pU6-METTL16-sgRNA1-U6-METTL16-

pU6-METTL16-sgRNA1-U6-METTL16-sgRNA2-Cas9-Puro plasmid

PVT94683

pU6-METTL16-sgRNA1-U6-METTL16-sgRNA2-Cas9-Puro plasmid Information

Plasmid name: pU6-METTL16(human)-sgRNA1-U6-METTL16(human)-sgRNA2-Cas9-Puro
Alias: GTTGAGTCTCAAGGGAACTG/TATCATCCACTTCTGTTGCG
Gene lenght:
Host: Mammalian cells
Use(s): Gene editing
Fragment type: CRISPR
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pU6-METTL16-sgRNA1-U6-METTL16-sgRNA2-Cas9-Puro plasmid Description

pU6-METTL16-sgRNA1-U6-METTL16-sgRNA2-Cas9-Puro is a plasmid suitable for gene editing in mammalian cells.

pU6-METTL16-sgRNA1-U6-METTL16-sgRNA2-Cas9-Puro plasmid Sequence

1 GGCCTTTTGC TCACATGCGC CATTCTCGAG CATGCGCCAT TCTCGAGCAT GCTCGAGAAT 61 GGCGCATGTG AGGGCCTATT TCCCATGATT CCTTCATATT TGCATATACG ATACAAGGCT 121 GTTAGAGAGA TAATTGGAAT TAATTTGACT GTAAACACAA AGATATTAGT ACAAAATACG 181 TGACGTAGAA AGTAATAATT TCTTGGGTAG TTTGCAGTTT TAAAATTATG TTTTAAAATG 241 GACTATCATA TGCTTACCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG 301 TGGAAAGGAC GAAACACCGG GTTGAGTCTC AAGGGAACTG GTTTTAGAGC TAGAAATAGC 361 AAGTTAAAAT AAGGCTAGTC CGTTATCAAC TTGAAAAAGT GGCACCGAGT CGGTGCTTTT 421 TTGTTTTAGA GCTAGAAATA GCAAGTTAAA ATAAGGCTAG TCCGTTTTTA GCGCGTGCGC 481 CAATTCTGCA GACAAATGGC TCTAGAGAGG GCCTATTTCC CATGATTCCT TCATATTTGC 541 ATATACGATA CAAGGCTGTT AGAGAGATAA TTGGAATTAA TTTGACTGTA AACACAAAGA 601 TATTAGTACA AAATACGTGA CGTAGAAAGT AATAATTTCT TGGGTAGTTT GCAGTTTTAA 661 AATTATGTTT TAAAATGGAC TATCATATGC TTACCGTAAC TTGAAAGTAT TTCGATTTCT 721 TGGCTTTATA TATCTTGTGG AAAGGACGAA ACACCGTATC ATCCACTTCT GTTGCGGTTT 781 TAGAGCTAGA AATAGCAAGT TAAAATAAGG CTAGTCCGTT ATCAACTTGA AAAAGTGGCA 841 CCGAGTCGGT GCTTTTTTGT TTTAGAGCTA GAAATAGCAA GTTAAAATAA GGCTAGTCCG 901 TTTTTAGCGC GTGCGCCAAT TCTGCAGACA AATGGGGTAC CCGTTACATA ACTTACGGTA 961 AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT TGACGTCAAT AGTAACGCCA 1021 ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA 1081 GTACATCAAG TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG 1141 CCCGCCTGGC ATTGTGCCCA GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC 1201 TACGTATTAG TCATCGCTAT TACCATGGTC GAGGTGAGCC CCACGTTCTG CTTCACTCTC 1261 CCCATCTCCC CCCCCTCCCC ACCCCCAATT TTGTATTTAT TTATTTTTTA ATTATTTTGT 1321 GCAGCGATGG GGGCGGGGGG GGGGGGGGGG CGCGCGCCAG GCGGGGCGGG GCGGGGCGAG 1381 GGGCGGGGCG GGGCGAGGCG GAGAGGTGCG GCGGCAGCCA ATCAGAGCGG CGCGCTCCGA 1441 AAGTTTCCTT TTATGGCGAG GCGGCGGCGG CGGCGGCCCT ATAAAAAGCG AAGCGCGCGG 1501 CGGGCGGGAG TCGCTGCGCG CTGCCTTCGC CCCGTGCCCC GCTCCGCCGC CGCCTCGCGC 1561 CGCCCGCCCC GGCTCTGACT GACCGCGTTA CTCCCACAGG TGAGCGGGCG GGACGGCCCT 1621 TCTCCTCCGG GCTGTAATTA GCTGAGCAAG AGGTAAGGGT TTAAGGGATG GTTGGTTGGT 1681 GGGGTATTAA TGTTTAATTA CCTGGAGCAC CTGCCTGAAA TCACTTTTTT TCAGGTTGGA 1741 CCGGTGCCAC CATGGACTAT AAGGACCACG ACGGAGACTA CAAGGATCAT GATATTGATT 1801 ACAAAGACGA TGACGATAAG ATGGCCCCAA AGAAGAAGCG GAAGGTCGGT ATCCACGGAG 1861 TCCCAGCAGC CGACAAGAAG TACAGCATCG GCCTGGACAT CGGCACCAAC