAACAC AGCTGAAGCT TCGAGGGGCT CGCATCTCTC CTTCACGCGC CCGCCGCCCT 7201 ACCTGAGGCC GCCATCCACG CCGGTTGAGT CGCGTTCTGC CGCCTCCCGC CTGTGGTGCC 7261 TCCTGAACTG CGTCCGCCGT CTAGGTAAGT TTAAAGCTCA GGTCGAGACC GGGCCTTTGT 7321 CCGGCGCTCC CTTGGAGCCT ACCTAGACTC AGCCGGCTCT CCACGCTTTG CCTGACCCTG 7381 CTTGCTCAAC TCTACGTCTT TGTTTCGTTT TCTGTTCTGC GCCGTTACAG ATCCAAGCTG 7441 TGACCGGCGC CTACGCTAGG CCACCATGGC CAAGCCTTTG TCTCAAGAAG AATCCACCCT 7501 CATTGAAAGA GCAACGGCTA CAATCAACAG CATCCCCATC TCTGAAGACT ACAGCGTCGC 7561 CAGCGCAGCT CTCTCTAGCG ACGGCCGCAT CTTCACTGGT GTCAATGTAT ATCATTTTAC 7621 TGGGGGACCT TGTGCAGAAC TCGTGGTGCT GGGCACTGCT GCTGCTGCGG CAGCTGGCAA 7681 CCTGACTTGT ATCGTCGCGA TCGGAAATGA GAACAGGGGC ATCTTGAGCC CCTGCGGACG 7741 GTGCCGACAG GTGCTTCTCG ATCTGCATCC TGGGATCAAA GCCATAGTGA AGGACAGTGA 7801 TGGACAGCCG ACGGCAGTTG GGATTCGTGA ATTGCTGCCC TCTGGTTATG TGTGGGAGGG 7861 CTAAGTCGAC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA 7921 CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT 7981 TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA 8041 TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC 8101 AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT 8161 CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG 8221 GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAATCAT CGTCCTTTCC 8281 TTGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC 8341 TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT 8401 TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCC 8461 TGGTACCTTT AAGACCAATG ACTTACAAGG CAGCTGTAGA TCTTAGCCAC TTTTTAAAAG 8521 AAAAGGGGGG ACTGGAAGGG CTAATTCACT CCCAACGAAA ATAAGATCTG CTTTTTGCTT 8581 GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC TAACTAGGGA 8641 ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG TGTGCCCGTC 8701 TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG TGGAAAATCT 8761 CTAGCAGTAG TAGTTCATGT CATCTTATTA TTCAGTATTT ATAACTTGCA AAGAAATGAA 8821 TATCAGAGAG TGAGAGGAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 8881 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 8941 AACTCATCAA TGTATCTTAT CATGTCTGGC TCTAGCTATC CCGCCCCTAA CTCCGCCCAG 9001 TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC 9061 CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGACTT 9121 TTGCAGAGAC GGCCCAAATT CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA 9181 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC 9241 CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG 9301 AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG 9361 TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG 9421 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 9481 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 9541 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 9601 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 9661 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 9721 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 9781 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 9841 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 9901 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 9961 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT 10021 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 10081 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 10141 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 10201 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 10261 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 10321 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 10381 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 10441 AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 10501 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 10561 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 10621 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 10681 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 10741 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 10801 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 10861 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 10921 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 10981 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 11041 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 11101 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 11161 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 11221 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 11281 ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA 11341 TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA 11401 CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG 11461 CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA 11521 TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG 11581 ATGCGTAAGG AGAAAATACC GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG 11641 GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC 11701 TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC 11761 GGCCAGTGCC AAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.