pcDNA3-myc-CUL3 plasmid

pcDNA3-myc-CUL3 plasmid

PVT94542

pcDNA3-myc-CUL3 plasmid Information

Plasmid name: pcDNA3-myc-CUL3
Alias: Plasmid #19893
Gene lenght: 2304bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pcDNA3-myc-CUL3 plasmid Description

pcDNA3-myc-CUL3 is a plasmid suitable for protein expression in mammalian cells.

pcDNA3-myc-CUL3 plasmid Sequence

1 GACATTGATT ATTGACTAGT TATTAATAGT AATCAATTAC GGGGTCATTA GTTCATAGCC 61 CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA 121 ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA 181 CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC 241 AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT 301 GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT 361 TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC 421 GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT 481 GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA 541 TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCTCTGG CTAACTAGAG 601 AACCCACTGC TTACTGGCTT ATCGAAATTA ATACGACTCA CTATAGGGAG ACCCAAGCTA 661 TGGAGCAAAA GCTCATCTCA GAGGAAGATC TGGAATTAGA GCAAAAGCTC ATCTCAGAGG 721 AAGATCTGGA ATTAGAGCAA AAGCTCATCT CAGAGGAAGA TCTGGAATTC AGGATCCTGA 781 TATCCGCGGT ACCGAGCACC ATGTCGAATC TGAGCAAAGG CACGGGCAGC CGGAAGGACA 841 CCAAGATGCG GATCCGGGCC TTTCCGATGA CCATGGATGA AAAATATGTA AACAGCATTT 901 GGGACCTTCT GAAAAATGCA ATTCAAGAAA TCCAGCGTAA GAATAACAGT GGTCTTAGTT 961 TTGAGGAGCT CTATAGAAAT GCATATACAA TGGTTTTGCA TAAACATGGA GAAAAGCTCT 1021 ACACTGGACT AAGAGAAGTT GTTACCGAAC ATCTCATAAA TAAGGTGCGA GAAGATGTAC 1081 TAAATTCATT GAATAACAAC TTTCTTCAAA CGCTAAATCA AGCTTGGAAT GATCATCAAA 1141 CAGCTATGGT GATGATTAGA GACATACTAA TGTACATGGA CCGTGTGTAT GTACAACAAA 1201 ATAATGTGGA GAACGTCTAC AATTTGGGAT TAATTATTTT TCGAGATCAA GTTGTACGTT 1261 ATGGGTGTAT TAGGGATCAT CTACGGCAAA CTCTATTGGA TATGATTGCA AGAGAGCGGA 1321 AAGGAGAAGT CGTAGACAGA GGCGCAATAA GAAATGCTTG CCAGATGTTA ATGATTTTAG 1381 GTCTCGAAGG AAGATCAGTC TGTGAAGAAG ATTTTGAGGC TCCTTTTTTG GAAATGTCTG 1441 CAGAATTTTT TCAGATGGAA AGCCAGAAAT TTTTAGCAGA AAATAGTGCT TCAGTATATA 1501 TAAAGAAAGT AGAAGCTAGA ATTAATGAAG AAATAGAACG AGTGATGCAC TGCCTTGACA 1561 AATCAACGGA AGAACCAATT GTAAAGGTGG TTGAAAGGGA ACTCATTTCC AAGCACATGA 1621 AGACTATAGT AGAAATGGAG AATTCTGGGC TAGTACATAT GTTGAAAAAT GGAAAGACAG 1681 AAGACCTTGG TTGCATGTAC AAGTTATTTA GTCGTGTGCC AAATGGTTTG AAAACAATGT 1741 GTGAGTGTAT GAGTTCCTAT TTGAGGGAGC AAGGTAAAGC TCTTGTTTCT GAAGAAGGAG 1801 AAGGAAAGAA TCCTGTTGAC TATATCCAGG GCTTATTGGA TCTGAAGAGT AGGTTCGATC 1861 GCTTCCTCCT GGAATCATTC AACAATGACC GTCTCTTTAA ACAAACTATT GCGGGTGACT 1921 TTGAGTATTT TCTCAACCTC AACTCCAGGT CTCCTGAATA CCTCTCATTA TTTATTGATG 1981 ATAAGCTGAA AAAGGGAGTC AAAGGGCTAA CAGAACAAGA AGTAGAAACA ATATTGGATA 2041 AAGCAATGGT CCTTTTTAGG TTTATGCAAG AAAAAGATGT ATTTGAACGT TATTATAAAC 2101 AACACTTGGC AAGGAGACTT CTCACAAATA AAAGTGTTTC TGATGACTCT GAAAAAAACA 2161 TGATATCTAA GTTAAAGACT GAATGTGGAT GTCAGTTCAC GTCAAAACTG GAAGGAATGT 2221 TTAGGGATAT GAGCATCTCA AACACAACGA TGGATGAATT CAGGCAACAT CTACAGGCAA 2281 CTGGTGTATC TTTAGGTGGT GTTGATCTTA CAGTCCGGGT GCTCACGACA GGATATTGGC 2341 CCACTCAGTC AGCCACACCA AAGTGCAACA TCCCACCAGC ACCAAGACAT GCTTTTGAGA 2401 TATTCAGAAG GTTCTACTTA GCCAAACACA GTGGTCGACA GCTCACACTC CAGCATCATA 2461 TGGGTTCTGC AGATCTCAAT GCCACATTTT ATGGACCAGT TAAAAAGGAA GATGGATCTG 2521 AAGTTGGTGT TGGAGGTGCA CAAGTAACTG GCTCTAATAC ACGGAAGCAC ATATTGCAAG 2581 TTTCCACTTT CCAGATGACC ATATTAATGC TCTTTAATAA TAGAGAAAAA TACACATTTG 2641 AGGAAATTCA GCAAGAGACA GATATCCCTG AAAGAGAGCT TGTTAGAGCC CTACAGTCCC 2701 TCGCCTGTGG TAAACCAACA CAGCGGGTTC TTACAAAAGA ACCCAAATCA AAGGAAATAG 2761 AAAATGGTCA TATATTTACA GTTAATGATC AATTCACATC CAAACTACAC AGAGTCAAGA 2821 TTCAAACAGT TGCTGCCAAA CAAGGTGAAT CCGACCCAGA GAGGAAAGAA ACAAGGCAGA 2881 AAGTAGACGA CGACAGAAAA CATGAGATAG AAGCTGCTAT AGTGCGGATA ATGAAATCTA 2941 GAAAGAAGAT GCAGCACAAT GTTCTAGTAG CGGAGGTAAC TCAGCAGTTG AAGGCGCGAT 3001 TCTTACCAAG TCCAGTTGTT ATTAAGAAAC GTATTGAAGG ACTTATTGAG AGAGAATATT 3061 TGGCACGAAC ACCTGAGGAT CGCAAAGTAT ACACATATGT AGCATAAAAT GCGTTCAGAA 3121 ATTTGATTTA TTCTTGGACT GTACTCTTCG CATGGACTGG GAAGTTCTTT TAAATCATTA 3181 AATATTAAGA CGACCATCTC TTCTATTAAA TTACAGTACA TGTTCTAGAC CATTGAGATC 3241 AAGCCTTTAC TCCCTTTGAG AGTTTCCAAC ATCAGTTGAT TGAGCTTCAG GCTTTACAAC 3301 GTTTATCCCT GTAGAGATCA TCTTTACAGT TCCTCGGGAA AATGTGAATG TGCTGCGTTT 3361 TGTTTTCTTT ACTGTATGAA AACAGGAAAA TAAAAGAGAA ATTTAGAAAA TACAGCTCAT 3421 TACAATAAAA TTGTTGGATT TCATTTCAAA AAAAAAAAAA AAAAAACTCG AGCATGCATC 3481 TAGAGGGCCC TATTCTATAG TGTCACCTAA ATGCTAGAGC TCGCTGATCA GCCTCGACTG 3541 TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG 3601 AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA 3661 GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG 