pLV3-EF1a-EGFP-GCH1-PGK-mCherr

pLV3-EF1a-EGFP-GCH1-PGK-mCherry-Neo plasmid

PVT94562

pLV3-EF1a-EGFP-GCH1-PGK-mCherry-Neo plasmid Information

Plasmid name: pLV3-EF1a-EGFP-GCH1(human)-PGK-mCherry-Neo
Alias: NM_000161.3;DYT14; DYT5; DYT5a; GCH; GTP-CH-1; GTPCH1;HPABH4B
Gene lenght: 753bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green,Red
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: pmscv-3
Copy number: High
Induction:

pLV3-EF1a-EGFP-GCH1-PGK-mCherry-Neo plasmid Description

pLV3-EF1a-EGFP-GCH1-PGK-mCherry-Neo is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-EF1a-EGFP-GCH1-PGK-mCherry-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca 1441 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg 1501 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa 1561 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa 1621 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt 1681 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag 1741 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtATCGGT 1801 taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca 1861 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt 1921 tatcgatact agtggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg 1981 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg 2041 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 2101 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 2161 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc 2221 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt 2281 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt 2341 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc 2401 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag 2461 ctgtgaccgg cgcctactct agagctagcg aattcgccac catggtgagc aagggcgagg 2521 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca 2581 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt 2641 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct 2701 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt 2761 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact 2821 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga 2881 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca 2941 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca 3001 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca 3061 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg 3121 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg 3181 ccgccgggat cactctcggc atggacgagc tgtacaaggg cggtggaggc agtggtggag 3241 gcggtagcat ggagaagggc cctgtgcggg caccggcgga gaagccgcgg ggcgccaggt 3301 gcagcaatgg gttccccgag cgggatccgc cgcggcccgg gcccagcagg ccggcggaga 3361 agcccccgcg gcccgaggcc aagagcgcgc agcccgcgga cggctggaag ggcgagcggc 3421 cccgcagcga ggaggataac gagctgaacc tccctaacct ggcagccgcc tactcgtcca 3481 tcctgagctc gctgggcgag aacccccagc ggcaagggct gctcaagacg ccctggaggg 3541 cggcctcggc catgcagttc ttcaccaagg gctaccagga gaccatctca gatgtcctaa 3601 acgatgctat atttgatgaa gatcatgatg agatggtgat tgtgaaggac atagacatgt 3661 tttccatgtg tgagcatcac ttggttccat ttgttggaaa ggtccatatt ggttatcttc 3721 ctaacaagca agtccttggc ctcagcaaac ttgcgaggat tgtagaaatc tatagtagaa 3781 gactacaagt tcaggagcgc cttacaaaac aaattgctgt agcaatcacg gaagccttgc 3841 ggcctgctgg agtcggggta gtggttgaag caacacacat gtgtatggta atgcgaggtg 3901 tacagaaaat gaacagcaaa actgtgacca gcacaatgtt gggtgtgttc cgggaggatc 3961 caaagactcg ggaagagttc ctgactctca ttaggagctg aggatccgcg gccgctgggt 4021 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac 4081 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca 4141 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa 4201 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct 4261 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg 4321 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg 4381 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg 4441 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc 4501 atctccgggc ctttcgacct gcagcccaag cttaccagcc accatggtga gcaagggcga 4561 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc 4621 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac 4681 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct 4741 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga 4801 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga 4861 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa 4921 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat 4981 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat 5041 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta 5101 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat 5161 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca 5221 ctccaccggc ggcatggacg agctgtacaa gggatctcgc gagggcagag gaagtcttct 5281 aacatgcggt gacgtggagg agaatcccgg ccctatggct gaacaagatg gattgcacgc 5341 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat 5401 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt 5461 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg 5521 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag 5581 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc 5641 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc 5701 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga 5761 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga 5821 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg 5881 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg 5941 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc 6001 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc 6061 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag tcgacaatca 6121 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 6181 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 6241 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 6301 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 6361 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 6421 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 6481 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg 6541 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 6601 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 6661 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac 6721 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg 6781 aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact gggtctctct 6841 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 6901 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 6961 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt 7021 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga 7081 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 7141 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 7201 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc 7261 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 7321 gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca gagacggccc 7381 aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 7441 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 7501 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 7561 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 7621 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 7681 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 7741 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 7801 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 7861 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 7921 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 7981 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 8041 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 8101 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 8161 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 8221 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 8281 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 8341 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 8401 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 8461 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 8521 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 8581 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 8641 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 8701 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 8761 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 8821 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 8881 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 8941 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 9001 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 9061 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 9121 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 9181 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 9241 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 9301 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 9361 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 9421 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 9481 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 9541 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 9601 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 9661 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 9721 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca 9781 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa 9841 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt 9901 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag 9961 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct 10021 g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.