pLV3-CMV-MCS-NFE2L2-6×His-EF1a

pLV3-CMV-MCS-NFE2L2-6×His-EF1a-mCherry-Neo plasmid

PVT94503

pLV3-CMV-MCS-NFE2L2-6×His-EF1a-mCherry-Neo plasmid Information

Plasmid name: pLV3-CMV-MCS-NFE2L2(human)-6×His-EF1a-mCherry-Neo
Alias: NM_006164.5;HEBP1; IMDDHH; Nrf-2; NRF2
Gene lenght: 1815bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MCS-NFE2L2-6×His-EF1a-mCherry-Neo plasmid Description

pLV3-CMV-MCS-NFE2L2-6×His-EF1a-mCherry-Neo is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-MCS-NFE2L2-6×His-EF1a-mCherry-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatgatgga cttggagctg ccgccgccgg gactcccgtc 2341 ccagcaggac atggatttga ttgacatact ttggaggcaa gatatagatc ttggagtaag 2401 tcgagaagta tttgacttca gtcagcgacg gaaagagtat gagctggaaa aacagaaaaa 2461 acttgaaaag gaaagacaag aacaactcca aaaggagcaa gagaaagcct ttttcgctca 2521 gttacaacta gatgaagaga caggtgaatt tctcccaatt cagccagccc agcacatcca 2581 gtcagaaacc agtggatctg ccaactactc ccaggttgcc cacattccca aatcagatgc 2641 tttgtacttt gatgactgca tgcagctttt ggcgcagaca ttcccgtttg tagatgacaa 2701 tgaggtttct tcggctacgt ttcagtcact tgttcctgat attcccggtc acatcgagag 2761 cccagtcttc attgctacta atcaggctca gtcacctgaa acttctgttg ctcaggtagc 2821 ccctgttgat ttagacggta tgcaacagga cattgagcaa gtttgggagg agctattatc 2881 cattcctgag ttacagtgtc ttaatattga aaatgacaag ctggttgaga ctaccatggt 2941 tccaagtcca gaagccaaac tgacagaagt tgacaattat catttttact catctatacc 3001 ctcaatggaa aaagaagtag gtaactgtag tccacatttt cttaatgctt ttgaggattc 3061 cttcagcagc atcctctcca cagaagaccc caaccagttg acagtgaact cattaaattc 3121 agatgccaca gtcaacacag attttggtga tgaattttat tctgctttca tagctgagcc 3181 cagtatcagc aacagcatgc cctcacctgc tactttaagc cattcactct ctgaacttct 3241 aaatgggccc attgatgttt ctgatctatc actttgcaaa gctttcaacc aaaaccaccc 3301 tgaaagcaca gcagaattca atgattctga ctccggcatt tcactaaaca caagtcccag 3361 tgtggcatca ccagaacact cagtggaatc ttccagctat ggagacacac tacttggcct 3421 cagtgattct gaagtggaag agctagatag tgcccctgga agtgtcaaac agaatggtcc 3481 taaaacacca gtacattctt ctggggatat ggtacaaccc ttgtcaccat ctcaggggca 3541 gagcactcac gtgcatgatg cccaatgtga gaacacacca gagaaagaat tgcctgtaag 3601 tcctggtcat cggaaaaccc cattcacaaa agacaaacat tcaagccgct tggaggctca 3661 tctcacaaga gatgaactta gggcaaaagc tctccatatc ccattccctg tagaaaaaat 3721 cattaacctc cctgttgttg acttcaacga aatgatgtcc aaagagcagt tcaatgaagc 3781 tcaacttgca ttaattcggg atatacgtag gaggggtaag aataaagtgg ctgctcagaa 3841 ttgcagaaaa agaaaactgg aaaatatagt agaactagag caagatttag atcatttgaa 3901 agatgaaaaa gaaaaattgc tcaaagaaaa aggagaaaat gacaaaagcc ttcacctact 3961 gaaaaaacaa ctcagcacct tatatctcga agttttcagc atgctacgtg atgaagatgg 4021 aaaaccttat tctcctagtg aatactccct gcagcaaaca agagatggca atgttttcct 4081 tgttcccaaa agtaagaagc cagatgttaa gaaaaaccat catcaccatc accattagcg 4141 gccgcGaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac 4201 agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg 4261 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg 4321 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc 4381 cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac 4441 ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc 4501 ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc 4561 ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct 4621 tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg 4681 accggcgcct acgctagacg ccaccatggt gagcaagggc gaggaggata acatggccat 4741 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt 4801 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa 4861 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta 4921 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt 4981 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt 5041 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac 5101 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc 5161 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct 5221 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt 5281 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga 5341 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga 5401 cgagctgtac aagggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga 5461 ggagaatccc ggccctGCCA CCatggctga acaagatgga ttgcacgcag gttctccggc 5521 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 5581 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 5641 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac 5701 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 5761 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 5821 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 5881 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 5941 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 6001 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 6061 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 6121 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 6181 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 6241 catcgccttc tatcgccttc ttgacgagtt cttctgaGTC GACAATCAAC CTCTGGATTA 6301 CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG 6361 ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC 6421 CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA 6481 ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC 6541 CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT 6601 CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC 6661 CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG CTCGCCTGTG TTGCCACCTG 6721 GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC 6781 TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC 6841 GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCggtacctt taagaccaat gacttacaag 6901 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac 6961 tcccaacgaa Gataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc 7021 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 7081 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 7141 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt 7201 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt 7261 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 7321 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 7381 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 7441 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 7501 agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat 7561 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 7621 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 7681 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 7741 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 7801 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 7861 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 7921 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 7981 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 8041 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 8101 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 8161 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 8221 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 8281 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 8341 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 8401 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 8461 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 8521 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 8581 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 8641 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 8701 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 8761 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 8821 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 8881 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 8941 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 9001 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 9061 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 9121 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 9181 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 9241 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 9301 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 9361 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 9421 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 9481 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 9541 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 9601 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 9661 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct 9721 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 9781 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 9841 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 9901 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag 9961 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc 10021 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc 10081 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag 10141 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.