3721 AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA 3781 CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 3841 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 3901 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 3961 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 4021 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 4081 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 4141 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA 4201 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG TGTGTCAGTT 4261 AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA 4321 TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG 4381 CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT 4441 AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC 4501 AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG 4561 AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA 4621 AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC 4681 GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC 4741 TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA 4801 CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC 4861 GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT 4921 GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA 4981 AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC 5041 ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT 5101 TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC 5161 CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG 5221 CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT 5281 GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT 5341 TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA 5401 GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA 5461 ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC 5521 TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC 5581 GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT 5641 TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT 5701 AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT 5761 AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC 5821 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA 5881 GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG 5941 TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG 6001 CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG 6061 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA 6121 AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG 6181 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG 6241 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC 6301 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG 6361 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT 6421 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC 6481 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC 6541 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG 6601 TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA 6661 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC 6721 GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT 6781 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT 6841 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT 6901 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC 6961 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC 7021 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA 7081 CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG 7141 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC 7201 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT 7261 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA 7321 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT 7381 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA 7441 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC 7501 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA 7561 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT 7621 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC 7681 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA 7741 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA 7801 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 7861 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 7921 CGAAAAGTGC CACCTGACGT CGACGGATCG GGAGATCTCC CGATCCCCTA TGGTGCACTC 7981 TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TCTGCTCCCT GCTTGTGTGT 8041 TGGAGGTCGC TGAGTAGTGC GCGAGCAAAA TTTAAGCTAC AACAAGGCAA GGCTTGACCG 8101 ACAATTGCAT GAAGAATCTG CTTAGGGTTA GGCGTTTTGC GCTGCTTCGC GATGTACGGG 8161 CCAGATATAC GCGTT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.