pCMV-RAD50-3×Myc-Neo plasmid

pCMV-RAD50-3×Myc-Neo plasmid

PVT88863

pCMV-RAD50-3×Myc-Neo plasmid Information

Plasmid name: pCMV-RAD50(human)-3×Myc-Neo
Alias: NM_005732.4;hRad50; NBSLD; RAD502
Gene lenght: 3936bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-RAD50-3×Myc-Neo plasmid Description

pCMV-RAD50-3×Myc-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-RAD50-3×Myc-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatgtc ccggatcgaa 721 aagatgagca ttctgggcgt gcggagtttt ggaatagagg acaaagataa gcaaattatc 781 actttcttca gcccccttac aattttggtt ggacccaatg gggcgggaaa gacgaccatc 841 attgaatgtc taaaatatat ttgtactgga gatttccctc ctggaaccaa aggaaataca 901 tttgtacacg atcccaaggt tgctcaagaa acagatgtga gagcccagat tcgtctgcaa 961 tttcgtgatg tcaatggaga acttatagct gtgcaaagat ctatggtgtg tactcagaaa 1021 agcaaaaaga cagaatttaa aactctggaa ggagtcatta ctagaacaaa gcatggtgaa 1081 aaggtcagtc tgagctctaa gtgtgcagaa attgaccgag aaatgatcag ttctcttggg 1141 gtttccaagg ctgtgctaaa taatgtcatt ttctgtcatc aagaagattc taattggcct 1201 ttaagtgaag gaaaggcttt gaagcaaaag tttgatgaga ttttttcagc aacaagatac 1261 attaaagcct tagaaacact tcggcaggta cgtcagacac aaggtcagaa agtaaaagaa 1321 tatcaaatgg aactaaaata tctgaagcaa tataaggaaa aagcttgtga gattcgtgat 1381 cagattacaa gtaaggaagc ccagttaaca tcttcaaagg aaattgtcaa atcctatgag 1441 aatgaacttg atccattgaa gaatcgtcta aaagaaattg aacataatct ctctaaaata 1501 atgaaacttg acaatgaaat taaagccttg gatagccgaa agaagcaaat ggagaaagat 1561 aatagtgaac tggaagagaa aatggaaaag gtttttcaag ggactgatga gcaactaaat 1621 gacttatatc acaatcacca gagaacagta agggagaaag aaaggaaatt ggtagactgt 1681 catcgtgaac tggaaaaact aaataaagaa tctaggcttc tcaatcagga aaaatcagaa 1741 ctgcttgttg aacagggtcg tctacagctg caagcagatc gccatcaaga acatatccga 1801 gctagagatt cattaattca gtctttggca acacagctag aattggatgg ctttgagcgt 1861 ggaccattca gtgaaagaca gattaaaaat tttcacaaac ttgtgagaga gagacaagaa 1921 ggggaagcaa aaactgccaa ccaactgatg aatgactttg cagaaaaaga gactctgaaa 1981 caaaaacaga tagatgagat aagagataag aaaactggac tgggaagaat aattgagtta 2041 aaatcagaaa tcctaagtaa gaagcagaat gagctgaaaa atgtgaagta tgaattacag 2101 cagttggaag gatcttcaga caggattctt gaactggacc aggagctcat aaaagctgaa 2161 cgtgagttaa gcaaggctga gaaaaacagc aatgtagaaa ccttaaaaat ggaagtaata 2221 agtctccaaa atgaaaaagc agacttagac aggaccctgc gtaaacttga ccaggagatg 2281 gagcagttaa accatcatac aacaacacgt acccaaatgg agatgctgac caaagacaaa 2341 gctgacaaag atgaacaaat cagaaaaata aaatctaggc acagtgatga attaacctca 2401 ctgttgggat attttcccaa caaaaaacag cttgaagact ggctacatag taaatcaaaa 2461 gaaattaatc agaccaggga cagacttgcc aaattgaaca aggaactagc ttcatctgag 2521 cagaataaaa atcatataaa taatgaacta aaaagaaagg aagagcagtt gtccagttac 2581 gaagacaagc tgtttgatgt ttgtggtagc caggattttg aaagtgattt agacaggctt 2641 aaagaggaaa ttgaaaaatc atcaaaacag cgagccatgc tggctggagc cacagcagtt 2701 tactcccagt tcattactca gctaacagac gaaaaccagt catgttgccc cgtttgtcag 2761 agagtttttc agacagaggc tgagttacaa gaagtcatca gtgatttgca gtctaaactg 2821 cgacttgctc cagataaact caagtcaaca gaatcagagc taaaaaaaaa ggaaaagcgg 2881 cgtgatgaaa tgctgggact tgtgcccatg aggcaaagca taattgattt gaaggagaag 2941 gaaataccag aattaagaaa caaactgcag aatgtcaata gagacataca gcgcctaaag 3001 aacgacatag aagaacaaga aacactcttg ggtacaataa tgcctgaaga agaaagtgcc 3061 aaagtatgcc tgacagatgt tacaattatg gagaggttcc agatggaact taaagatgtt 3121 gaaagaaaaa ttgcacaaca agcagctaag ctacaaggaa tagacttaga tcgaactgtc 3181 caacaagtca accaggagaa acaagagaaa cagcacaagt tagacacagt ttctagtaag 3241 attgaattga atcgtaagct tatacaggac cagcaggaac agattcaaca tctaaaaagt 3301 acaacaaatg agctaaaatc tgagaaactt cagatatcca ctaatttgca acgtcgtcag 3361 caactggagg agcagactgt ggaattatcc actgaagttc agtctttgta cagagagata 3421 aaggatgcta aagagcaggt aagccctttg gaaacaacat tggaaaagtt ccagcaagaa 3481 aaagaagaat taatcaacaa aaaaaataca agcaacaaaa tagcacagga taaactgaat 3541 gatattaaag agaaggttaa aaatattcat ggctatatga aagacattga gaattatatt 3601 caagatggga aagacgacta taagaagcaa aaagaaactg aacttaataa agtaatagct 3661 caactaagtg aatgcgagaa acacaaagaa aagataaatg aagatatgag actcatgaga 3721 caagatattg atacacagaa gatacaagaa aggtggctac aagataacct tactttaaga 3781 aaaagaaatg aggaactaaa agaagttgaa gaagaaagaa aacaacattt gaaggaaatg 3841 ggtcaaatgc aggttttgca aatgaaaagt gaacatcaga agttggaaga gaacatagac 3901 aatataaaaa gaaatcataa tttggcatta gggcgacaga aaggttatga agaagaaatt 3961 attcatttta agaaagaact tcgagaacca caatttcggg atgctgagga aaagtataga 4021 gaaatgatga ttgttatgag gacaacagaa cttgtgaaca aggatctgga tatttattat 4081 aagactcttg accaagcaat aatgaaattt cacagtatga aaatggaaga aatcaataaa 4141 attatacgtg acctgtggcg aagtacctat cgtggacaag atattgaata catagaaata 4201 cggtctgatg ccgatgaaaa tgtatcagct tctgataaaa ggcggaatta taactaccga 4261 gtggtgatgc tgaagggaga cacagccttg gatatgcgag gacgatgcag tgctggacaa 4321 aaggtattag cctcactcat cattcgcctg gccctggctg aaacgttctg cctcaactgt 4381 ggcatcattg ccttggatga gccaacaaca aatcttgacc gagaaaacat tgaatctctt 4441 gcacatgctc tggttgagat aataaaaagt cgctcacagc agcgtaactt ccagcttctg 4501 gtaatcactc atgatgaaga ttttgtggag cttttaggac gttctgaata tgtggagaaa 4561 ttctacagga ttaaaaagaa catcgatcag tgctcagaga ttgtgaaatg cagtgttagc 4621 tccctgggat tcaatgttca tCTCGAGGAA CAAAAACTAA TATCAGAGGA AGATTTGGAA 4681 CAGAAACTAA TCAGTGAAGA AGATTTAGAG CAAAAGTTAA TTTCCGAGGA GGACTTATAG 4741 TGAGGGCCCG ATATCTCGAC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG 4801 GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT 4861 ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC 4921 TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT 4981 TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA 5041 CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT 5101 GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAGCTGA 5161 CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT 5221 GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC 5281 TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG 5341 CCTCCCCGCC TGGAAACGGG GGAGGCTAAC TGAAACACGG AAGGAGACAA TACCGGAAGG 5401 AACCCGCGCT ATGACGGCAA TAAAAAGACA GAATAAAACG CACGGGTGTT GGGTCGTTTG 5461 TTCATAAACG CGGGGTTCGG TCCCAGGGCT GGCACTCTGT CGATACCCCA CCGAGACCCC 5521 ATTGGGGCCA ATACGCCCGC GTTTCTTCCT TTTCCCCACC CCACCCCCCA AGTTCGGGTG 5581 AAGGCCCAGG GCTCGCAGCC AACGTCGGGG CGGCAGGCCC TGCCATAGCA GATCTGCGCA 5641 GCTGGGGCTC TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG 5701 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 5761 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 5821 GCATCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT 5881 AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 5941 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 6001 TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGGGGAT TTCGGCCTAT TGGTTAAAAA 6061 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTAATTCTG TGGAATGTGT GTCAGTTAGG 6121 GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA 6181 GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT 6241 GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC 6301 TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA 6361 GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG 6421 CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA 6481 GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC 6541 CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA 6601 TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT 6661 GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC 6721 GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT 6781 ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT 6841 ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT 6901 CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT 6961 CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG 7021 GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT 7081 GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG 7141 TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG 7201 CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG 7261 CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGCGAAA 7321 TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT 7381 ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG 7441 GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT 7501 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA 7561 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA 7621 GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA 7681 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG 7741 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT 7801 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC 7861 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG 7921 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 7981 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 8041 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 8101 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 8161 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 8221 GAAGCGTGGC GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 8281 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 8341 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 8401 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 8461 GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 8521 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 8581 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 8641 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 8701 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 8761 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA 8821 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG 8881 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 8941 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG 9001 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 9061 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 9121 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC 9181 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC 9241 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC 9301 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 9361 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 9421 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT 9481 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 9541 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA 9601 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 9661 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 9721 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 9781 GAAAAGTGCC ACCTGACGTC GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT 9841 CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT 9901 GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA 9961 CAATTGCATG AAGAATCTGC TTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.