pLV3-CMV-GLIS1-3×FLAG-EF1a-Cop

pLV3-CMV-GLIS1-3×FLAG-EF1a-CopGFP-Neo plasmid

PVT94697

pLV3-CMV-GLIS1-3×FLAG-EF1a-CopGFP-Neo plasmid Information

Plasmid name: pLV3-CMV-GLIS1(human)-3×FLAG-EF1a-CopGFP-Neo
Alias: NM_147193.4
Gene lenght: 1860bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-GLIS1-3×FLAG-EF1a-CopGFP-Neo plasmid Description

pLV3-CMV-GLIS1-3×FLAG-EF1a-CopGFP-Neo is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-GLIS1-3×FLAG-EF1a-CopGFP-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatggcaga ggcccgcaca tccctgtctg cccactgtcg 2341 gggcccgctg gccactggcc tgcacccaga cctggacctc ccgggccgaa gcctcgccac 2401 ccctgcgcct tcctgctacc ttctgggcag cgaacccagc tctggcctgg gcctccagcc 2461 cgagacccac ctccccgagg gcagcctgaa gcggtgctgc gtcttgggcc taccccccac 2521 ctccccagcc tcctcctcac cctgtgcctc ctccgacgtc acctccatca tccgctcctc 2581 ccagacgtct ctggtcacct gtgtaaatgg actccggagc ccccctctga cgggagatct 2641 ggggggccct tccaagcggg cccggcctgg ccctgcatcg acggacagcc atgagggcag 2701 cttgcaactt gaagcctgcc ggaaggcgag cttcctgaag caggaacccg cggatgagtt 2761 ttcagagctc tttgggcctc accagcaggg cctgccgccc ccctatcccc tgtctcagtt 2821 gccgcctggc ccaagccttg gaggcctggg gctgggcctg gcaggcaggg tggtggccgg 2881 gcggcaggcg tgccgctggg tggactgctg tgcagcctat gagcagcagg aggagctggt 2941 gcggcacatc gagaagagcc acatcgacca gcgcaagggc gaggacttca cctgcttctg 3001 ggctggctgc gtgcgccgct acaagccctt caacgcccgc tacaagctgc tcatccacat 3061 gcgagtgcac tcgggcgaga agcccaacaa gtgcatgttt gaaggctgca gcaaggcctt 3121 ctcacggctg gagaacctca agatccacct gaggagccac acgggcgaga agccgtacct 3181 gtgccagcac ccgggttgcc agaaggcctt cagcaactcc agcgaccgcg ccaagcacca 3241 gcgcacccac ctagacacga agccgtacgc ctgtcagatc cctggctgct ccaagcgcta 3301 cacagacccc agctccctcc gcaagcacgt caaggcccat tcagccaaag agcagcaggt 3361 gcgtaagaag ctgcatgcgg gccctgacac cgaggccgac gtcctgaccg agtgtctggt 3421 cctgcagcag ctccacacgt ccacacagct ggctgccagc gacggcaagg gtggctgtgg 3481 cctgggccag gagctgctcc caggtgtgta tcctggctcc atcacccccc ataacggact 3541 tgcatcgggc ctcctgcccc cagcgcacga cgtaccttcc aggcaccacc cgctggatgc 3601 caccaccagt tcccaccacc atctgtcccc tctgcccatg gctgagagca cccgggatgg 3661 gttggggccc ggcctcctct caccaatagt cagccccctg aaggggctgg ggccaccgcc 3721 gctgccccca tcctctcaga gccattctcc ggggggccag cccttcccca cactccccag 3781 caagccgtcc tacccaccct tccagagccc tccacccccg cctctgccca gcccacaagg 3841 ttaccagggc agtttccact ccatccagag ttgcttcccc tatggcgact gctaccggat 3901 ggctgaacca gcagccggtg gggacggact ggtcggggag acccacggtt tcaaccccct 3961 gcggcccaat ggctaccaca gcctcagcac gcccttgcct gccacaggct atgaggccct 4021 ggctgaggcc tcatgcccca cagcgctgcc acagcagcca tctgaagatg tggtgtccag 4081 cggccccgag gactgtggct tcttccccaa tggagccttt gaccactgcc tgggccacat 4141 cccctccatc tacacagaca ccggatccGA CTACAAAGAC CATGACGGTG ATTATAAAGA 4201 TCATGACATC GACTACAAGG ATGACGATGA CAAGTAgcgg ccgcGaagga tctgcgatcg 4261 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg 4321 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga 4381 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt 4441 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg 4501 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg 4561 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta 4621 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc 4681 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt 4741 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc 4801 caccatggag agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg 4861 caccctgaac ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg 4921 ccgcatgacc aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct 4981 gagccacgtg atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa 5041 ccccttcctg cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga 5101 ggacggcggc gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg 5161 cgacttcaag gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat 5221 catccgcagc aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg 5281 cagcttcgcc cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag 5341 ccacatgcac ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt 5401 cgccttccgc cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca 5461 gcacgccttc aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc 5521 cgtggacggc accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct 5581 aacatgcggt gacgtggagg agaatcccgg ccctGCCACC atggctgaac aagatggatt 5641 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 5701 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 5761 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct 5821 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 5881 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 5941 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 6001 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 6061 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 6121 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac 6181 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 6241 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 6301 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 6361 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaGTCGA 6421 CAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC 6481 TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG 6541 TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT 6601 GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC 6661 TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC 6721 TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT 6781 GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAATCA TCGTCCTTTC CTTGGCTGCT 6841 CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT 6901 CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT 6961 TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC ggtaccttta 7021 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 7081 ctggaagggc taattcactc ccaacgaaGa taagatctgc tttttgcttg tactgggtct 7141 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 7201 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 7261 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 7321 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 7381 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 7441 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 7501 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 7561 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 7621 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg 7681 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 7741 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 7801 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 7861 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 7921 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 7981 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 8041 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 8101 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 8161 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 8221 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 8281 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 8341 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 8401 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 8461 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 8521 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 8581 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 8641 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 8701 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 8761 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 8821 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 8881 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 8941 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 9001 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 9061 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 9121 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 9181 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 9241 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 9301 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 9361 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 9421 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 9481 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 9541 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 9601 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 9661 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 9721 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 9781 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 9841 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 9901 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 9961 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 10021 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 10081 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 10141 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 10201 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 10261 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca 10321 agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.