TCTGTGGGCT 1921 GGGCCGTGAT CACCGACGAG TACAAGGTGC CCAGCAAGAA ATTCAAGGTG CTGGGCAACA 1981 CCGACCGGCA CAGCATCAAG AAGAACCTGA TCGGAGCCCT GCTGTTCGAC AGCGGCGAAA 2041 CAGCCGAGGC CACCCGGCTG AAGAGAACCG CCAGAAGAAG ATACACCAGA CGGAAGAACC 2101 GGATCTGCTA TCTGCAAGAG ATCTTCAGCA ACGAGATGGC CAAGGTGGAC GACAGCTTCT 2161 TCCACAGACT GGAAGAGTCC TTCCTGGTGG AAGAGGATAA GAAGCACGAG CGGCACCCCA 2221 TCTTCGGCAA CATCGTGGAC GAGGTGGCCT ACCACGAGAA GTACCCCACC ATCTACCACC 2281 TGAGAAAGAA ACTGGTGGAC AGCACCGACA AGGCCGACCT GCGGCTGATC TATCTGGCCC 2341 TGGCCCACAT GATCAAGTTC CGGGGCCACT TCCTGATCGA GGGCGACCTG AACCCCGACA 2401 ACAGCGACGT GGACAAGCTG TTCATCCAGC TGGTGCAGAC CTACAACCAG CTGTTCGAGG 2461 AAAACCCCAT CAACGCCAGC GGCGTGGACG CCAAGGCCAT CCTGTCTGCC AGACTGAGCA 2521 AGAGCAGACG GCTGGAAAAT CTGATCGCCC AGCTGCCCGG CGAGAAGAAG AATGGCCTGT 2581 TCGGAAACCT GATTGCCCTG AGCCTGGGCC TGACCCCCAA CTTCAAGAGC AACTTCGACC 2641 TGGCCGAGGA TGCCAAACTG CAGCTGAGCA AGGACACCTA CGACGACGAC CTGGACAACC 2701 TGCTGGCCCA GATCGGCGAC CAGTACGCCG ACCTGTTTCT GGCCGCCAAG AACCTGTCCG 2761 ACGCCATCCT GCTGAGCGAC ATCCTGAGAG TGAACACCGA GATCACCAAG GCCCCCCTGA 2821 GCGCCTCTAT GATCAAGAGA TACGACGAGC ACCACCAGGA CCTGACCCTG CTGAAAGCTC 2881 TCGTGCGGCA GCAGCTGCCT GAGAAGTACA AAGAGATTTT CTTCGACCAG AGCAAGAACG 2941 GCTACGCCGG CTACATTGAC GGCGGAGCCA GCCAGGAAGA GTTCTACAAG TTCATCAAGC 3001 CCATCCTGGA AAAGATGGAC GGCACCGAGG AACTGCTCGT GAAGCTGAAC AGAGAGGACC 3061 TGCTGCGGAA GCAGCGGACC TTCGACAACG GCAGCATCCC CCACCAGATC CACCTGGGAG 3121 AGCTGCACGC CATTCTGCGG CGGCAGGAAG ATTTTTACCC ATTCCTGAAG GACAACCGGG 3181 AAAAGATCGA GAAGATCCTG ACCTTCCGCA TCCCCTACTA CGTGGGCCCT CTGGCCAGGG 3241 GAAACAGCAG ATTCGCCTGG ATGACCAGAA AGAGCGAGGA AACCATCACC CCCTGGAACT 3301 TCGAGGAAGT GGTGGACAAG GGCGCTTCCG CCCAGAGCTT CATCGAGCGG ATGACCAACT 3361 TCGATAAGAA CCTGCCCAAC GAGAAGGTGC TGCCCAAGCA CAGCCTGCTG TACGAGTACT 3421 TCACCGTGTA TAACGAGCTG ACCAAAGTGA AATACGTGAC CGAGGGAATG AGAAAGCCCG 3481 CCTTCCTGAG CGGCGAGCAG AAAAAGGCCA TCGTGGACCT GCTGTTCAAG ACCAACCGGA 3541 AAGTGACCGT GAAGCAGCTG AAAGAGGACT ACTTCAAGAA AATCGAGTGC TTCGACTCCG 3601 TGGAAATCTC CGGCGTGGAA GATCGGTTCA ACGCCTCCCT GGGCACATAC CACGATCTGC 3661 TGAAAATTAT CAAGGACAAG GACTTCCTGG ACAATGAGGA AAACGAGGAC ATTCTGGAAG 3721 ATATCGTGCT GACCCTGACA CTGTTTGAGG ACAGAGAGAT GATCGAGGAA CGGCTGAAAA 3781 CCTATGCCCA CCTGTTCGAC GACAAAGTGA TGAAGCAGCT GAAGCGGCGG AGATACACCG 3841 GCTGGGGCAG GCTGAGCCGG AAGCTGATCA ACGGCATCCG GGACAAGCAG TCCGGCAAGA 3901 CAATCCTGGA TTTCCTGAAG TCCGACGGCT TCGCCAACAG AAACTTCATG CAGCTGATCC 3961 ACGACGACAG CCTGACCTTT AAAGAGGACA TCCAGAAAGC CCAGGTGTCC GGCCAGGGCG 4021 ATAGCCTGCA CGAGCACATT GCCAATCTGG CCGGCAGCCC CGCCATTAAG AAGGGCATCC 4081 TGCAGACAGT GAAGGTGGTG GACGAGCTCG TGAAAGTGAT GGGCCGGCAC AAGCCCGAGA 4141 ACATCGTGAT CGAAATGGCC AGAGAGAACC AGACCACCCA GAAGGGACAG AAGAACAGCC 4201 GCGAGAGAAT GAAGCGGATC GAAGAGGGCA TCAAAGAGCT GGGCAGCCAG ATCCTGAAAG 4261 AACACCCCGT GGAAAACACC CAGCTGCAGA ACGAGAAGCT GTACCTGTAC TACCTGCAGA 4321 ATGGGCGGGA TATGTACGTG GACCAGGAAC TGGACATCAA CCGGCTGTCC GACTACGATG 