pLV3-CMV-TM4SF1-3×FLAG-CopGFP-

pLV3-CMV-TM4SF1-3×FLAG-CopGFP-Puro plasmid

PVT94424

pLV3-CMV-TM4SF1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-TM4SF1(human)-3×FLAG-CopGFP-Puro
Alias: NM_014220.3;H-L6; L6; M3S1; TAAL6
Gene lenght: 606bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-TM4SF1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-TM4SF1-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-TM4SF1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCATGT GCTATGGGAA GTGTGCACGA 2341 TGCATCGGAC ATTCTCTGGT GGGGCTCGCC CTCCTGTGCA TCGCGGCTAA TATTTTGCTT 2401 TACTTTCCCA ATGGGGAAAC AAAGTATGCC TCCGAAAACC ACCTCAGCCG CTTCGTGTGG 2461 TTCTTTTCTG GCATCGTAGG AGGTGGCCTG CTGATGCTCC TGCCAGCATT TGTCTTCATT 2521 GGGCTGGAAC AGGATGACTG CTGTGGCTGC TGTGGCCATG AAAACTGTGG CAAACGATGT 2581 GCGATGCTTT CTTCTGTATT GGCTGCTCTC ATTGGAATTG CAGGATCTGG CTACTGTGTC 2641 ATTGTGGCAG CCCTTGGCTT AGCAGAAGGA CCACTATGTC TTGATTCCCT CGGCCAGTGG 2701 AACTACACCT TTGCCAGCAC TGAGGGCCAG TACCTTCTGG ATACCTCCAC ATGGTCCGAG 2761 TGCACTGAAC CCAAGCACAT TGTGGAATGG AATGTATCTC TGTTTTCTAT CCTCTTGGCT 2821 CTTGGTGGAA TTGAATTCAT CTTGTGTCTT ATTCAAGTAA TAAATGGAGT GCTTGGAGGC 2881 ATATGTGGCT TTTGCTGCTC TCACCAACAG CAATATGACT GCggatccGA CTACAAAGAC 2941 CATGACGGTG ATTATAAAGA TCATGACATC GACTACAAGG ATGACGATGA CAAGtagcgg 3001 ccgcGaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 3061 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc 3121 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 3181 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 3241 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc 3301 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc 3361 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg 3421 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt 3481 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga 3541 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag 3601 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga 3661 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg 3721 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc 3781 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac 3841 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac 3901 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc 3961 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg 4021 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac 4081 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg 4141 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag 4201 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc 4261 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc 4321 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc 4381 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc 4441 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac 4501 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 4561 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 4621 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 4681 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 4741 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 4801 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 4861 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 4921 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa 4981 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 5041 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 5101 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 5161 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 5221 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 5281 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 5341 actgacaatt ccgtggtgtt gtcggggaaA TCATCGTCCT TTCCTtggct gctcgcctgt 5401 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 5461 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctTcgcctt 5521 cgccctcaga cgagtcggat ctccctttgg Gccgcctccc cgcctggtac ctttaagacc 5581 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga 5641 agggctaatt cactcccaac gaaGataaga tctgcttttt gcttgtactg ggtctctctg 5701 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 5761 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 5821 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 5881 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 5941 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 6001 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 6061 ttatcatgtc tggctctagc taTCCCGCCC CTAACTCCGC CCAtcccgcc cctaactccg 6121 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 6181 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 6241 gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta 6301 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 6361 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 6421 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 6481 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 6541 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 6601 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 6661 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 6721 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 6781 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 6841 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 6901 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 6961 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 7021 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 7081 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 7141 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 7201 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 7261 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 7321 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 7381 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 7441 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 7501 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 7561 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 7621 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 7681 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 7741 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 7801 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 7861 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 7921 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 7981 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 8041 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 8101 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 8161 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 8221 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 8281 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 8341 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 8401 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 8461 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 8521 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 8581 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 8641 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 8701 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg 8761 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc 8821 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac 8881 ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.