pCAMBIA1300-35S-3×FLAG-Hyg pla

pCAMBIA1300-35S-3×FLAG-Hyg plasmid

PVT92887

pCAMBIA1300-35S-3×FLAG-Hyg plasmid Information

Plasmid name: pCAMBIA1300-35S-3×FLAG-Hyg
Alias: -
Gene lenght:
Host: Plant
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Kan
Screening marker: Hyg
Fluorescent labeling:
Promoter: 35S
Replicon: pVS1
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: M13R
Copy number: High
Induction:

pCAMBIA1300-35S-3×FLAG-Hyg plasmid Description

pCAMBIA1300-35S-3×FLAG-Hyg This is a plasmid suitable for protein expression in plants.

pCAMBIA1300-35S-3×FLAG-Hyg plasmid Sequence

1 AATTCTGAGA CTTTTCAACA AAGGGTAATA TCCGGAAACC TCCTCGGATT CCATTGCCCA 61 GCTATCTGTC ACTTTATTGT GAAGATAGTG GAAAAGGAAG GTGGCTCCTA CAAATGCCAT 121 CATTGCGATA AAGGAAAGGC CATCGTTGAA GATGCCTCTG CCGACAGTGG TCCCAAAGAT 181 GGACCCCCAC CCACGAGGAG CATCGTGGAA AAAGAAGACG TTCCAACCAC GTCTTCAAAG 241 CAAGTGGATT GATGTGATAT CTCCACTGAC GTAAGGGATG ACGCACAATC CCACTATCCT 301 TCGCAAGACC CTTCCTCTAT ATAAGGAAGT TCATTTCATT TGGAGAGAAC Acgggggacg 361 agctcggtac ccggggatcc GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA 421 TCGACTACAA GGATGACGAT GACAAGTAGg tcgacctgca ggcatgcGAT CGTTCAAACA 481 TTTGGCAATA AAGTTTCTTA AGATTGAATC CTGTTGCCGG TCTTGCGATG ATTATCATAT 541 AATTTCTGTT GAATTACGTT AAGCATGTAA TAATTAACAT GTAATGCATG ACGTTATTTA 601 TGAGATGGGT TTTTATGATT AGAGTCCCGC AATTATACAT TTAATACGCG ATAGAAAACA 661 AAATATAGCG CGCAAACTAG GATAAATTAT CGCGCGCGGT GTCATCTATG TTACTAGATC 721 AAGCTTGGCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA 781 ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG 841 CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGCTAGA GCAGCTTGAG 901 CTTGGATCAG ATTGTCGTTT CCCGCCTTCA GTTTAAACTA TCAGTGTTTG ACAGGATATA 961 TTGGCGGGTA AACCTAAGAG AAAAGAGCGT TTATTAGAAT AACGGATATT TAAAAGGGCG 1021 TGAAAAGGTT TATCCGTTCG TCCATTTGTA TGTGCATGCC AACCACAGGG TTCCCCTCGG 1081 GATCAAAGTA CTTTGATCCA ACCCCTCCGC TGCTATAGTG CAGTCGGCTT CTGACGTTCA 1141 GTGCAGCCGT CTTCTGAAAA CGACATGTCG CACAAGTCCT AAGTTACGCG ACAGGCTGCC 1201 GCCCTGCCCT TTTCCTGGCG TTTTCTTGTC GCGTGTTTTA GTCGCATAAA GTAGAATACT 1261 TGCGACTAGA ACCGGAGACA TTACGCCATG AACAAGAGCG CCGCCGCTGG CCTGCTGGGC 1321 TATGCCCGCG TCAGCACCGA CGACCAGGAC TTGACCAACC AACGGGCCGA ACTGCACGCG 1381 GCCGGCTGCA CCAAGCTGTT TTCCGAGAAG ATCACCGGCA CCAGGCGCGA CCGCCCGGAG 1441 CTGGCCAGGA TGCTTGACCA CCTACGCCCT GGCGACGTTG TGACAGTGAC CAGGCTAGAC 1501 