pLV3-CMV-TRIP10-3×FLAG-CopGFP-

pLV3-CMV-TRIP10-3×FLAG-CopGFP-Puro plasmid

PVT94292

pLV3-CMV-TRIP10-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-TRIP10(human)-3×FLAG-CopGFP-Puro
Alias: NM_001288962.2;CIP4; HSTP; STOT; STP; TRIP-10
Gene lenght: 1803bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: EF1a-R
Copy number: High
Induction:

pLV3-CMV-TRIP10-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-TRIP10-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-TRIP10-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg attggggcac tgagctgtgg 2341 gatcagttcg aggtgctcga gcgccacacg cagtgggggc tggacctgtt ggacagatat 2401 gtaaagttcg tgaaagaacg caccgaagtg gaacaggctt acgccaaaca actgcggagc 2461 ctggtgaaaa aatatctgcc caagagacct gccaaggatg atcctgagtc caaattcagc 2521 cagcaacagt ccttcgtaca gattctccag gaggtgaatg actttgcagg ccagcgggag 2581 ctggtggctg agaacctcag tgtccgtgta tgtcttgagc tgaccaagta ctcacaagag 2641 atgaaacagg agaggaagat gcacttccaa gaagggcggc gggcccagca gcagctggaa 2701 aatggcttta aacagctgga gaatagtaag cgtaaatttg agcgggactg ccgggaggca 2761 gagaaggcag cccagactgc tgaacggcta gaccaggata tcaacgccac caaggctgat 2821 gtggagaagg ccaagcagca agcccacctt cggagtcaca tggccgaaga aagcaaaaac 2881 gaatatgcgg ctcaactgca gcgcttcaac cgagaccaag cccacttcta tttttcacag 2941 atgccccaga tattcgataa gctccaagac atggatgaac gcagggccac ccgcctgggt 3001 gccgggtatg ggctcctgtc ggaggccgag ctggaggtgg tgcccataat agccaagtgc 3061 ttggagggca tgaaggtggc tgcaaatgct gtggatccca agaacgactc ccacgtcctt 3121 atagagctgc acaagtcagg ttttgcccgc ccgggcgacg tggaattcga ggacttcagc 3181 cagcccatga accgtgcacc ctccgacagc agtctgggca ccccctcgga tggacggcct 3241 gaactccgag gcccgggtcg cagccgcacc aagcgctggc cttttggcaa gaagaacaag 3301 cctcgccccc cacccctctc ccccctgggg ggccccgtac cctcggcatt gcctaacgga 3361 cccccgtccc cccgctccgg ccgtgacccc ttggccatac tgagcgagat cagtaagtcg 3421 gtcaaaccga ggctagcatc cttccgcagc cttcgaggca gccgtgggac agtggtgacc 3481 gaggatttta gccacttgcc cccagagcag cagcgaaaac ggcttcaaca gcagttggaa 3541 gaacgcagtc gtgaacttca gaaggaggtt gaccagaggg aagccctaaa gaaaatgaag 3601 gatgtctatg agaagacacc tcagatgggg gaccccgcca gcttggagcc ccagatcgct 3661 gaaaccctga gcaacattga acggctgaaa ttggaagtgc agaagtatga ggcgtggctg 3721 gcagaagctg aaagtcgagt ccttagcaac cggggagaca gcctgagccg gcacgcccgg 3781 cctcccgacc cccccgctag cgccccgcca gacagcagca gcaacagcgc atcacaggac 3841 accaaggaga gctctgaaga gcctccctca gaagagagcc aggacacccc catttacacg 3901 gagtttgatg aggatttcga ggaggaaccc acatccccca taggtcactg tgtggccatc 3961 taccactttg aagggtccag cgagggcact atctctatgg ccgagggtga agacctcagt 4021 cttatggaag aagacaaagg ggacggctgg acccgggtca ggcggaaaga gggaggcgag 4081 ggctacgtgc ccacctccta cctccgagtc acgctcaatg gatccGACTA CAAAGACCAT 4141 GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA Gtagcggccg 4201 cGaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc 4261 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg 4321 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa 4381 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga 4441 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga 4501 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga 4561 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg 4621 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct 4681 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg 4741 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc 4801 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag 4861 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc 4921 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac 4981 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc 5041 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag 5101 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg 5161 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc 5221 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac 5281 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag 5341 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg 5401 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct 5461 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag 5521 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag 5581 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 5641 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 5701 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 5761 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 5821 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 5881 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 5941 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 6001 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 6061 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 6121 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct 6181 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 6241 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 6301 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 6361 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 6421 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 6481 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 6541 gacaattccg tggtgttgtc ggggaaATCA TCGTCCTTTC CTtggctgct cgcctgtgtt 6601 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 6661 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct Tcgccttcgc 6721 cctcagacga gtcggatctc cctttggGcc gcctccccgc ctggtacctt taagaccaat 6781 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg 6841 gctaattcac tcccaacgaa Gataagatct gctttttgct tgtactgggt ctctctggtt 6901 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 6961 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 7021 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg 7081 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa 7141 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 7201 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 7261 tcatgtctgg ctctagctaT CCCGCCCCTA ACTCCGCCCA tcccgcccct aactccgccc 7321 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 7381 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac 7441 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc 7501 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 7561 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 7621 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 7681 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 7741 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 7801 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 7861 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 7921 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 7981 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 8041 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 8101 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 8161 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 8221 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 8281 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 8341 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 8401 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 8461 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 8521 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 8581 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 8641 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 8701 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 8761 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 8821 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 8881 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 8941 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 9001 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 9061 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 9121 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 9181 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 9241 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 9301 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 9361 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 9421 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 9481 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 9541 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 9601 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 9661 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct 9721 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 9781 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc 9841 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 9901 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga 9961 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 10021 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 10081 cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.