pLV3-CMV-EHMT1-3×FLAG-CopGFP-P

pLV3-CMV-EHMT1-3×FLAG-CopGFP-Puro plasmid

PVT94515

pLV3-CMV-EHMT1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-EHMT1(human)-3×FLAG-CopGFP-Puro
Alias: NM_024757.5;EHMT1-IT1; Eu-HMTase1; EUHMTASE1; FP13812;GLP; GLP1; KLEFS1; KMT1D
Gene lenght: 3894bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-EHMT1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-EHMT1-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-EHMT1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg ccgccgccga tgccgaggca 2341 gttccggcga ggggggagcc tcagcaggat tgctgtgtga aaaccgagct gctgggagaa 2401 gagacaccta tggctgccga tgaaggctca gcagagaaac aggcaggaga ggcccacatg 2461 gctgcggacg gtgagaccaa tgggtcttgt gaaaacagcg atgccagcag tcatgcaaat 2521 gctgcaaagc acactcagga cagcgcaagg gtcaaccccc aggatggcac caacacacta 2581 actcggatag cggaaaatgg ggtttcagaa agagactcag aagcggcgaa gcaaaaccac 2641 gtcactgccg acgactttgt gcagacttct gtcatcggca gcaacggata catcttaaat 2701 aagccggccc tacaggcaca gcccttgagg actaccagca ctctggcctc ttcgctgcct 2761 ggccatgctg caaaaaccct tcctggaggg gctggcaaag gcaggactcc aagcgctttt 2821 ccccagacgc cagccgcccc accagccacc cttggggagg ggagtgctga cacagaggac 2881 aggaagctcc cggcccctgg cgccgacgtc aaggtccaca gggcacgcaa gaccatgccg 2941 aagtccgtcg tgggcctgca tgcagccagt aaagatccca gagaagttcg agaagctaga 3001 gatcataagg aaccaaaaga ggagatcaac aaaaacattt ctgactttgg acgacagcag 3061 cttttacccc ccttcccatc ccttcatcag tcgctacctc agaaccagtg ctacatggcc 3121 accacaaaat cacagacagc ttgcttgcct tttgttttag cagctgcagt atctcggaag 3181 aaaaaacgaa gaatgggaac ctatagcctg gttcctaaga aaaagaccaa agtattaaaa 3241 cagaggacgg tgattgagat gtttaagagc ataactcatt ccactgtggg ttccaagggg 3301 gagaaggacc tgggcgccag cagcctgcac gtgaatgggg agagcctgga gatggactcg 3361 gatgaggacg actcagagga gctcgaggag gacgacggcc atggtgcaga gcaggcggcc 3421 gcgttcccca cagaggacag caggacttcc aaggagagca tgtcggaggc tgatcgcgcc 3481 cagaagatgg acggggagtc cgaggaggag caggagtccg tggacaccgg ggaggaggag 3541 gaaggcggtg acgagtctga cctgagttcg gaatccagca ttaagaagaa atttctcaag 3601 aggaaaggaa agaccgacag tccctggatc aagccagcca ggaaaaggag gcggagaagt 3661 agaaagaagc ccagcggtgc cctcggttct gagtcgtata agtcatctgc aggaagcgct 3721 gagcagacgg caccaggaga cagcacaggg tacatggaag tttctctgga ctccctggat 3781 ctccgagtca aaggaattct gtcttcacaa gcagaagggt tggccaacgg tccagatgtg 3841 ctggagacag acggcctcca ggaagtgcct ctctgcagct gccggatgga aacaccgaag 3901 agtcgagaga tcaccacact ggccaacaac cagtgcatgg ctacagagag cgtggaccat 3961 gaattgggcc ggtgcacaaa cagcgtggtc aagtatgagc tgatgcgccc ctccaacaag 4021 gccccgctcc tcgtgctgtg tgaagaccac cggggccgca tggtgaagca ccagtgctgt 4081 cctggctgtg gctacttctg cacagcgggt aattttatgg agtgtcagcc cgagagcagc 4141 atctctcacc gtttccacaa agactgtgcc tctcgagtca ataacgccag ctattgtccc 4201 cactgtgggg aggagagctc caaggccaaa gaggtgacga tagctaaagc agacaccacc 4261 tcgaccgtga caccagtccc cgggcaggag aagggctcgg ccctggaggg cagggccgac 4321 accacaacgg gcagtgctgc cgggccacca ctctcggagg acgacaagct gcagggtgca 4381 gcctcccacg tgcccgaggg ctttgatcca acgggacctg ctgggcttgg gaggccaact 4441 cccggccttt cccagggacc agggaaggaa accttggaga gcgctctcat cgccctcgac 4501 tcggaaaaac ccaagaagct tcgcttccac ccaaagcagc tgtacttctc cgccaggcaa 4561 ggggagcttc agaaggtgct cctcatgctg gtggacggaa ttgaccccaa cttcaaaatg 4621 gagcaccaga ataagcgctc tccactgcac gccgcggcag aggctggaca cgtggacatc 4681 tgccacatgc tggttcaggc gggcgctaat attgacacct gctcagaaga ccagaggacc 4741 ccgttgatgg aagcagccga aaacaaccat ctggaagcag tgaagtacct catcaaggct 4801 ggggccctgg tggatcccaa ggacgcagag ggctctacgt gtttgcacct ggctgccaag 4861 aaaggccact acgaagtggt ccagtacctg ctttcaaatg gacagatgga cgtcaactgt 4921 caggatgacg gaggctggac acccatgatc tgggccacag agtacaagca cgtggacctc 4981 gtgaagctgc tgctgtccaa gggctctgac atcaacatcc gagacaacga ggagaacatt 5041 tgcctgcact gggcggcgtt ctccggctgc gtggacatag ccgagatcct gctggctgcc 5101 aagtgcgacc tccacgccgt gaacatccac ggagactcgc cactgcacat tgccgcccgg 5161 gagaaccgct acgactgtgt cgtcctcttt ctttctcggg attcagatgt caccttaaag 5221 aacaaggaag gagagacgcc cctgcagtgt gcgagcctca actctcaggt gtggagcgct 5281 ctgcagatga gcaaggctct gcaggactcg gcccccgaca ggcccagccc cgtggagagg 5341 atagtgagca gggacatcgc tcgaggctac gagcgcatcc ccatcccctg tgtcaacgcc 5401 gtggacagcg agccatgccc cagcaactac aagtacgtct ctcagaactg cgtgacgtcc 5461 cccatgaaca tcgacagaaa tatcactcat ctgcagtact gcgtgtgcat cgacgactgc 5521 tcctccagca actgcatgtg cggccagctc agcatgcgct gctggtacga caaggatggc 5581 cggctcctgc cagagttcaa catggcggag cctcccttga tcttcgaatg caaccacgcg 5641 tgctcctgct ggaggaactg ccgaaatcgc gtcgtacaga atggtctcag ggcaaggctg 5701 cagctctacc ggacgcggga catgggctgg ggcgtgcggt ccctgcagga catcccacca 5761 ggcacctttg tctgcgagta tgttggggag ctgatttcag actcagaagc cgacgttcga 5821 gaggaagatt cttacctctt tgatctcgac aataaggacg gggaggttta ctgcatcgac 5881 gcgcggttct acgggaacgt cagccggttc atcaaccacc actgcgagcc caacctggtg 5941 cccgtgcgcg tgttcatggc ccaccaggac ctgcggttcc cccggatcgc cttcttcagc 6001 acccgcctga tcgaggccgg cgagcagctc gggtttgact atggagagcg cttctgggac 6061 atcaaaggca agctcttcag ctgccgctgc ggctccccca agtgccggca ctcgagcgcg 6121 gccctggccc agcgtcaggc cagcgcggcc caggaggccc aggaggacgg cttgcccgac 6181 accagctccg cggctgccgc cgacccccta ggatccGACT ACAAAGACCA TGACGGTGAT 6241 TATAAAGATC ATGACATCGA CTACAAGGAT GACGATGACA AGtagcggcc gcGaaggatc 6301 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag 6361 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg 6421 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata 6481 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg 6541 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat 6601 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc 6661 