pCMV-JAK1-3×HA-Neo plasmid

pCMV-JAK1-3×HA-Neo plasmid

PVT91068

pCMV-JAK1-3×HA-Neo plasmid Information

Plasmid name: pCMV-JAK1(human)-3×HA-Neo
Alias: NM_002227.4;AIIDE; JAK1A; JAK1B; JTK3
Gene lenght: 3462bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-JAK1-3×HA-Neo plasmid Description

pCMV-JAK1-3×HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-JAK1-3×HA-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatgca gtatctaaat 721 ataaaagagg actgcaatgc catggctttc tgtgctaaaa tgaggagctc caagaagact 781 gaggtgaacc tggaggcccc tgagccaggg gtggaagtga tcttctatct gtcggacagg 841 gagcccctcc ggctgggcag tggagagtac acagcagagg aactgtgcat cagggctgca 901 caggcatgcc gtatctctcc tctttgtcac aacctctttg ccctgtatga cgagaacacc 961 aagctctggt atgctccaaa tcgcaccatc accgttgatg acaagatgtc cctccggctc 1021 cactaccgga tgaggttcta tttcaccaat tggcatggaa ccaacgacaa tgagcagtca 1081 gtgtggcgtc attctccaaa gaagcagaaa aatggctacg agaaaaaaaa gattccagat 1141 gcaacccctc tccttgatgc cagctcactg gagtatctgt ttgctcaggg acagtatgat 1201 ttggtgaaat gcctggctcc tattcgagac cccaagaccg agcaggatgg acatgatatt 1261 gagaacgagt gtctagggat ggctgtcctg gccatctcac actatgccat gatgaagaag 1321 atgcagttgc cagaactgcc caaggacatc agctacaagc gatatattcc agaaacattg 1381 aataagtcca tcagacagag gaaccttctc accaggatgc ggataaataa tgttttcaag 1441 gatttcctaa aggaatttaa caacaagacc atttgtgaca gcagcgtgtc cacgcatgac 1501 ctgaaggtga aatacttggc taccttggaa actttgacaa aacattacgg tgctgaaata 1561 tttgagactt ccatgttact gatttcatca gaaaatgaga tgaattggtt tcattcgaat 1621 gacggtggaa acgttctcta ctacgaagtg atggtgactg ggaatcttgg aatccagtgg 1681 aggcataaac caaatgttgt ttctgttgaa aaggaaaaaa ataaactgaa gcggaaaaaa 1741 ctggaaaata aacacaagaa ggatgaggag aaaaacaaga tccgggaaga gtggaacaat 1801 ttttcttact tccctgaaat cactcacatt gtaataaagg agtctgtggt cagcattaac 1861 aagcaggaca acaagaaaat ggaactgaag ctctcttccc acgaggaggc cttgtccttt 1921 gtgtccctgg tagatggcta cttccggctc acagcagatg cccatcatta cctctgcacc 1981 gacgtggccc ccccgttgat cgtccacaac atacagaatg gctgtcatgg tccaatctgt 2041 acagaatacg ccatcaataa attgcggcaa gaaggaagcg aggaggggat gtacgtgctg 2101 aggtggagct gcaccgactt tgacaacatc ctcatgaccg tcacctgctt tgagaagtct 2161 gagcaggtgc agggtgccca gaagcagttc aagaactttc agatcgaggt gcagaagggc 2221 cgctacagtc tgcacggttc ggaccgcagc ttccccagct tgggagacct catgagccac 2281 ctcaagaagc agatcctgcg cacggataac atcagcttca tgctaaaacg ctgctgccag 2341 cccaagcccc gagaaatctc caacctgctg gtggctacta agaaagccca ggagtggcag 2401 cccgtctacc ccatgagcca gctgagtttc gatcggatcc tcaagaagga tctggtgcag 2461 ggcgagcacc ttgggagagg cacgagaaca cacatctatt ctgggaccct gatggattac 2521 aaggatgacg aaggaacttc tgaagagaag aagataaaag tgatcctcaa agtcttagac 2581 cccagccaca gggatatttc cctggccttc ttcgaggcag ccagcatgat gagacaggtc 2641 tcccacaaac acatcgtgta cctctatggc gtctgtgtcc gcgacgtgga gaatatcatg 2701 gtggaagagt ttgtggaagg gggtcctctg gatctcttca tgcaccggaa aagcgatgtc 2761 cttaccacac catggaaatt caaagttgcc aaacagctgg ccagtgccct gagctacttg 2821 gaggataaag acctggtcca tggaaatgtg tgtactaaaa acctcctcct ggcccgtgag 2881 ggcatcgaca gtgagtgtgg cccattcatc aagctcagtg accccggcat ccccattacg 2941 gtgctgtcta ggcaagaatg cattgaacga atcccatgga ttgctcctga gtgtgttgag 3001 gactccaaga acctgagtgt ggctgctgac aagtggagct ttggaaccac gctctgggaa 3061 atctgctaca atggcgagat ccccttgaaa gacaagacgc tgattgagaa agagagattc 3121 tatgaaagcc ggtgcaggcc agtgacacca tcatgtaagg agctggctga cctcatgacc 3181 cgctgcatga actatgaccc caatcagagg cctttcttcc gagccatcat gagagacatt 3241 aataagcttg aagagcagaa tccagatatt gtttcagaaa aaaaaccagc aactgaagtg 3301 gaccccacac attttgaaaa gcgcttccta aagaggatcc gtgacttggg agagggccac 3361 tttgggaagg ttgagctctg caggtatgac cccgaagggg acaatacagg ggagcaggtg 3421 gctgttaaat ctctgaagcc tgagagtgga ggtaaccaca tagctgatct gaaaaaggaa 3481 atcgagatct taaggaacct ctatcatgag aacattgtga agtacaaagg aatctgcaca 3541 gaagacggag gaaatggtat taagctcatc atggaatttc tgccttcggg aagccttaag 3601 gaatatcttc caaagaataa gaacaaaata aacctcaaac agcagctaaa atatgccgtt 3661 cagatttgta aggggatgga ctatttgggt tctcggcaat acgttcaccg ggacttggca 3721 gcaagaaatg tccttgttga gagtgaacac caagtgaaaa ttggagactt cggtttaacc 3781 aaagcaattg aaaccgataa ggagtattac accgtcaagg atgaccggga cagccctgtg 3841 ttttggtatg ctccagaatg tttaatgcaa tctaaatttt atattgcctc tgacgtctgg 3901 tcttttggag tcactctgca tgagctgctg acttactgtg attcagattc tagtcccatg 3961 gctttgttcc tgaaaatgat aggcccaacc catggccaga tgacagtcac aagacttgtg 4021 aatacgttaa aagaaggaaa acgcctgccg tgcccaccta actgtccaga tgaggtttat 4081 caacttatga ggaaatgctg ggaattccaa ccatccaatc ggacaagctt tcagaacctt 4141 attgaaggat ttgaagcact tttaaaaCTC GAGTACCCAT ACGACGTACC TGATTATGCA 4201 ACTTATCCTT ATGATGTACC TGATTATGCT AGCTATCCTT ATGATGTCCC AGACTACGCA 4261 TAGTGAGGGC CCGATATCTC GACAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA 4321 CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT 4381 TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT 4441 TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG 4501 TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG 4561 GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC 4621 GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAGC 4681 