CGCCTGGCCC GCAGCACCCG CGACCTACTG GACATTGCCG AGCGCATCCA GGAGGCCGGC 1561 GCGGGCCTGC GTAGCCTGGC AGAGCCGTGG GCCGACACCA CCACGCCGGC CGGCCGCATG 1621 GTGTTGACCG TGTTCGCCGG CATTGCCGAG TTCGAGCGTT CCCTAATCAT CGACCGCACC 1681 CGGAGCGGGC GCGAGGCCGC CAAGGCCCGA GGCGTGAAGT TTGGCCCCCG CCCTACCCTC 1741 ACCCCGGCAC AGATCGCGCA CGCCCGCGAG CTGATCGACC AGGAAGGCCG CACCGTGAAA 1801 GAGGCGGCTG CACTGCTTGG CGTGCATCGC TCGACCCTGT ACCGCGCACT TGAGCGCAGC 1861 GAGGAAGTGA CGCCCACCGA GGCCAGGCGG CGCGGTGCCT TCCGTGAGGA CGCATTGACC 1921 GAGGCCGACG CCCTGGCGGC CGCCGAGAAT GAACGCCAAG AGGAACAAGC ATGAAACCGC 1981 ACCAGGACGG CCAGGACGAA CCGTTTTTCA TTACCGAAGA GATCGAGGCG GAGATGATCG 2041 CGGCCGGGTA CGTGTTCGAG CCGCCCGCGC ACGTCTCAAC CGTGCGGCTG CATGAAATCC 2101 TGGCCGGTTT GTCTGATGCC AAGCTGGCGG CCTGGCCGGC CAGCTTGGCC GCTGAAGAAA 2161 CCGAGCGCCG CCGTCTAAAA AGGTGATGTG TATTTGAGTA AAACAGCTTG CGTCATGCGG 2221 TCGCTGCGTA TATGATGCGA TGAGTAAATA AACAAATACG CAAGGGGAAC GCATGAAGGT 2281 TATCGCTGTA CTTAACCAGA AAGGCGGGTC AGGCAAGACG ACCATCGCAA CCCATCTAGC 2341 CCGCGCCCTG CAACTCGCCG GGGCCGATGT TCTGTTAGTC GATTCCGATC CCCAGGGCAG 2401 TGCCCGCGAT TGGGCGGCCG TGCGGGAAGA TCAACCGCTA ACCGTTGTCG GCATCGACCG 2461 CCCGACGATT GACCGCGACG TGAAGGCCAT CGGCCGGCGC GACTTCGTAG TGATCGACGG 2521 AGCGCCCCAG GCGGCGGACT TGGCTGTGTC CGCGATCAAG GCAGCCGACT TCGTGCTGAT 2581 TCCGGTGCAG CCAAGCCCTT ACGACATATG GGCCACCGCC GACCTGGTGG AGCTGGTTAA 2641 GCAGCGCATT GAGGTCACGG ATGGAAGGCT ACAAGCGGCC TTTGTCGTGT CGCGGGCGAT 2701 CAAAGGCACG CGCATCGGCG GTGAGGTTGC CGAGGCGCTG GCCGGGTACG AGCTGCCCAT 2761 TCTTGAGTCC CGTATCACGC AGCGCGTGAG CTACCCAGGC ACTGCCGCCG CCGGCACAAC 2821 CGTTCTTGAA TCAGAACCCG AGGGCGACGC TGCCCGCGAG GTCCAGGCGC TGGCCGCTGA 2881 AATTAAATCA AAACTCATTT GAGTTAATGA GGTAAAGAGA AAATGAGCAA AAGCACAAAC 2941 ACGCTAAGTG CCGGCCGTCC GAGCGCACGC AGCAGCAAGG CTGCAACGTT GGCCAGCCTG 3001 GCAGACACGC CAGCCATGAA GCGGGTCAAC TTTCAGTTGC CGGCGGAGGA TCACACCAAG 3061 CTGAAGATGT ACGCGGTACG CCAAGGCAAG ACCATTACCG AGCTGCTATC TGAATACATC 3121 GCGCAGCTAC CAGAGTAAAT GAGCAAATGA ATAAATGAGT AGATGAATTT TAGCGGCTAA 3181 AGGAGGCGGC ATGGAAAATC AAGAACAACC AGGCACCGAC GCCGTGGAAT GCCCCATGTG 3241 TGGAGGAACG GGCGGTTGGC CAGGCGTAAG CGGCTGGGTT GTCTGCCGGC CCTGCAATGG 3301 CACTGGAACC CCCAAGCCCG AGGAATCGGC GTGAGCGGTC GCAAACCATC CGGCCCGGTA 3361 CAAATCGGCG CGGCGCTGGG TGATGACCTG GTGGAGAAGT TGAAGGCCGC GCAGGCCGCC 3421 CAGCGGCAAC GCATCGAGGC AGAAGCACGC CCCGGTGAAT CGTGGCAAGC GGCCGCTGAT 3481 CGAATCCGCA AAGAATCCCG GCAACCGCCG