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg 6721 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac 6781 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg 6841 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc 6901 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc 6961 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc 7021 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc 7081 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag 7141 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc 7201 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc 7261 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg 7321 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg 7381 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc 7441 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg 7501 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc 7561 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga 7621 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc 7681 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg 7741 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc 7801 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 7861 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 7921 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 7981 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 8041 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 8101 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 8161 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 8221 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac 8281 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga 8341 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc 8401 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa 8461 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc 8521 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc 8581 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc 8641 gtggtgttgt cggggaaATC ATCGTCCTTT CCTtggctgc tcgcctgtgt tgccacctgg 8701 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct 8761 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tTcgccttcg ccctcagacg 8821 agtcggatct ccctttggGc cgcctccccg cctggtacct ttaagaccaa tgacttacaa 8881 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca 8941 ctcccaacga aGataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat 9001 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 9061 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 9121 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat 9181 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat 9241 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 9301 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 9361 gctctagcta TCCCGCCCCT AACTCCGCCC Atcccgcccc taactccgcc cagttccgcc 9421 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 9481 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga 9541 gaccaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat 9601 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 9661 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 9721 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 9781 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 9841 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 9901 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 9961 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 10021 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 10081 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 10141 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 10201 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 10261 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 10321 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 10381 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 10441 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 10501 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 10561 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 10621 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 10681 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 10741 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 10801 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 10861 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 10921 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 10981 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 11041 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 11101 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 11161 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 11221 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 11281 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 11341 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 11401 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 11461 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 11521 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 11581 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 11641 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 11701 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 11761 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 11821 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 11881 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 11941 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 12001 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 12061 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 12121 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 12181 gctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.