pLV3-CMV-ZNF503-3×FLAG-EF1a-Co

pLV3-CMV-ZNF503-3×FLAG-EF1a-CopGFP-Neo plasmid

PVT94699

pLV3-CMV-ZNF503-3×FLAG-EF1a-CopGFP-Neo plasmid Information

Plasmid name: pLV3-CMV-ZNF503(human)-3×FLAG-EF1a-CopGFP-Neo
Alias: NM_032772.6;Nlz2; NOLZ-1; NOLZ1
Gene lenght: 1938bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ZNF503-3×FLAG-EF1a-CopGFP-Neo plasmid Description

pLV3-CMV-ZNF503-3×FLAG-EF1a-CopGFP-Neo is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-ZNF503-3×FLAG-EF1a-CopGFP-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatgagcac agcgccctcg ctttctgccc taagaagcag 2341 taagcacagc ggcggcggcg gcggcggagg cggaggcggc ggtgcagacc ctgcctggac 2401 cagcgcgctc tctggaaata gctccggccc cggcccaggc tcgtccccgg ccggcagcac 2461 caagcctttt gtgcacgccg tgcccccctc tgaccccctg cgccaggcca accgcctgcc 2521 aatcaaggtg ctgaagatgc tgacggcacg aactggccac attttgcacc ccgagtacct 2581 gcagcccctg ccttccacgc cggtcagccc catcgagctc gatgccaaga agagcccgct 2641 ggcgctgttg gcgcaaacat gttcgcagat cgggaagccc gacccctcgc cctcctccaa 2701 actctcctcg gttgcctcca acgggggcgg cgcgggcggt gccggcggcg gtgctgcggg 2761 cgacaaggac accaaatcgg gccccctgaa gctgagcgac atcggcgtgg aggacaagtc 2821 gagtttcaag ccgtactcca aacccggctc ggataagaag gagccgggag gcggcggtgg 2881 aggcggtggc ggtggcgggg gcggcggcgg gggtgtttcg tcggagaagt cgggattccg 2941 ggtaccgagc gccacctgcc agccattcac gcccaggaca ggcagcccga gctccagcgc 3001 ctcggcctgc tcgccgggag gtatgctgtc ctcggccggg ggtgccccgg agggcaagga 3061 cgacaagaaa gacaccgacg tgggcggcgg tggcaagggc accgggggcg cctcggccga 3121 agggggaccc acggggctgg cacacggccg gattagctgc ggcggcggga ttaatgtgga 3181 tgtgaaccag catccggatg ggggcccggg aggcaaggct ctgggctcgg actgcggcgg 3241 ttcatcgggc tccagctccg gctccggccc cagcgcgccc acctcctcct cagtgttggg 3301 ctctgggctg gtggctcccg tgtcacccta caagccgggc cagacagtgt tccctctgcc 3361 tcccgcgggt atgacctacc caggcagcct ggccggggcc tacgccggct acccgcccca 3421 gttcctgcca cacggcgtgg cacttgaccc caccaagccg ggcagcctgg tgggggcgca 3481 gctggcggcg gccgcggccg ggtctctggg ctgcagtaag ccggccggct ccagcccttt 3541 ggccggagcg tctccgccgt ccgtgatgac agccagtttg tgccgggacc cttactgcct 3601 cagctaccac tgcgctagcc acctggcagg ggcggcggcc gccagcgctt cttgcgcaca 3661 tgatccggct gctgcggctg cggcgctgaa gtccggatac ccgctggtgt accccacgca 3721 cccgctgcac ggtgtgcact cctcgctaac ggccgccgcg gctgctggcg ccacaccgcc 3781 ctccctggcc ggccaccccc tctaccccta cggctttatg ctccctaacg acccactccc 3841 ccacatctgc aactgggtgt cggccaacgg gccgtgcgac aagcgcttcg ccacgtccga 3901 agagctgctg agccacttgc ggacccatac ggcatttccc gggacagaca aactgctgtc 3961 gggctacccc agctcgtcgt ctctggccag cgctgccgcg gccgccatgg cttgccacat 4021 gcacatcccc acctcgggcg caccgggcag ccctgggacg ctggcgctgc gcagccccca 4081 ccacgcgctg ggactcagca gccgctacca cccctactcc aagagcccgc ttcccacgcc 4141 tggcgccccc gtgccggtgc ccgccgccac cggaccgtac tactccccct acgccctcta 4201 cggacagaga ctgaccaccg cctcggcgct ggggtatcag ggatccGACT ACAAAGACCA 4261 TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT GACGATGACA AGTAgcggcc 4321 gcGaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt 4381 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg 4441 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga 4501 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag 4561 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg 4621 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg 4681 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc 4741 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc 4801 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc 4861 ggcgcctacg ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat 4921 cgagtgccgc atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga 4981 gggcaccccc aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac 5041 cttcagcccc tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta 5101 ccccagcggc tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac 5161 ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga 5221 ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt 5281 gatcttcacc gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg 5341 cgataacgtg ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta 5401 cagcttcgtg gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca 5461 gaacgggggc cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct 5521 gggcatcgtg gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc 5581 tcagtcgtcc aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga 5641 gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc ctGCCACCat 5701 ggctgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 5761 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 5821 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 5881 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 5941 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 6001 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 6061 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 6121 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 6181 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg 6241 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 6301 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 6361 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 6421 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 6481 cgagttcttc tgaGTCGACA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG 6541 TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA 6601 TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT 6661 GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT 6721 TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC 6781 TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG 6841 CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC 6901 GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG 6961 CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT 7021 GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC 7081 CTCCCCGCgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 7141 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaGata agatctgctt 7201 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 7261 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 7321 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 7381 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 7441 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa 7501 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 7561 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc 7621 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 7681 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 7741 tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa 7801 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 7861 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 7921 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 7981 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 8041 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 8101 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 8161 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 8221 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 8281 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 8341 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 8401 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 8461 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 8521 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 8581 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 8641 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 8701 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 8761 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 8821 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 8881 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 8941 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 9001 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 9061 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 9121 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 9181 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 9241 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 9301 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 9361 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 9421 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 9481 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 9541 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 9601 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 9661 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 9721 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 9781 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 9841 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 9901 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 9961 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg 10021 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 10081 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc 10141 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac 10201 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca 10261 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg 10321 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta 10381 aaacgacggc cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.