pCMV-ATM-8×His-Neo plasmid

pCMV-ATM-8×His-Neo plasmid

PVT94700

pCMV-ATM-8×His-Neo plasmid Information

Plasmid name: pCMV-ATM(human)-8×His-Neo
Alias: NM_001351834.2;AT1; ATA; ATC; ATD; ATDC; ATE; TEL1; TELO1
Gene lenght: 9168bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): BL21(DE3)
Growth Temperature: 30°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: Low
Induction:

pCMV-ATM-8×His-Neo plasmid Description

pCMV-ATM-8×His-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-ATM-8×His-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatgag tctagtactt 721 aatgatctgc ttatctgctg ccgtcaacta gaacatgata gagctacaga acgaaagaaa 781 gaagttgaga aatttaagcg cctgattcga gatcctgaaa caattaaaca tctagatcgg 841 cattcagatt ccaaacaagg aaaatatttg aattgggatg ctgtttttag atttttacag 901 aaatatattc agaaagaaac agaatgtctg agaatagcaa aaccaaatgt atcagcctca 961 acacaagcct ccaggcagaa aaagatgcag gaaatcagta gtttggtcaa atacttcatc 1021 aaatgtgcaa acagaagagc acctaggcta aaatgtcaag aactcttaaa ttatatcatg 1081 gatacagtga aagattcatc taatggtgct atttacggag ctgattgtag caacatacta 1141 ctcaaagaca ttctttctgt gagaaaatac tggtgtgaaa tatctcagca acagtggtta 1201 gaattgttct ctgtgtactt caggctctat ctgaaacctt cacaagatgt tcatagagtt 1261 ttagtggcta gaataattca tgctgttacc aaaggatgct gttctcagac tgacggatta 1321 aattccaaat ttttggactt tttttccaag gctattcagt gtgcgagaca agaaaagagc 1381 tcttcaggtc taaatcatat cttagcagct cttactatct tcctcaagac tttggctgtc 1441 aactttcgaa ttcgagtgtg tgaattagga gatgaaattc ttcccacttt gctttatatt 1501 tggactcaac ataggcttaa tgattcttta aaagaagtca ttattgaatt atttcaactg 1561 caaatttata tccatcatcc gaaaggagcc aaaacccaag aaaaaggtgc ttatgaatca 1621 acaaaatgga gaagtatttt atacaactta tatgatctgc tagtgaatga gataagtcat 1681 ataggaagta gaggaaagta ttcttcagga tttcgtaata ttgccgtcaa agaaaatttg 1741 attgaattga tggcagatat ctgtcaccag gtttttaatg aagataccag atccttggag 1801 atttctcaat cttacactac tacacaaaga gaatctagtg attacagtgt cccttgcaaa 1861 aggaagaaaa tagaactagg ctgggaagta ataaaagatc accttcagaa gtcacagaat 1921 gattttgatc ttgtgccttg gctacagatt gcaacccaat taatatcaaa gtatcctgca 1981 agtttaccta actgtgagct gtctccatta ctgatgatac tatctcagct tctaccccaa 2041 cagcgacatg gggaacgtac accatatgtg ttacgatgcc ttacggaagt tgcattgtgt 2101 caagacaaga ggtcaaacct agaaagctca caaaagtcag atttattaaa actctggaat 2161 aaaatttggt gtattacctt tcgtggtata agttctgagc aaatacaagc tgaaaacttt 2221 ggcttacttg gagccataat tcagggtagt ttagttgagg ttgacagaga attctggaag 2281 ttatttactg ggtcagcctg cagaccttca tgtcctgcag tatgctgttt gactttggca 2341 ctgaccacca gtatagttcc aggaacggta aaaatgggaa tagagcaaaa tatgtgtgaa 2401 gtaaatagaa gcttttcttt aaaggaatca ataatgaaat ggctcttatt ctatcagtta 2461 gagggtgact tagaaaatag cacagaagtg cctccaattc ttcacagtaa ttttcctcat 2521 cttgtactgg agaaaattct tgtgagtctc actatgaaaa actgtaaagc tgcaatgaat 2581 tttttccaaa gcgtgccaga atgtgaacac caccaaaaag ataaagaaga actttcattc 2641 tcagaagtag aagaactatt tcttcagaca acttttgaca agatggactt tttaaccatt 2701 gtgagagaat gtggtataga aaagcaccag tccagtattg gcttctctgt ccaccagaat 2761 ctcaaggaat cactggatcg ctgtcttctg ggattatcag aacagcttct gaataattac 2821 tcatctgaga ttacaaattc agaaactctt gtccggtgtt cacgtctttt ggtgggtgtc 2881 cttggctgct actgttacat gggtgtaata gctgaagagg aagcatataa gtcagaatta 2941 ttccagaaag ccaagtctct aatgcaatgt gcaggagaaa gtatcactct gtttaaaaat 3001 aagacaaatg aggaattcag aattggttcc ttgagaaata tgatgcagct atgtacacgt 3061 tgcttgagca actgtaccaa gaagagtcca aataagattg catctggctt