4381 TGGACCATAT CGTGCCTCAG AGCTTTCTGA AGGACGACTC CATCGACAAC AAGGTGCTGA 4441 CCAGAAGCGA CAAGAACCGG GGCAAGAGCG ACAACGTGCC CTCCGAAGAG GTCGTGAAGA 4501 AGATGAAGAA CTACTGGCGG CAGCTGCTGA ACGCCAAGCT GATTACCCAG AGAAAGTTCG 4561 ACAATCTGAC CAAGGCCGAG AGAGGCGGCC TGAGCGAACT GGATAAGGCC GGCTTCATCA 4621 AGAGACAGCT GGTGGAAACC CGGCAGATCA CAAAGCACGT GGCACAGATC CTGGACTCCC 4681 GGATGAACAC TAAGTACGAC GAGAATGACA AGCTGATCCG GGAAGTGAAA GTGATCACCC 4741 TGAAGTCCAA GCTGGTGTCC GATTTCCGGA AGGATTTCCA GTTTTACAAA GTGCGCGAGA 4801 TCAACAACTA CCACCACGCC CACGACGCCT ACCTGAACGC CGTCGTGGGA ACCGCCCTGA 4861 TCAAAAAGTA CCCTAAGCTG GAAAGCGAGT TCGTGTACGG CGACTACAAG GTGTACGACG 4921 TGCGGAAGAT GATCGCCAAG AGCGAGCAGG AAATCGGCAA GGCTACCGCC AAGTACTTCT 4981 TCTACAGCAA CATCATGAAC TTTTTCAAGA CCGAGATTAC CCTGGCCAAC GGCGAGATCC 5041 GGAAGCGGCC TCTGATCGAG ACAAACGGCG AAACCGGGGA GATCGTGTGG GATAAGGGCC 5101 GGGATTTTGC CACCGTGCGG AAAGTGCTGA GCATGCCCCA AGTGAATATC GTGAAAAAGA 5161 CCGAGGTGCA GACAGGCGGC TTCAGCAAAG AGTCTATCCT GCCCAAGAGG AACAGCGATA 5221 AGCTGATCGC CAGAAAGAAG GACTGGGACC CTAAGAAGTA CGGCGGCTTC GACAGCCCCA 5281 CCGTGGCCTA TTCTGTGCTG GTGGTGGCCA AAGTGGAAAA GGGCAAGTCC AAGAAACTGA 5341 AGAGTGTGAA AGAGCTGCTG GGGATCACCA TCATGGAAAG AAGCAGCTTC GAGAAGAATC 5401 CCATCGACTT TCTGGAAGCC AAGGGCTACA AAGAAGTGAA AAAGGACCTG ATCATCAAGC 5461 TGCCTAAGTA CTCCCTGTTC GAGCTGGAAA ACGGCCGGAA GAGAATGCTG GCCTCTGCCG 5521 GCGAACTGCA GAAGGGAAAC GAACTGGCCC TGCCCTCCAA ATATGTGAAC TTCCTGTACC 5581 TGGCCAGCCA CTATGAGAAG CTGAAGGGCT CCCCCGAGGA TAATGAGCAG AAACAGCTGT 5641 TTGTGGAACA GCACAAGCAC TACCTGGACG AGATCATCGA GCAGATCAGC GAGTTCTCCA 5701 AGAGAGTGAT CCTGGCCGAC GCTAATCTGG ACAAAGTGCT GTCCGCCTAC AACAAGCACC 5761 GGGATAAGCC CATCAGAGAG CAGGCCGAGA ATATCATCCA CCTGTTTACC CTGACCAATC 5821 TGGGAGCCCC TGCCGCCTTC AAGTACTTTG ACACCACCAT CGACCGGAAG AGGTACACCA 5881 GCACCAAAGA GGTGCTGGAC GCCACCCTGA TCCACCAGAG CATCACCGGC CTGTACGAGA 5941 CACGGATCGA CCTGTCTCAG CTGGGAGGCG ACAAAAGGCC GGCGGCCACG AAAAAGGCCG 6001 GCCAGGCAAA AAAGAAAAAG ggatctcgcg agggcagagg aagccttcta acatgcggtg 6061 acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc 6121 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca 6181 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct 6241 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg 6301 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc 6361 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc 6421 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg 6481 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc 6541 gcgccggggt gcccgccttc ctggagacat ccgcgccccg caacctcccc ttctacgagc 6601 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca 6661 tgacccgcaa gcccggtgcc tgaGAATTCC TAGAGCTCGC TGATCAGCCT CGACTGTGCC 6721 TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG 6781 TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG 6841 GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA 6901 GAATAGCAGG CATGCTGGGG AGCGGCCGCA GGAACCCCTA GTGATGGAGT TGGCCACTCC 