TGACGTCCTT TCCATGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT 4741 TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG 4801 CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG 4861 CCGCCTCCCC GCCTGGAAAC GGGGGAGGCT AACTGAAACA CGGAAGGAGA CAATACCGGA 4921 AGGAACCCGC GCTATGACGG CAATAAAAAG ACAGAATAAA ACGCACGGGT GTTGGGTCGT 4981 TTGTTCATAA ACGCGGGGTT CGGTCCCAGG GCTGGCACTC TGTCGATACC CCACCGAGAC 5041 CCCATTGGGG CCAATACGCC CGCGTTTCTT CCTTTTCCCC ACCCCACCCC CCAAGTTCGG 5101 GTGAAGGCCC AGGGCTCGCA GCCAACGTCG GGGCGGCAGG CCCTGCCATA GCAGATCTGC 5161 GCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 5221 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 5281 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 5341 GGGGCATCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 5401 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 5461 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 5521 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGGG GATTTCGGCC TATTGGTTAA 5581 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG TGTGTCAGTT 5641 AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA 5701 TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG 5761 CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT 5821 AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC 5881 AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG 5941 AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA 6001 AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC 6061 GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC 6121 TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA 6181 CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC 6241 GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT 6301 GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA 6361 AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC 6421 ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT 6481 TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC 6541 CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG 6601 CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT 6661 GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT 6721 TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA 6781 GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGCG 6841 AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT 6901 TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC 6961 GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG 7021 GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT 7081 CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT 7141 CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC 7201 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT 7261 GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC 7321 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG 7381 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG 7441 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG 7501 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 7561 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC 7621 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 7681 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT 7741 CGGGAAGCGT GGCGCTTTCT CAATGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 7801 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 7861 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG 7921 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 7981 GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC 8041 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 8101 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 8161 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 8221 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 8281 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA 8341 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC 8401 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA 8461 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA 8521 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT 8581 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG 8641 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC 8701 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG 8761 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG 8821 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT 8881 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT 8941 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC 9001 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC 9061 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG 9121 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA 9181 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA 9241 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC 9301 CCCGAAAAGT GCCACCTGAC GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTCGAC 9361 TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT 9421 GTTGGAGGTC GCTGAGTAGT GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC 9481 CGACAATTGC ATGAAGAATC TGCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.