pLV3-CMV-H6pd-3×FLAG-ZsGreen1-

pLV3-CMV-H6pd-3×FLAG-ZsGreen1-Puro plasmid

PVT94416

pLV3-CMV-H6pd-3×FLAG-ZsGreen1-Puro plasmid Information

Plasmid name: pLV3-CMV-H6pd(mouse)-3×FLAG-ZsGreen1-Puro
Alias: NM_173371.4;G6pd1; Gpd-1; Gpd1
Gene lenght: 2391bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-H6pd-3×FLAG-ZsGreen1-Puro plasmid Description

pLV3-CMV-H6pd-3×FLAG-ZsGreen1-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-H6pd-3×FLAG-ZsGreen1-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca 1441 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg 1501 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa 1561 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa 1621 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt 1681 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag 1741 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc 1801 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata 1861 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aAATTTTCGG 1921 GTTTATTACA GGGACAGCAG AGATCCAGTT TATCGATAAG CTTGGGAGTT CCGCGTTACA 1981 TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA 2041 ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG 2101 GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTACG 2161 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC 2221 TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG 2281 ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA 2341 AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT 2401 CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 2461 GAGGTCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA TCGCCTGGAG ACGCCATCCA 2521 CGCTGTTTTG ACCTCCATAG AAGACACCGA CTCTACTAGA GGATCTATTT CCGGTGAATT 2581 CGCCACCatg aagcacacag gcatttggaa gatgctccta gcggcaatgt gcttggccct 2641 tctgggctgc ctacaggcgc aggaactcaa gggtcatgtt tccataatcc tgctgggagc 2701 aaccggggac ctagctaaaa agtacctgtg gcagggactg tttcagctat acctggatga 2761 ggctgggaag ggccacagtt tcagcttcca cggtgctgcc ctgacagccc cccagcaagg 2821 ccagaagctc atggacaagg tcttggaatc cctctcctgt cccaaggact tggtacccag 2881 tcgctgtgat gaactcaagg gtcagtttct acagctgagc caataccgcc agctgaagac 2941 agtggaggac tatcagaccc tcaacaagga tattgagacc caggtccagc aggatgggct 3001 ctgggaggca ggcaggatct tctacttcag tgtgccgccc tttgcctacg cagacattgc 3061 ccgtaacatc aacagcagct gccgaccaca cccaggtgcc tggctacggg ttgtttttga 3121 aaaacccttt ggccatgacc acctctcagc ccagcagctg gcttcagaac ttgggagctt 3181 tttccaggag gaggagatgt accgtgtgga tcattacctc ggcaagcagg cggtggctca 3241 gatcctgccc ttccgagatc agaaccgcaa ggccttggat ggcctctgga acaggcacca 3301 tgtggagcgg gtggagatca tcttgaagga gaccatagat gcggaaggtc gggccagttt 3361 ctatgaggag tatggcgtca tccgtgacac gctgcagaac cacctgactg agatcctcac 3421 gctggtggcc atggaactgc ctctcaacat cagcagctca gccgccgtgc tccagcataa 3481 gctctgggcc ttccaggccc tgcgggggct gcagaagagc agtgccatcc tgggccagta 3541 ccaggcctac agcggacagg tgcgccgaga gctgcagaag ccagacggct tccagagcct 3601 gacaccaacc ttcgcaggtg tccttgtcca catcgacaac ctgcgctggg agggtgtccc 3661 tttcatcctg atgtctggca aggccttgga tgagagagtg ggctatgttc ggattgtgtt 3721 taagaatcgg gcgtactgca cccagagcga gagacactgg gtgccggagc agagccggtg 3781 cctgccccag cagattatct tctacattgg acacggtgag ctgggccacc ctgccattct 3841 ggtcagccgg aacctgttca aaccctcact acccactcag aagtggaagg aggtgcagga 3901 ccagcctggg ctccgcctgt tcggccgtcc tctgtctgat tactacgcct acaggcctgt 3961 gcgggagcaa gacgcctact ccaccctcct gtcgcacatc ttccactgcc gaaaggagtc 4021 cttcatcacc acagagaacc tcttggcctc ctgggtcttc tggaccccgt tactggacag 4081 cctggccttt gaagttccac gcccataccc agggggagcc gagaatggcc agctgctgga 4141 ctttgagttt agcggtggtc agctgacctt ctcccagcag cagctggagg tgctgatacc 4201 agatctaggc tcagtcccaa agcccagtga cttccaggtc ctcggggccc ggtatagaca 4261 gagcccactg attaccgcct ggccagagga gctgatctct aagctggcca gtgacattga 4321 agccgccgcg gtgcaggcag tacggcactt cggcaagttc cacctggcac tgtcaggtgg 4381 ttcgagcccc atagctctct tccagcagct ggccacagga cattacagct tcccctgggc 4441 ccacacacac ctctggctgg ttgatgagcg ttgtgtccct ctctcagatc cagactccaa 4501 cttccagggc ctgcaggctc acctgctaca gcacgtcagg gtaccctact acaacatcca 4561 cccgatgcca gtgcacctgc accagcggct ctgtgccgag gaggaccagg gagctcagac 4621 ctatgccagt gagatctcag ccctggtggc caacagcagc ttcgacctgg tgctcctggg 4681 catgggcacc gacgggcaca cagcttctct gttcccacag tcccccacag gcctggatgg 4741 tgatcagctg gtggtgctga cggagagccc gttcaggcca caccaacgca tgagcctcag 4801 cctgcccctc atcaaccgag ccaagaaggt agctgtcctg gtcatgggca ggacaaaacg 4861 tgagatcaca acgttggtga gccgcgtggg ccatgaaccc aagaagtggc ccatctcagg 4921 tgtggtgcct ctttctgggc agctggtttg gtacatggat tacgaggcct ttctgggaGG 4981 ATCCTCGGTA CCAAGCTTAA GTGACTACAA GGATGACGAT GACAAGGATT ACAAAGACGA 5041 CGATGATAAG GACTATAAGG ATGATGACGA CAAATAAAGA TCCATCGATA CTAGTAAGGA 5101 TCTGCGATCG CTCCGGTGCC CGTCAGTGGG CAGAGCGCAC ATCGCCCACA GTCCCCGAGA 5161 AGTTGGGGGG AGGGGTCGGC AATTGAACGG GTGCCTAGAG AAGGTGGCGC GGGGTAAACT 5221 GGGAAAGTGA TGTCGTGTAC TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA GAACCGTATA 5281 TAAGTGCAGT AGTCGCCGTG AACGTTCTTT TTCGCAACGG GTTTGCCGCC AGAACACAGC 5341 TGAAGCTTCG AGGGGCTCGC ATCTCTCCTT CACGCGCCCG CCGCCCTACC TGAGGCCGCC 5401 ATCCACGCCG GTTGAGTCGC GTTCTGCCGC CTCCCGCCTG TGGTGCCTCC TGAACTGCGT 5461 CCGCCGTCTA GGTAAGTTTA AAGCTCAGGT CGAGACCGGG CCTTTGTCCG GCGCTCCCTT 5521 GGAGCCTACC TAGACTCAGC CGGCTCTCCA CGCTTTGCCT GACCCTGCTT GCTCAACTCT 5581 ACGTCTTTGT TTCGTTTTCT GTTCTGCGCC GTTACAGATC CAAGCTGTGA CCGGCGCCTA 5641 CTCTAGCATG GCCCAGTCCA AGCACGGCCT GACCAAGGAG