pLV2-CMV-HA-mCherry-MCS-Neo pl

pLV2-CMV-HA-mCherry-MCS-Neo plasmid

PVT94549

pLV2-CMV-HA-mCherry-MCS-Neo plasmid Information

Plasmid name: pLV2-CMV-HA-mCherry-MCS-Neo
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: mCherry-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-HA-mCherry-MCS-Neo plasmid Description

pLV2-CMV-HA-mCherry-MCS-Neo is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-CMV-HA-mCherry-MCS-Neo plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcGC CACCATGTAC 2821 CCATACGATG TTCCAGATTA CGCTatggtg agcaagggcg aggaggataa catggccatc 2881 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc 2941 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag 3001 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac 3061 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc 3121 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg 3181 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc 3241 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc 3301 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg 3361 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg 3421 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac 3481 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac 3541 gagctgtaca aggGaGGTGG AGGTAGTGGA GGAGGCGGAt cACTCGAGcc cgggatccac 3601 cggtactagt tctagagcgg ccgcggatcc gcccctctcc ctcccccccc cctaacgtta 3661 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca 3721 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca 3781 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg 3841 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc 3901 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata 3961 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag 4021 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc 4081 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt 4141 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat 4201 gataagcttg ccacaaccat ggctgaacaa gatggattgc acgcaggttc tccggccgct 4261 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc 4321 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc 4381 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc 4441 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg 4501 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc 4561 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac 4621 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat 4681 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc 4741 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg 4801 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg 4861 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc 4921 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc 4981 gccttctatc gccttcttga cgagttcttc tgaacgcgtc tggaacaatc aacctctgga 5041 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 5101 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 5161 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 5221 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 5281 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 5341 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 5401 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 5461 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 5521 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 5581 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac 5641 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc 5701 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta 5761 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga 5821 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac 5881 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca 5941 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc 6001 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg 6061 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga 6121 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc 6181 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga 6241 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg 6301 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 6361 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 6421 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 6481 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta 6541 gcgtttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt 6601 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 6661 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 6721 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 6781 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 6841 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 6901 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 6961 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 7021 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 7081 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 7141 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 7201 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 7261 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 7321 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 7381 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat 7441 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 7501 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 7561 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 7621 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 7681 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 7741 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 7801 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 7861 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 7921 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 7981 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 8041 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 8101 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 8161 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 8221 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 8281 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 8341 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 8401 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 8461 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 8521 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 8581 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 8641 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 8701 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact 8761 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 8821 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 8881 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat 8941 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg 9001 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.