tttcctgcga 3121 ttgttaacat caaagctaat gaatgacatt gcagatattt gtaaaagttt agcatccttc 3181 atcaaaaagc catttgaccg tggagaagta gaatcaatgg aagatgatac taatggaaat 3241 ctaatggagg tggaggatca gtcatccatg aatctattta acgattaccc tgatagtagt 3301 gttagtgatg caaacgaacc tggagagagc caaagtacca taggtgccat taatccttta 3361 gctgaagaat atctgtcaaa gcaagatcta cttttcttag acatgctcaa gttcttgtgt 3421 ttgtgtgtaa ctactgctca gaccaatact gtgtccttta gggcagctga tattcggagg 3481 aaattgttaa tgttaattga ttctagcacg ctagaaccta ccaaatccct ccacctgcat 3541 atgtatctaa tgcttttaaa ggagcttcct ggagaagagt accccttgcc aatggaagat 3601 gttcttgaac ttctgaaacc actatccaat gtgtgttctt tgtatcgtcg tgaccaagat 3661 gtttgtaaaa ctattttaaa ccatgtcctt catgtagtga aaaacctagg tcaaagcaat 3721 atggactctg agaacacaag ggatgctcaa ggacagtttc ttacagtaat tggagcattt 3781 tggcatctaa caaaggagag gaaatatata ttctctgtaa gaatggccct agtaaattgc 3841 cttaaaactt tgcttgaggc tgatccttat tcaaaatggg ccattcttaa tgtaatggga 3901 aaagactttc ctgtaaatga agtatttaca caatttcttg ctgacaatca tcaccaagtt 3961 cgcatgttgg ctgcagagtc aatcaataga ttgttccagg acacgaaggg agattcttcc 4021 aggttactga aagcacttcc tttgaagctt cagcaaacag cttttgaaaa tgcatacttg 4081 aaagctcagg aaggaatgag agaaatgtcc catagtgctg agaaccctga aactttggat 4141 gaaatttata atagaaaatc tgttttactg acgttgatag ctgtggtttt atcctgtagc 4201 cctatctgcg aaaaacaggc tttgtttgcc ctgtgtaaat ctgtgaaaga gaatggatta 4261 gaacctcacc ttgtgaaaaa ggttttagag aaagtttctg aaacttttgg atatagacgt 4321 ttagaagact ttatggcatc tcatttagat tatctggttt tggaatggct aaatcttcaa 4381 gatactgaat acaacttatc ttcttttcct tttattttat taaactacac aaatattgag 4441 gatttctata gatcttgtta taaggttttg attccacatc tggtgattag aagtcatttt 4501 gatgaggtga agtccattgc taatcagatt caagaggact ggaaaagtct tctaacagac 4561 tgctttccaa agattcttgt aaatattctt ccttattttg cctatgaggg taccagagac 4621 agtgggatgg cacagcaaag agagactgct accaaggtct atgatatgct taaaagtgaa 4681 aacttattgg gaaaacagat tgatcactta ttcattagta atttaccaga gattgtggtg 4741 gagttattga tgacgttaca tgagccagca aattctagtg ccagtcagag cactgacctc 4801 tgtgactttt caggggattt ggatcctgct cctaatccac ctcattttcc atcgcatgtg 4861 attaaagcaa catttgccta tatcagcaat tgtcataaaa ccaagttaaa aagcatttta 4921 gaaattcttt ccaaaagccc tgattcctat cagaaaattc ttcttgccat atgtgagcaa 4981 gcagctgaaa caaataatgt ttataagaag cacagaattc ttaaaatata tcacctgttt 5041 gttagtttat tactgaaaga tataaaaagt ggcttaggag gagcttgggc ctttgttctt 5101 cgagacgtta tttatacttt gattcactat atcaaccaaa ggccttcttg tatcatggat 5161 gtgtcattac gtagcttctc cctttgttgt gacttattaa gtcaggtttg ccagacagcc 5221 gtgacttact gtaaggatgc tctagaaaac catcttcatg ttattgttgg tacacttata 5281 ccccttgtgt atgagcaggt ggaggttcag aaacaggtat tggacttgtt gaaatactta 5341 gtgatagata acaaggataa tgaaaacctc tatatcacga ttaagctttt agatcctttt 5401 cctgaccatg ttgtttttaa ggatttgcgt attactcagc aaaaaatcaa atacagtaga 5461 ggaccctttt cactcttgga ggaaattaac cattttctct cagtaagtgt ttatgatgca 5521 cttccattga caagacttga aggactaaag gatcttcgaa gacaactgga actacataaa 5581 gatcagatgg tggacattat gagagcttct caggataatc cgcaagatgg gattatggtg 5641 aaactagttg tcaatttgtt gcagttatcc aagatggcaa taaaccacac tggtgaaaaa 5701 gaagttctag aggctgttgg aagctgcttg ggagaagtgg gtcctataga tttctctacc 5761 atagctatac aacatagtaa agatgcatct tataccaagg cccttaagtt atttgaagat 5821 aaagaacttc agtggacctt cataatgctg acctacctga ataacacact ggtagaagat 5881 tgtgtcaaag ttcgatcagc agctgttacc tgtttgaaaa acattttagc cacaaagact 5941 ggacatagtt tctgggagat ttataagatg acaacagatc