pLV3-CMV-HMGA1-3×FLAG-EF1a-Cop

pLV3-CMV-HMGA1-3×FLAG-EF1a-CopGFP-Neo plasmid

PVT94681

pLV3-CMV-HMGA1-3×FLAG-EF1a-CopGFP-Neo plasmid Information

Plasmid name: pLV3-CMV-HMGA1(human)-3×FLAG-EF1a-CopGFP-Neo
Alias: NM_145899.3;HMG-R; HMGA1A; HMGIY
Gene lenght: 321bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-HMGA1-3×FLAG-EF1a-CopGFP-Neo plasmid Description

pLV3-CMV-HMGA1-3×FLAG-EF1a-CopGFP-Neo is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-HMGA1-3×FLAG-EF1a-CopGFP-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatgagtga gtcgagctcg aagtccagcc agcccttggc 2341 ctccaagcag gaaaaggacg gcactgagaa gcggggccgg ggcaggccgc gcaagcagcc 2401 tccggtgagt cccgggacag cgctggtagg gagtcagaag gagcccagcg aagtgccaac 2461 acctaagaga cctcggggcc gaccaaaggg aagcaaaaac aagggtgctg ccaagacccg 2521 gaaaaccacc acaactccag gaaggaaacc aaggggcaga cccaaaaaac tggagaagga 2581 ggaagaggag ggcatctcgc aggagtcctc ggaggaggag cagggatccG ACTACAAAGA 2641 CCATGACGGT GATTATAAAG ATCATGACAT CGACTACAAG GATGACGATG ACAAGTAgcg 2701 gccgcGaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac 2761 agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg 2821 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg 2881 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc 2941 cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac 3001 ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc 3061 ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc 3121 ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct 3181 tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg 3241 accggcgcct acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga 3301 gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg 3361 agagggcacc cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct 3421 gaccttcagc ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac 3481 ctaccccagc ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa 3541 cacccgcatc gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta 3601 cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag 3661 cgtgatcttc accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat 3721 gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta 3781 ctacagcttc gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct 3841 gcagaacggg ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga 3901 gctgggcatc gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg 3961 cgctcagtcg tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg 4021 cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctGCCAC 4081 Catggctgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 4141 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 4201 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 4261 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 4321 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 4381 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 4441 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 4501 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 4561 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga 4621 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 4681 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 4741 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 4801 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 4861 tgacgagttc ttctgaGTCG ACAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC 4921 TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT 4981 GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT 5041 GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT 5101 GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG 5161 GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG 5221 CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAATC 5281 ATCGTCCTTT CCTTGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT 5341 CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC 5401 TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC 5461 CGCCTCCCCG Cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac 5521 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaG ataagatctg 5581 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc 5641 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg 5701 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg 5761 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca 5821 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa 5881 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 5941 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa 6001 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 6061 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 6121 gcctagactt ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt 6181 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 6241 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 6301 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 6361 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 6421 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 6481 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 6541 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 6601 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 6661 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 6721 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 6781 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 6841 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 6901 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 6961 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 7021 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 7081 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 7141 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 7201 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 7261 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 7321 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 7381 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 7441 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 7501 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 7561 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 7621 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 7681 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 7741 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 7801 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 7861 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 7921 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 7981 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 8041 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 8101 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 8161 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 8221 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 8281 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga 8341 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg 8401 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg 8461 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct 8521 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa 8581 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc 8641 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag 8701 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt 8761 gtaaaacgac ggccagtgcc aagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.