GCAGCCGGTG CGCCGTCGAT TAGGAAGCCG 3541 CCCAAGGGCG ACGAGCAACC AGATTTTTTC GTTCCGATGC TCTATGACGT GGGCACCCGC 3601 GATAGTCGCA GCATCATGGA CGTGGCCGTT TTCCGTCTGT CGAAGCGTGA CCGACGAGCT 3661 GGCGAGGTGA TCCGCTACGA GCTTCCAGAC GGGCACGTAG AGGTTTCCGC AGGGCCGGCC 3721 GGCATGGCCA GTGTGTGGGA TTACGACCTG GTACTGATGG CGGTTTCCCA TCTAACCGAA 3781 TCCATGAACC GATACCGGGA AGGGAAGGGA GACAAGCCCG GCCGCGTGTT CCGTCCACAC 3841 GTTGCGGACG TACTCAAGTT CTGCCGGCGA GCCGATGGCG GAAAGCAGAA AGACGACCTG 3901 GTAGAAACCT GCATTCGGTT AAACACCACG CACGTTGCCA TGCAGCGTAC GAAGAAGGCC 3961 AAGAACGGCC GCCTGGTGAC GGTATCCGAG GGTGAAGCCT TGATTAGCCG CTACAAGATC 4021 GTAAAGAGCG AAACCGGGCG GCCGGAGTAC ATCGAGATCG AGCTAGCTGA TTGGATGTAC 4081 CGCGAGATCA CAGAAGGCAA GAACCCGGAC GTGCTGACGG TTCACCCCGA TTACTTTTTG 4141 ATCGATCCCG GCATCGGCCG TTTTCTCTAC CGCCTGGCAC GCCGCGCCGC AGGCAAGGCA 4201 GAAGCCAGAT GGTTGTTCAA GACGATCTAC GAACGCAGTG GCAGCGCCGG AGAGTTCAAG 4261 AAGTTCTGTT TCACCGTGCG CAAGCTGATC GGGTCAAATG ACCTGCCGGA GTACGATTTG 4321 AAGGAGGAGG CGGGGCAGGC TGGCCCGATC CTAGTCATGC GCTACCGCAA CCTGATCGAG 4381 GGCGAAGCAT CCGCCGGTTC CTAATGTACG GAGCAGATGC TAGGGCAAAT TGCCCTAGCA 4441 GGGGAAAAAG GTCGAAAAGG TCTCTTTCCT GTGGATAGCA CGTACATTGG GAACCCAAAG 4501 CCGTACATTG GGAACCGGAA CCCGTACATT GGGAACCCAA AGCCGTACAT TGGGAACCGG 4561 TCACACATGT AAGTGACTGA TATAAAAGAG AAAAAAGGCG ATTTTTCCGC CTAAAACTCT 4621 TTAAAACTTA TTAAAACTCT TAAAACCCGC CTGGCCTGTG CATAACTGTC TGGCCAGCGC 4681 ACAGCCGAAG AGCTGCAAAA AGCGCCTACC CTTCGGTCGC TGCGCTCCCT ACGCCCCGCC 4741 GCTTCGCGTC GGCCTATCGC GGCCGCTGGC CGCTCAAAAA TGGCTGGCCT ACGGCCAGGC 4801 AATCTACCAG GGCGCGGACA AGCCGCGCCG TCGCCACTCG ACCGCCGGCG CCCACATCAA 4861 GGCACCCTGC CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC 4921 GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC 4981 GTCAGCGGGT GTTGGCGGGT GTCGGGGCGC AGCCATGACC CAGTCACGTA GCGATAGCGG 5041 AGTGTATACT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATG 5101 CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG CTCTTCCGCT 5161 TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC 5221 TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA 5281 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT 5341 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC 5401 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT 5461 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG 5521 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG 5581 