6961 CTCTCTGCGC GCTCGCTCGC TCACTGAGGC CGGGCGACCA AAGGTCGCCC GACGCCCGGG 7021 CTTTGCCCGG GCGGCCTCAG TGAGCGAGCG AGCGCGCAGC TGCCTGCAGG GGCGCCTGAT 7081 GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATACGTC AAAGCAACCA 7141 TAGTACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 7201 ACCGCTACAC TTGCCAGCGC CTTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 7261 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 7321 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT TGGGTGATGG TTCACGTAGT 7381 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 7441 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACTCTA TCTCGGGCTA TTCTTTTGAT 7501 TTATAAGGGA TTTTGCCGAT TTCGGTCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 7561 TTTAACGCGA ATTTTAACAA AATATTAACG TTTACAATTT TATGGTGCAC TCTCAGTACA 7621 ATCTGCTCTG ATGCCGCATA GTTAAGCCAG CCCCGACACC CGCCAACACC CGCTGACGCG 7681 CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTccggg 7741 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagACG AAAGGGCCTC 7801 GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT 7861 GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA 7921 AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG 7981 AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC 8041 CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG 8101 GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT 8161 CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA 8221 TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT 8281 GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA 8341 GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA 8401 ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT 8461 CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC 8521 ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT 8581 CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT 8641 CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT 8701 GGAAGCCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT 8761 ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA 8821 GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG 8881 ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT 8941 CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA 9001 AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA 9061 AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT 9121 CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG 9181 TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC 9241 CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA 9301 CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC 9361 AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC 9421 GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA 9481 GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG 9541 TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA 9601 TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.