pDC316-CMV-mCherry-U6-FZR1-shR

pDC316-CMV-mCherry-U6-FZR1-shRNA3 plasmid

PVT94639

pDC316-CMV-mCherry-U6-FZR1-shRNA3 plasmid Information

Plasmid name: pDC316-CMV-mCherry-U6-FZR1(human)-shRNA3
Alias: TGAGGTTCTGGAACGTCTTTA
Gene lenght:
Host: Mammalian cells,Adenovirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pDC316-CMV-mCherry-U6-FZR1-shRNA3 plasmid Description

pDC316-CMV-mCherry-U6-FZR1-shRNA3 is a plasmid suitable for use in mammalian cells, and can also be used for adenovirus packaging.

pDC316-CMV-mCherry-U6-FZR1-shRNA3 plasmid Sequence

1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt 61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg 361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc 421 gggtcaaagt tggcgtttta ttattatagt cagntctaga gatatactga gtcattaggg 481 actttccaat gggttttgcc cagtacataa ggtcaatagg ggtgaatcaa caggaaagtc 541 ccattggagc caagtacact gagtcaatag ggactttcca ttgggttttg cccagtacaa 601 aaggtcaata gggggtgagt caatgggttt ttcccattat tggcacgtac ataaggtcaa 661 taggggtgag tcattgggtt tttccagcca tttaattaaa acgccatgta ctttcccacc 721 attgacgtca atgggctatt gaaactaatg caacgtgacc tttaaacggt actttcccat 781 agctgattaa tgggaaagta ccgttctcga gccaatacac gtcaatggga agtgaaaggg 841 cagccaaaac gtaacaccgc cccggttttc ccctggaaat tccatattgg cactcattct 901 attggctgag ctgcgttcta cgtgggtata agaggcgcga ccagcgtcgg taccgtcgca 961 gtcttcggtc tgaccaccgt agaacgcaga tcgaattcca ccatggtgag caagggcgag 1021 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc 1081 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc 1141 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg 1201 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac 1261 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac 1321 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag 1381 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg 1441 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc 1501 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac 1561 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc 1621 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac 1681 tccaccggcg gcatggacga gctgtacaag tagagatctc gagatccacc ggatctagat 1741 aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc 1801 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta 1861 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 1921 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtct 1981 gggcaggaag agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct 2041 gttagagaga taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg 2101 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg 2161 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttgggttt atatatcttg 2221 tggaaaggac gaaacacctg cagttttttg gatccgTGAG GTTCTGGAAC GTCTTTAttc 2281 aagagaTAAA GACGTTCCAG AACCTCATTT TTTgtcgact tcgagcaact tgtttattgc 2341 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 2401 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat 2461 cgtctagcat cgaagatcca ataacttcgt atagcataca ttatacgaag ttataagtag 2521 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 2581 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 2641 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 2701 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 2761 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 2821 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 2881 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 2941 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 3001 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 3061 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 3121 ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 3181 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 3241 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 3301 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 3361 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 3421 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 3481 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 3541 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 3601 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 3661 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 3721 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 3781 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 3841 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 3901 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 3961 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 4021 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 4081 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 4141 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 4201 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 4261 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga 4321 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 4381 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 4441 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 4501 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 4561 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 4621 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 4681 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 4741 cactctaggc aaaatagcac cctcccgctc cagaacaaca tacagcgctt cacagcggca 4801 gcctaacagt cagccttacc agtaaaaaag aaaacctatt aaaaaaacac cactcgacac 4861 ggcaccagct caatcagtca cagtgtaaaa aagggccaag tgcagagcga gtatatatag 4921 gactaaaaaa tgacgtaacg gttaaagtcc acaaaaaaca cccagaaaac cgcacgcgaa 4981 cctacgccca gaaacgaaag ccaaaaaacc cacaacttcc tcaaatcgtc acttccgttt 5041 tcccacgtta cgtaacttcc cattttaaga aaactacaat tcccaacaca tacaagttac 5101 tccgccctaa aacctacgtc acccgccccg ttcccacgcc ccgcgccacg tcacaaactc 5161 caccccctca ttatcatatt ggcttcaatc caaaataagg tata

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.