pLV3-CMV-Runx1-3×FLAG-CopGFP-P

pLV3-CMV-Runx1-3×FLAG-CopGFP-Puro plasmid

PVT94525

pLV3-CMV-Runx1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Runx1(Rat)-3×FLAG-CopGFP-Puro
Alias: NM_017325.2;Aml1; CBF-alpha-2; Cbfa2; PEA2-alpha;PEBP2-alpha
Gene lenght: 1350bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Rat
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Runx1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Runx1-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Runx1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgc gtatccccgt agatgccagc 2341 acgagccgcc gcttcacgcc gccttccacc gcgctgagcc ccggcaagat gagcgaggcg 2401 ctgccgctgg gcgccccgga tggcggcgcc gccctggcca gcaagctgag gagcggcgac 2461 cgcagcatgg tggaggtact agctgaccac cccggcgagc tagtccgcac cgacagcccc 2521 aacttcctct gctccgtgct gcccactcac tggcgctgca acaagaccct gcccatcgct 2581 ttcaaggtgg tagcgctggg cgacgttccg gatggcactc tggtcaccgt catggcgggc 2641 aatgacgaaa actactcggc ggagctgaga aacgccaccg cggccatgaa gaaccaagtg 2701 gcgagattca acgacctcag gtttgtcggt cggagcggca gaggcaagag cttcactctg 2761 accatcaccg tctttacaaa ccccccacaa gttgccacct accatagagc catcaaaatc 2821 acagtggacg gcccccgaga accccgaaga catcggcaga aactagatga tcagaccaag 2881 cccgggagtt tgtccttttc cgagcggctc agtgaactgg agcagctgcg gcgcacggcc 2941 atgagggtca gcccgcatca cccagccccc acgcccaacc ctcgggcctc cttgaaccac 3001 tccactgcct ttaaccctca gcctcaaagt cagatgcagg atgccaggca gatccagccg 3061 tccccaccgt ggtcctatga ccagtcttac cagtacctgg ggtccatcac ctcttctgtg 3121 cacccggcga cacccatttc tcccggccga gccagtggca tgaccagcct ctcagcggaa 3181 ctttccagtc gactctcaac agccccggac ctgacagcct tcggcgaccc gcgccagttc 3241 cccacgctgc cgtccatctc cgacccgcgc atgcactacc caggcgcctt cacctactcg 3301 cctcctgtca catcgggcat cggcattggc atgtcagcca tgagctcgac ctctcgctac 3361 cacacctacc tgccgccgcc ctacccaggc tcctcgcagg cgcaggccgg gcccttccag 3421 accggctcgc cctcctacca cctgtactac ggcacctcgg caggctccta ccaattctcc 3481 atggtgggcg gcgagcgatc acccccgcgc atcctgccgc cctgcaccaa cgcgtccacc 3541 ggagccgcgc tgctcaaccc cagcctcccc agccagagcg acgtggtgga aaccgagggc 3601 agccatagca actcgcccac caacatgcca cccgcgcgcc tggaggaggc tgtctggcgg 3661 ccctacggat ccGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC 3721 AAGGATGACG ATGACAAGta gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag 3781 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga 3841 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc 3901 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt 3961 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct 4021 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg 4081 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc 4141 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc 4201 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg 4261 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac 4321 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg 4381 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag 4441 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc 4501 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc 4561 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg 4621 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg 4681 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc 4741 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc 4801 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag 4861 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg 4921 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc 4981 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc 5041 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg 5101 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 5161 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 5221 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 5281 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 5341 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 5401 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 5461 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 5521 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 5581 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 5641 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 5701 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 5761 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 5821 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 5881 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 5941 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 6001 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 6061 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaATCATCG 6121 TCCTTTCCTt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 6181 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 6241 cggcctcttc cgcgtctTcg ccttcgccct cagacgagtc ggatctccct ttggGccgcc 6301 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 6361 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaGat aagatctgct 6421 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 6481 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 6541 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 6601 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 6661 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata 6721 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 6781 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctaTCCC GCCCCTAACT 6841 CCGCCCAtcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 6901 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 6961 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata 7021 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 7081 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 7141 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 7201 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 7261 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 7321 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 7381 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 7441 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 7501 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 7561 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 7621 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 7681 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 7741 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 7801 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 7861 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 7921 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 7981 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 8041 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 8101 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 8161 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 8221 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 8281 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 8341 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 8401 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 8461 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 8521 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 8581 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 8641 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 8701 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 8761 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 8821 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 8881 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 8941 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 9001 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 9061 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 9121 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 9181 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc 9241 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 9301 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 9361 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca 9421 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc 9481 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 9541 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 9601 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.