pTac-GST-Uchl5 plasmid

pTac-GST-Uchl5 plasmid

PVT91200

pTac-GST-Uchl5 plasmid Information

Plasmid name: pTac-GST-Uchl5(mouse)
Alias: NM_019562.2;5830413B11Rik; Uch37
Gene lenght: 990bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: T7
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pGEX-5'
Reverse primer:
Copy number: High
Induction: IPTG Induction

pTac-GST-Uchl5 plasmid Description

pTac-GST-Uchl5 is a plasmid suitable for protein expression in Escherichia coli.

pTac-GST-Uchl5 plasmid Sequence

1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gttccgcgtg gatccatgtc gagcaatgcc ggggagtggt 961 gtctcatgga aagcgacccc ggggtcttca ccgagctcat taaaggattc ggttgccgag 1021 gggcccaagt agaagaaata tggagcttag aacctgagag ttttgaaaag ctaaagccag 1081 ttcatgggtt aatttttctt ttcaagtggc agccaggaga agaacctgct ggctctgtgg 1141 tccaggactc cagacttgaa acaatatttt ttgccaagca ggtaattaat aatgcttgtg 1201 ccactcaggc tatcgtaagt gtactactga actgtacgca tcaagatgtg catttaggag 1261 agacattatc agaatttaaa gaattttctc aaagttttga tgcagctatg aaggggctgg 1321 cattgagtaa ctcagatgtg attcgacaag tgcacaacag ttttgccagg cagcagatgt 1381 ttgaatttga tacaaagaca ccagccaaag aggaggatgc ttttcacttt gtcagttacg 1441 ttccagtcaa tgggaggctg tatgaactag acgggttaag agagggccca attgatttag 1501 gtgcatgcaa tcaagatgac tggattactg cagtgaggcc agtaatagag aaaaggattc 1561 aaaagtatag tgaaggagaa attcgattta atttaatggc cattgtatct gacagaaaaa 1621 tgatatatga acagaagata gcagagttac aaagacaact tgcagaggag gaacccatgg 1681 atacagatca aggtagtacc gtgttaagtg ctattcagtc agaagttgcc agaaaccaga 1741 tgcttattga agaagaggta caaaagttaa agagatataa gattgaaaat atcagaagga 1801 agcataatta cttgcctttc attatggaat tgttaaagac cttagctgag caccagcagt 1861 taattccact tgtggaaaag gcaaaagaaa aacagaatgc aaagaaagct caggaaacta 1921 agtgactcga gcggccgcat cgtgactgac tgacgatctg cctcgcgcgt ttcggtgatg 1981 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg 2041 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg 2101 cagccatgac ccagtcacgt agcgatagcg gagtgtataa ttcttgaaga cgaaagggcc 2161 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag 2221 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 2281 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 2341 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 2401 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 2461 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 2521 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 2581 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 2641 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 2701 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 2761 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 2821 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 2881 ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 2941 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 3001 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 3061 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 3121 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 3181 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 3241 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 3301 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 3361 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 3421 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 3481 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 3541 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 3601 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 3661 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 3721 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 3781 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 3841 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 3901 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 3961 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 4021 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 4081 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 4141 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc 4201 gcataaattc cgacaccatc gaatggtgca aaacctttcg cggtatggca tgatagcgcc 4261 cggaagagag tcaattcagg gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag 4321 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt 4381 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc 4441 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc 4501 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg 4561 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg 4621 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc 4681 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct 4741 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg 4801 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt 4861 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc 4921 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc 4981 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc 5041 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag 5101 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac 5161 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc 5221 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg 5281 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 5341 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc 5401 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt 5461 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgg attcactggc 5521 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc 5581 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc 5641 ccaacagttg cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc 5701 ggtgccggaa agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc 5761 aaactggcag atgcacggtt acgatgcgcc catctacacc aacgtaacct atcccattac 5821 ggtcaatccg ccgtttgttc ccacggagaa tccgacgggt tgttactcgc tcacatttaa 5881 tgttgatgaa agctggctac aggaaggcca gacgcgaatt atttttgatg gcgttggaat 5941 t

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.