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT 5641 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG 5701 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC 5761 GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA 5821 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT 5881 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT 5941 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGCAT 6001 TCTAGGTACT AAAACAATTC ATCCAGTAAA ATATAATATT TTATTTTCTC CCAATCAGGC 6061 TTGATCCCCA GTAAGTCAAA AAATAGCTCG ACATACTGTT CTTCCCCGAT ATCCTCCCTG 6121 ATCGACCGGA CGCAGAAGGC AATGTCATAC CACTTGTCCG CCCTGCCGCT TCTCCCAAGA 6181 TCAATAAAGC CACTTACTTT GCCATCTTTC ACAAAGATGT TGCTGTCTCC CAGGTCGCCG 6241 TGGGAAAAGA CAAGTTCCTC TTCGGGCTTT TCCGTCTTTA AAAAATCATA CAGCTCGCGC 6301 GGATCTTTAA ATGGAGTGTC TTCTTCCCAG TTTTCGCAAT CCACATCGGC CAGATCGTTA 6361 TTCAGTAAGT AATCCAATTC GGCTAAGCGG CTGTCTAAGC TATTCGTATA GGGACAATCC 6421 GATATGTCGA TGGAGTGAAA GAGCCTGATG CACTCCGCAT ACAGCTCGAT AATCTTTTCA 6481 GGGCTTTGTT CATCTTCATA CTCTTCCGAG CAAAGGACGC CATCGGCCTC ACTCATGAGC 6541 AGATTGCTCC AGCCATCATG CCGTTCAAAG TGCAGGACCT TTGGAACAGG CAGCTTTCCT 6601 TCCAGCCATA GCATCATGTC CTTTTCCCGT TCCACATCAT AGGTGGTCCC TTTATACCGG 6661 CTGTCCGTCA TTTTTAAATA TAGGTTTTCA TTTTCTCCCA CCAGCTTATA TACCTTAGCA 6721 GGAGACATTC CTTCCGTATC TTTTACGCAG CGGTATTTTT CGATCAGTTT TTTCAATTCC 6781 GGTGATATTC TCATTTTAGC CATTTATTAT TTCCTTCCTC TTTTCTACAG TATTTAAAGA 6841 TACCCCAAGA AGCTAATTAT AACAAGACGA ACTCCAATTC ACTGTTCCTT GCATTCTAAA 6901 ACCTTAAATA CCAGAAAACA GCTTTTTCAA AGTTGTTTTC AAAGTTGGCG TATAACATAG 6961 TATCGACGGA GCCGATTTTG AAACCGCGGT GATCACAGGC AGCAACGCTC TGTCATCGTT 7021 ACAATCAACA TGCTACCCTC CGCGAGATCA TCCGTGTTTC AAACCCGGCA GCTTAGTTGC 7081 CGTTCTTCCG AATAGCATCG GTAACATGAG CAAAGTCTGC CGCCTTACAA CGGCTCTCCC 7141 GCTGACGCCG TCCCGGACTG ATGGGCTGCC TGTATCGAGT GGTGATTTTG TGCCGAGCTG 7201 CCGGTCGGGG AGCTGTTGGC TGGCTGGTGG CAGGATATAT TGTGGTGTAA ACAAATTGAC 7261 GCTTAGACAA CTTAATAACA CATTGCGGAC GTTTTTAATG TACTGAATTA ACGCCGAATT 7321 AATTCGGGGG ATCTGGATTT TAGTACTGGA TTTTGGTTTT AGGAATTAGA AATTTTATTG 7381 ATAGAAGTAT TTTACAAATA CAAATACATA CTAAGGGTTT CTTATATGCT CAACACATGA 7441 GCGAAACCCT ATAGGAACCC TAATTCCCTT ATCTGGGAAC TACTCACACA TTATTATGGA 7501 GAAACTCGAG CTTGTCGATC GACAGATCCG GTCGGCATCT ACTCTATTTC TTTGCCCTCG 7561 GACGAGTGCT GGGGCGTCGG TTTCCACTAT CGGCGAGTAC TTCTACACAG CCATCGGTCC 7621 AGACGGCCGC GCTTCTGCGG