pLV3-CMV-ITPKA-FLuc-CopGFP-Pur

pLV3-CMV-ITPKA-FLuc-CopGFP-Puro plasmid

PVT90484

pLV3-CMV-ITPKA-FLuc-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-ITPKA(human-gc-opt)-FLuc-CopGFP-Puro
Alias: NM_002220.3;IP3-3KA; IP3KA
Gene lenght: 1383bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ITPKA-FLuc-CopGFP-Puro plasmid Description

pLV3-CMV-ITPKA-FLuc-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-ITPKA-FLuc-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc GCCACCatgA CTCTGCCCGG CGGACCTACA GGTATGGCTA GACCCGGCGG 2341 GGCAAGGCCT TGTAGCCCCG GTCTGGAAAG AGCGCCACGC AGATCCGTGG GTGAGCTCCG 2401 CCTGCTCTTT GAGGCACGGT GTGCGGCCGT TGCCGCTGCA GCAGCCGCTG GCGAACCAAG 2461 AGCCAGAGGC GCCAAACGAA GAGGGGGTCA GGTACCAAAC GGCCTGCCCA GAGCACCGCC 2521 TGCGCCAgtg atccctcagc tgaccgtgac agccgaggag cccgacgtgc ccccgaccag 2581 ccctgggccg ccggagcggg agagggactg cctcccggca gcgggctctt cgcacctgca 2641 gcagccgcgc cgcctttcca cctcgtcggt ctcctccact ggctcctcgt cgctgctcga 2701 ggactcggag gacgacctgc tgagcgacag tgagagccgg agccgcggca acgtgcagct 2761 ggaagcgggc gaggacgtgg gtcagaaaaa ccactggcag aagatccgga ccatggtcaa 2821 tctgccggtc ataagccctt tcaagaagcg ctacgcctgg gtgcagctgg cagggcacac 2881 tgggagtttt aaggcggcgg gcaccagcgg gctgatcctg aagcgctgct cggagccgga 2941 gcgctactgc ctggcgcggc tgatggctga cgcgctgcgc ggctgcgtgc ctgccttcca 3001 cggcgtggtg gagcgcgacg gcgaaagcta cctgcagctg caggacctgc tcgatggctt 3061 cgacggacct tgtgtgctcg actgcaaaat gggcgtcagg acttacctag aggaggagct 3121 gaccaaggcc cgtgagcggc ccaagctgcg gaaggacatg tacaagaaaa tgctggcggt 3181 ggatcctgaa gctcccacgg aggaggagca cgcgcagcgc gccgtcacca agccgcgcta 3241 catgcagtgg cgggaaggca tcagctccag caccaccctc ggcttccgca tcgagggcat 3301 caagaaagcg gacggctcct gcagcaccga cttcaagact acgcgaagcc gagagcaggt 3361 gcttcgcgtc tttgaagagt ttgtgcaagg agatgaggaa gtgctgaggc ggtatctgaa 3421 ccgcctgcag cagatccggg acaccctgga ggtatccgag ttcttcagga ggcacgaggt 3481 gatcggcagc tcgctcctct ttgtgcacga tcactgccat cgcgccggcg tgtggctcat 3541 cgacttcggc aagaccacgc ccctccccga tggccagatc ctggaccacc ggcggccctg 3601 ggaggagggc aaccgcgagg acggctattt gctggggctg gacaatctca ttggcatcct 3661 ggccagcctg gctgagagag aattcGCCAC Catggaagat gccaaaaaca ttaagaaggg 3721 cccagcgcca ttctacccac tcgaagacgg gaccgccggc gagcagctgc acaaagccat 3781 gaagcgctac gccctggtgc ccggcaccat cgcctttacc gacgcacata tcgaggtgga 3841 cattacctac gccgagtact tcgagatgag cgttcggctg gcagaagcta tgaagcgcta 3901 tgggctgaat acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc agttcttcat 3961 gcccgtgttg ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg acatctacaa 4021 cgagcgcgag ctgctgaaca gcatgggcat cagccagccc accgtcgtat tcgtgagcaa 4081 gaaagggctg caaaagatcc tcaacgtgca aaagaagcta ccgatcatac aaaagatcat 4141 catcatggat agcaagaccg actaccaggg cttccaaagc atgtacacct tcgtgacttc 4201 ccatttgcca cccggcttca acgagtacga cttcgtgccc gagagcttcg accgggacaa 4261 aaccatcgcc ctgatcatga acagtagtgg cagtaccgga ttgcccaagg gcgtagccct 4321 accgcaccgc accgcttgtg tccgattcag tcatgcccgc gaccccatct tcggcaacca 4381 gatcatcccc gacaccgcta tcctcagcgt ggtgccattt caccacggct tcggcatgtt 4441 caccacgctg ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga 4501 ggagctattc ttgcgcagct tgcaagacta taagattcaa tctgccctgc tggtgcccac 4561 actatttagc ttcttcgcta agagcactct catcgacaag tacgacctaa gcaacttgca 4621 cgagatcgcc agcggcgggg cgccgctcag caaggaggta ggtgaggccg tggccaaacg 4681 cttccaccta ccaggcatcc gccagggcta cggcctgaca gaaacaacca gcgccattct 4741 gatcaccccc gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga 4801 ggctaaggtg gtggacttgg acaccggtaa gacactgggt gtgaaccagc gcggcgagct 4861 gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc 4921 tctcatcgac aaggacggct ggctgcacag cggcgacatc gcctactggg acgaggacga 4981 gcacttcttc atcgtggacc ggctgaagag cctgatcaaa tacaagggct accaggtagc 5041 cccagccgaa ctggagagca tcctgctgca acaccccaac atcttcgacg ccggggtcgc 5101 cggcctgccc gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg 5161 taaaaccatg accgagaagg agatcgtgga ctatgtggcc agccaggtta caaccgccaa 5221 gaagctgcgc ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt 5281 ggacgcccgc aagatccgcg agattctcat taaggccaag aagggcggca agatcgccgt 5341 gTAggatccC TGCGATATCC AGCgcggccg cGaaggatct gcgatcgctc cggtgcccgt 5401 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat 5461 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg 5521 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac 5581 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc 5641 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt 5701 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag 5761 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg 5821 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt 5881 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc 5941 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc 6001 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac 6061 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg 6121 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac 6181 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg 6241 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg 6301 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac 6361 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc 6421 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc 6481 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc 6541 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag 6601 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc 6661 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac 6721 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc 6781 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg 6841 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc 6901 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg 6961 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg 7021 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg 7081 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac 7141 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc 7201 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg 7261 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg 7321 acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact 7381 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 7441 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg 7501 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg 7561 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg 7621 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc 7681 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg 7741 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc 7801 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct 7861 ctgcggcctc ttccgcgtct ccgccttcgc cctcagacga gtcggatctc cctttggccg 7921 cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac 7981 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaa ataagatctg 8041 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc 8101 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg 8161 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg 8221 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca 8281 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa 8341 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 8401 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa 8461 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 8521 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 8581 gcctagactt ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt 8641 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 8701 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 8761 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 8821 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 8881 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 8941 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 9001 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 9061 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 9121 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 9181 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 9241 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 9301 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 9361 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 9421 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 9481 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 9541 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 9601 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 9661 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 9721 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 9781 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 9841 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 9901 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 9961 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 10021 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 10081 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 10141 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 10201 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 10261 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 10321 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 10381 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 10441 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 10501 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 10561 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 10621 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 10681 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 10741 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga 10801 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg 10861 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg 10921 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct 10981 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa 11041 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc 11101 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag 11161 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt 11221 gtaaaacgac ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.