caatgctggc ctatctacag 6001 ccttttagaa catcaagaaa aaagttttta gaagtaccca gatttgacaa agaaaaccct 6061 tttgaaggcc tggatgatat aaatctgtgg attcctctaa gtgaaaatca tgacatttgg 6121 ataaagacac tgacttgtgc ttttttggac agtggaggca caaaatgtga aattcttcaa 6181 ttattaaagc caatgtgtga agtgaaaact gacttttgtc agactgtact tccatacttg 6241 attcatgata ttttactcca agatacaaat gaatcatgga gaaatctgct ttctacacat 6301 gttcagggat ttttcaccag ctgtcttcga cacttctcgc aaacgagccg atccacaacc 6361 cctgcaaact tggattcaga gtcagagcac tttttccgat gctgtttgga taaaaaatca 6421 caaagaacaa tgcttgctgt tgtggactac atgagaagac aaaagagacc ttcttcagga 6481 acaattttta atgatgcttt ctggctggat ttaaattatc tagaagttgc caaggtagct 6541 cagtcttgtg ctgctcactt tacagcttta ctctatgcag aaatctatgc agataagaaa 6601 agtatggatg atcaagagaa aagaagtctt gcatttgaag aaggaagcca gagtacaact 6661 atttctagct tgagtgaaaa aagtaaagaa gaaactggaa taagtttaca ggatcttctc 6721 ttagaaatct acagaagtat aggggagcca gatagtttgt atggctgtgg tggagggaag 6781 atgttacaac ccattactag actacgaaca tatgaacacg aagcaatgtg gggcaaagcc 6841 ctagtaacat atgacctcga aacagcaatc ccctcatcaa cacgccaggc aggaatcatt 6901 caggccttgc agaatttggg actctgccat attctttccg tctatttaaa aggattggat 6961 tatgaaaata aagactggtg tcctgaacta gaagaacttc attaccaagc agcatggagg 7021 aatatgcagt gggaccattg cacttccgtc agcaaagaag tagaaggaac cagttaccat 7081 gaatcattgt acaatgctct acaatctcta agagacagag aattctctac attttatgaa 7141 agtctcaaat atgccagagt aaaagaagtg gaagagatgt gtaagcgcag ccttgagtct 7201 gtgtattcgc tctatcccac acttagcagg ttgcaggcca ttggagagct ggaaagcatt 7261 ggggagcttt tctcaagatc agtcacacat agacaactct ctgaagtata tattaagtgg 7321 cagaaacact cccagcttct caaggacagt gattttagtt ttcaggagcc tatcatggct 7381 ctacgcacag tcattttgga gatcctgatg gaaaaggaaa tggacaactc acaaagagaa 7441 tgtattaagg acattctcac caaacacctt gtagaactct ctatactggc cagaactttc 7501 aagaacactc agctccctga aagggcaata tttcaaatta aacagtacaa ttcagttagc 7561 tgtggagtct ctgagtggca gctggaagaa gcacaagtat tctgggcaaa aaaggagcag 7621 agtcttgccc tgagtattct caagcaaatg atcaagaagt tggatgccag ctgtgcagcg 7681 aacaatccca gcctaaaact tacatacaca gaatgtctga gggtttgtgg caactggtta 7741 gcagaaacgt gcttagaaaa tcctgcggtc atcatgcaga cctatctaga aaaggcagta 7801 gaagttgctg gaaattatga tggagaaagt agtgatgagc taagaaatgg aaaaatgaag 7861 gcatttctct cattagcccg gttttcagat actcaatacc aaagaattga aaactacatg 7921 aaatcatcgg aatttgaaaa caagcaagct ctcctgaaaa gagccaaaga ggaagtaggt 7981 ctccttaggg aacataaaat tcagacaaac agatacacag taaaggttca gcgagagctg 8041 gagttggatg aattagccct gcgtgcactg aaagaggatc gtaaacgctt cttatgtaaa 8101 gcagttgaaa attatatcaa ctgcttatta agtggagaag aacatgatat gtgggtattc 8161 cgactttgtt ccctctggct tgaaaattct ggagtttctg aagtcaatgg catgatgaag 8221 agagacggaa tgaagattcc aacatataaa tttttgcctc ttatgtacca attggctgct 8281 agaatgggga ccaagatgat gggaggccta ggatttcatg aagtcctcaa taatctaatc 8341 tctagaattt caatggatca cccccatcac actttgttta ttatactggc cttagcaaat 8401 gcaaacagag atgaatttct gactaaacca gaggtagcca gaagaagcag aataactaaa 8461 aatgtgccta aacaaagctc tcagcttgat gaggatcgaa cagaggctgc aaatagaata 8521 atatgtacta tcagaagtag gagacctcag atggtcagaa gtgttgaggc actttgtgat 8581 gcttatatta tattagcaaa cttagatgcc actcagtgga agactcagag aaaaggcata 8641 aatattccag cagaccagcc aattactaaa cttaagaatt tagaagatgt tgttgtccct 8701 actatggaaa ttaaggtgga ccacacagga gaatatggaa atctggtgac tatacagtca 8761 tttaaagcag aatttcgctt agcaggaggt gtaaatttac caaaaataat agattgtgta 8821 ggttccgatg gcaaggagag gagacagctt gttaagggcc