GCGATTTGTG TACGCCCGAC AGTCCCGGCT CCGGATCGGA 7681 CGATTGCGTC GCATCGACCC TGCGCCCAAG CTGCATCATC GAAATTGCCG TCAACCAAGC 7741 TCTGATAGAG TTGGTCAAGA CCAATGCGGA GCATATACGC CCGGAGTCGT GGCGATCCTG 7801 CAAGCTCCGG ATGCCTCCGC TCGAAGTAGC GCGTCTGCTG CTCCATACAA GCCAACCACG 7861 GCCTCCAGAA GAAGATGTTG GCGACCTCGT ATTGGGAATC CCCGAACATC GCCTCGCTCC 7921 AGTCAATGAC CGCTGTTATG CGGCCATTGT CCGTCAGGAC ATTGTTGGAG CCGAAATCCG 7981 CGTGCACGAG GTGCCGGACT TCGGGGCAGT CCTCGGCCCA AAGCATCAGC TCATCGAGAG 8041 CCTGCGCGAC GGACGCACTG ACGGTGTCGT CCATCACAGT TTGCCAGTGA TACACATGGG 8101 GATCAGCAAT CGCGCATATG AAATCACGCC ATGTAGTGTA TTGACCGATT CCTTGCGGTC 8161 CGAATGGGCC GAACCCGCTC GTCTGGCTAA GATCGGCCGC AGCGATCGCA TCCATAGCCT 8221 CCGCGACCGG TTGTAGAACA GCGGGCAGTT CGGTTTCAGG CAGGTCTTGC AACGTGACAC 8281 CCTGTGCACG GCGGGAGATG CAATAGGTCA GGCTCTCGCT AAACTCCCCA ATGTCAAGCA 8341 CTTCCGGAAT CGGGAGCGCG GCCGATGCAA AGTGCCGATA AACATAACGA TCTTTGTAGA 8401 AACCATCGGC GCAGCTATTT ACCCGCAGGA CATATCCACG CCCTCCTACA TCGAAGCTGA 8461 AAGCACGAGA TTCTTCGCCC TCCGAGAGCT GCATCAGGTC GGAGACGCTG TCGAACTTTT 8521 CGATCAGAAA CTTCTCGACA GACGTCGCGG TGAGTTCAGG CTTTTTCATA TCTCATTGCC 8581 CCCCGGGATC TGCGAAAGCT CGAGAGAGAT AGATTTGTAG AGAGAGACTG GTGATTTCAG 8641 CGTGTCCTCT CCAAATGAAA TGAACTTCCT TATATAGAGG AAGGTCTTGC GAAGGATAGT 8701 GGGATTGTGC GTCATCCCTT ACGTCAGTGG AGATATCACA TCAATCCACT TGCTTTGAAG 8761 ACGTGGTTGG AACGTCTTCT TTTTCCACGA TGCTCCTCGT GGGTGGGGGT CCATCTTTGG 8821 GACCACTGTC GGCAGAGGCA TCTTGAACGA TAGCCTTTCC TTTATCGCAA TGATGGCATT 8881 TGTAGGTGCC ACCTTCCTTT TCTACTGTCC TTTTGATGAA GTGACAGATA GCTGGGCAAT 8941 GGAATCCGAG GAGGTTTCCC GATATTACCC TTTGTTGAAA AGTCTCAATA GCCCTTTGGT 9001 CTTCTGAGAC TGTATCTTTG ATATTCTTGG AGTAGACGAG AGTGTCGTGC TCCACCATGT 9061 TATCACATCA ATCCACTTGC TTTGAAGACG TGGTTGGAAC GTCTTCTTTT TCCACGATGC 9121 TCCTCGTGGG TGGGGGTCCA TCTTTGGGAC CACTGTCGGC AGAGGCATCT TGAACGATAG 9181 CCTTTCCTTT ATCGCAATGA TGGCATTTGT AGGTGCCACC TTCCTTTTCT ACTGTCCTTT 9241 TGATGAAGTG ACAGATAGCT GGGCAATGGA ATCCGAGGAG GTTTCCCGAT ATTACCCTTT 9301 GTTGAAAAGT CTCAATAGCC CTTTGGTCTT CTGAGACTGT ATCTTTGATA TTCTTGGAGT 9361 AGACGAGAGT GTCGTGCTCC ACCATGTTGG CAAGCTGCTC TAGCCAATAC GCAAACCGCC 9421 TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA 9481 AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC 9541 TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA 9601 CACAGGAAAC AGCTATGACC ATGATTACG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.