ATGACCATGA AGTACCGCAT 5701 GGAGGGCTGC GTGGACGGCC ACAAGTTCGT GATCACCGGC GAGGGCATCG GCTACCCCTT 5761 CAAGGGCAAG CAGGCCATCA ACCTGTGCGT GGTGGAGGGC GGCCCCTTGC CCTTCGCCGA 5821 GGACATCTTG TCCGCCGCCT TCATGTACGG CAACCGCGTG TTCACCGAGT ACCCCCAGGA 5881 CATCGTCGAC TACTTCAAGA ACTCCTGCCC CGCCGGCTAC ACCTGGGACC GCTCCTTCCT 5941 GTTCGAGGAC GGCGCCGTGT GCATCTGCAA CGCCGACATC ACCGTGAGCG TGGAGGAGAA 6001 CTGCATGTAC CACGAGTCCA AGTTCTACGG CGTGAACTTC CCCGCCGACG GCCCCGTGAT 6061 GAAGAAGATG ACCGACAACT GGGAGCCCTC CTGCGAGAAG ATCATCCCCG TGCCCAAGCA 6121 GGGCATCTTG AAGGGCGACG TGAGCATGTA CCTGCTGCTG AAGGACGGTG GCCGCTTGCG 6181 CTGCCAGTTC GACACCGTGT ACAAGGCCAA GTCCGTGCCC CGCAAGATGC CCGACTGGCA 6241 CTTCATCCAG CACAAGCTGA CCCGCGAGGA CCGCAGCGAC GCCAAGAACC AGAAGTGGCA 6301 CCTGACCGAG CACGCCATCG CCTCCGGCTC CGCCTTGCCC gcggccgctg agggcagagg 6361 aagtcttcta acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc 6421 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc 6481 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt 6541 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt 6601 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc 6661 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca 6721 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc 6781 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc 6841 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg 6901 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga 6961 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta 7021 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 7081 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc 7141 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca 7201 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac 7261 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact 7321 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc 7381 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg 7441 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc 7501 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt ctccgccttc gccctcagac 7561 gagtcggatc tccctttggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa 7621 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca 7681 ctcccaacga aaataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat 7741 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 7801 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 7861 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat 7921 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat 7981 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 8041 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 8101 gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 8161 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 8221 tagtgaggag gcttttttgg aggcctagac ttttgcagag acggcccaaa ttcgtaatca 8281 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 8341 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 8401 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 8461 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 8521 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 8581 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 8641 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 8701 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 8761 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 8821 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 8881 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 8941 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 9001 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 9061 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 9121 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 9181 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 9241 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 9301 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 9361 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 9421 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 9481 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 9541 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 9601 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 9661 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 9721 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 9781 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 9841 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 9901 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 9961 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 10021 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 10081 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 10141 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 10201 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 10261 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 10321 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 10381 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 10441 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt 10501 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg 10561 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg 10621 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga 10681 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg 10741 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 10801 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca 10861 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.