pJ23119-Bsg-3×FLAG-rrnBT1 plas

pJ23119-Bsg-3×FLAG-rrnBT1 plasmid

PVT96267

pJ23119-Bsg-3×FLAG-rrnBT1 plasmid Information

Plasmid name: pJ23119-Bsg(mouse)-3×FLAG-rrnBT1
Alias: NM_009768.2;CD147; EMMPRIN; HT-7
Gene lenght: 1167bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: J23119
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: M13F
Reverse primer:
Copy number: High
Induction:

pJ23119-Bsg-3×FLAG-rrnBT1 plasmid Description

pJ23119-Bsg-3×FLAG-rrnBT1 is a plasmid suitable for protein expression in Escherichia coli.

pJ23119-Bsg-3×FLAG-rrnBT1 plasmid Sequence

1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 121 cactcattag gcaccccagg cttgacagct agctcagtcc taggtataat actagttttc 181 acacaggaaa cagctatgac catgattacg ccaagcttat ggcggcggcg ctgctgctgg 241 cgctggcctt cacgctcttg agcggccaag gcgcctgcgc ggcggctggt ttcctcaagg 301 caccactgtc gcaggagcgg tgggcggggg gcagcgtggt cctgcactgt gaggctgtgg 361 gcagccccat ccccgagatc cagtggtggt ttgaagggaa tgctccaaac gacagctgct 421 cccagctctg ggatggtgcc cggctggacc gtgttcacat ccatgccgcc taccgtcagc 481 atgcagccag ttcgctctct gttgatgggc tcaccgcaga ggacacaggc acttacgagt 541 gccgggccag cagtgaccca gaccgcaacc accttactcg gccacccagg gtcaagtggg 601 tccgtgccca ggcgagcgtg gtggtccttg aaccgggcac catccaaacc tctgtccagg 661 aagtcaactc caaaacacag cttacctgct ctttgaacag cagtggcgtt gacatcgttg 721 gccaccgctg gatgagaggt ggcaaggtac tgcaggagga cactctgccc gacctgcata 781 cgaagtacat agtggacgca gatgaccgct ctggggaata ttcctgcatc ttccttcctg 841 agcctgtggg cagaagcgag atcaatgtgg aagggccacc caggatcaag gtcggaaaga 901 aatcagagca ttccagtgag ggagagcttg cgaaactggt ctgcaagtcc gatgcatcct 961 accctcctat tacagattgg ttctggttta agacctctga cactggggaa gaagaggcaa 1021 tcaccaatag cactgaagcc aatggcaagt atgtggtggt atccacgcct gagaagtcac 1081 agctgaccat cagcaacctt gacgtaaatg ttgaccctgg cacctacgtg tgtaatgcca 1141 ccaacgccca gggcactact cgggaaacca tctcactgcg tgtgcggagc cgcatggcag 1201 ccctctggcc cttcctaggc atcgtggctg aggtcctggt gttggttacc atcatcttta 1261 tctatgagaa gaggcggaag ccagaccaga ccctggacga ggatgaccct ggcgccgccc 1321 cactgaaggg cagtggaact cacatgaatg acaaggacaa gaatgtacgc cagaggaacg 1381 ccaccggatc cGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC ATCGACTACA 1441 AGGATGACGA TGACAAGtag gaattcactg gccgtcgttt tacaacgtcg tgactgggaa 1501 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt 1561 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa 1621 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg 1681 tgcactctca gtacaatctg ctctgatgcc gcatagttaa TTTTTCCAGG CATCAAATAA 1741 AACGAAAGTC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG 1801 CTCTCCTGAG TAGGACAAAT CCGCCGGGAG CGGATTTGAA CGTTGCGAAG CAACGGCCCG 1861 GAGGGTGGCG GGCAGGACGC CCGCCATAAA CTGCCAGGCA TCAAATTAAG CAGAAGGCCA 1921 TCCTGACGGA TGGCCTTTTT GCGTTTCTAC AAACTCTgcc agccccgaca cccgccaaca 1981 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg 2041 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga 2101 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct 2161 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 2221 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 2281 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 2341 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 2401 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 2461 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 2521 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac 2581 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 2641 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 2701 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 2761 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 2821 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 2881 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 2941 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 3001 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 3061 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 3121 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 3181 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 3241 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 3301 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 3361 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 3421 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 3481 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 3541 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 3601 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 3661 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 3721 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 3781 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 3841 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 3901 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 3961 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 4021 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 4081 gtgagcgagg aagcggaaga

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.