gtgatgacct gagacaagat 8881 gctgtcatgc aacaggtctt ccagatgtgt aatacattac tgcagagaaa cacggaaact 8941 aggaagagga aattaactat ctgtacttat aaggtggttc ccctctctca gcgaagtggt 9001 gttcttgaat ggtgcacagg aactgtcccc attggtgaat ttcttgttaa caatgaagat 9061 ggtgctcata aaagatacag gccaaatgat ttcagtgcct ttcagtgcca aaagaaaatg 9121 atggaggtgc aaaaaaagtc ttttgaagag aaatatgaag tcttcatgga tgtttgccaa 9181 aattttcaac cagttttccg ttacttctgc atggaaaaat tcttggatcc agctatttgg 9241 tttgagaagc gattggctta tacgcgcagt gtagctactt cttctattgt tggttacata 9301 cttggacttg gtgatagaca tgtacagaat atcttgataa atgagcagtc agcagaactt 9361 gtacatatag atctaggtgt tgcttttgaa cagggcaaaa tccttcctac tcctgagaca 9421 gttcctttta gactcaccag agatattgtg gatggcatgg gcattacggg tgttgaaggt 9481 gtcttcagaa gatgctgtga gaaaaccatg gaagtgatga gaaactctca ggaaactctg 9541 ttaaccattg tagaggtcct tctatatgat ccactctttg actggaccat gaatcctttg 9601 aaagctttgt atttacagca gaggccggaa gatgaaactg agcttcaccc tactctgaat 9661 gcagatgacc aagaatgcaa acgaaatctc agtgatattg accagagttt caacaaagta 9721 gctgaacgtg tcttaatgag actacaagag aaactgaaag gagtggaaga aggcactgtg 9781 ctcagtgttg gtggacaagt gaatttgctc atacagcagg ccatagaccc caaaaatctc 9841 agccgacttt tcccaggatg gaaagcttgg gtgCTCGAGC ACCATCACCA TCACCATCAC 9901 CATTAGGGCC CGATATCTCG ACAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC 9961 TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT 10021 GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT 10081 GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT 10141 GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG 10201 GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG 10261 CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAGCT 10321 GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT 10381 CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC 10441 TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC 10501 CGCCTCCCCG CCTGGAAACG GGGGAGGCTA ACTGAAACAC GGAAGGAGAC AATACCGGAA 10561 GGAACCCGCG CTATGACGGC AATAAAAAGA CAGAATAAAA CGCACGGGTG TTGGGTCGTT 10621 TGTTCATAAA CGCGGGGTTC GGTCCCAGGG CTGGCACTCT GTCGATACCC CACCGAGACC 10681 CCATTGGGGC CAATACGCCC GCGTTTCTTC CTTTTCCCCA CCCCACCCCC CAAGTTCGGG 10741 TGAAGGCCCA GGGCTCGCAG CCAACGTCGG GGCGGCAGGC CCTGCCATAG CAGATCTGCG 10801 CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG 10861 TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT 10921 CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG 10981 GGGCATCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA 11041 TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC 11101 GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC 11161 TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGGGG ATTTCGGCCT ATTGGTTAAA 11221 AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA 11281 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 11341 TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 11401 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 11461 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA 11521 GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA 11581 GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA 11641 GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG 11701 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT 11761 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC 11821 CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG 11881 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG 11941 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA 12001 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA 12061 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT 12121 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC 12181 AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC 12241 TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG 12301 GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT 12361 GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG 12421 CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGCGA 12481 AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT 12541 CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG 12601 CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG 12661 TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC 12721 TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC 12781 TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT 12841 CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG 12901 AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT 12961 GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG 13021 GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC 13081 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 13141 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 13201 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 13261 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 13321 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 13381 GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 13441 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 13501 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 13561 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 13621 GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 13681 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 13741 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 13801 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 13861 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 13921 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT 13981 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC 14041 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT 14101 ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG 14161 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG 14221 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC 14281 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA 14341 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG 14401 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC 14461 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA 14521 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC 14581 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG 14641 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC 14701 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC 14761 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT 14821 ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG 14881 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC 14941 CCGAAAAGTG CCACCTGACG TCGACGGATC GGGAGATCTC CCGATCCCCT ATGGTCGACT 15001 CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATCTGCTCCC TGCTTGTGTG 15061 TTGGAGGTCG CTGAGTAGTG CGCGAGCAAA ATTTAAGCTA CAACAAGGCA AGGCTTGACC 15121 GACAATTGCA TGAAGAATCT GCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.