pLV3-CMV-MCS-6×His-EF1a-mCherr

pLV3-CMV-MCS-6×His-EF1a-mCherry-Neo plasmid

PVT94536

pLV3-CMV-MCS-6×His-EF1a-mCherry-Neo plasmid Information

Plasmid name: pLV3-CMV-MCS-6×His-EF1a-mCherry-Neo
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MCS-6×His-EF1a-mCherry-Neo plasmid Description

pLV3-CMV-MCS-6×His-EF1a-mCherry-Neo is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-MCS-6×His-EF1a-mCherry-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgaat ttaaatcgga tccatgcatc atcaccatca ccatTAgcgg 2341 ccgcGaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 2401 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc 2461 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 2521 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 2581 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc 2641 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc 2701 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg 2761 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt 2821 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga 2881 ccggcgccta cgctagacgc caccatggtg agcaagggcg aggaggataa catggccatc 2941 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc 3001 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag 3061 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac 3121 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc 3181 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg 3241 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc 3301 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc 3361 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg 3421 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg 3481 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac 3541 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac 3601 gagctgtaca agggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag 3661 gagaatcccg gccctGCCAC Catggctgaa caagatggat tgcacgcagg ttctccggcc 3721 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 3781 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 3841 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg 3901 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 3961 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta 4021 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 4081 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 4141 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 4201 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg 4261 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 4321 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 4381 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 4441 atcgccttct atcgccttct tgacgagttc ttctgaGTCG ACAATCAACC TCTGGATTAC 4501 AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA 4561 TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC 4621 TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA 4681 CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC 4741 ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC 4801 ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC 4861 GTGGTGTTGT CGGGGAAATC ATCGTCCTTT CCTTGGCTGC TCGCCTGTGT TGCCACCTGG 4921 ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT 4981 TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG 5041 AGTCGGATCT CCCTTTGGGC CGCCTCCCCG Cggtaccttt aagaccaatg acttacaagg 5101 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact 5161 cccaacgaaG ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct 5221 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc 5281 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc 5341 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta 5401 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg 5461 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 5521 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc 5581 tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 5641 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta 5701 gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg 5761 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 5821 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 5881 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 5941 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 6001 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 6061 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 6121 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 6181 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 6241 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 6301 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 6361 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 6421 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 6481 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 6541 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 6601 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 6661 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 6721 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 6781 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 6841 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 6901 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 6961 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 7021 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 7081 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 7141 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 7201 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 7261 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 7321 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 7381 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 7441 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 7501 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 7561 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 7621 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 7681 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 7741 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 7801 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 7861 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 7921 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 7981 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 8041 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 8101 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt 8161 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 8221 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 8281 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 8341 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.