pCMV-Ninj1-mCMV-EGFP plasmid

pCMV-Ninj1-mCMV-EGFP plasmid

PVT94222

pCMV-Ninj1-mCMV-EGFP plasmid Information

Plasmid name: pCMV-Ninj1(mouse)-mCMV-EGFP
Alias: NM_013610.4
Gene lenght: 459bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Ninj1-mCMV-EGFP plasmid Description

pCMV-Ninj1-mCMV-EGFP is a plasmid suitable for protein expression in mammalian cells.

pCMV-Ninj1-mCMV-EGFP plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc GCTAGCGCTA 601 CCGGACTCAG ATCTCGAGCT CCGAATTCGC CACCatggag tcgggcactg aggagtatga 661 gctcaacggc gacctgcgcc cgggctcccc cggttccccc gacgccttgc caccccgctg 721 gggtttgcgg aaccggccca tcaatgtaaa ccattatgcc aacaagaaga gcgctgcgga 781 gagcatgctg gacatcgcgc tgctcatggc caacgcgtcg cagctgaagg ccgtggtgga 841 gcagggcaat gatttcgcct tcttcgtgcc ccttgtggtc ctcatctcta tctccctcgt 901 gctgcagata ggagtgggcg tgctgctcat cttcctggtc aagtatgacc tcaacaaccc 961 ggccaagcac gccaagctgg actttcttaa caacctggcc acgggactgg ttttcatcat 1021 cgtcgtggtc aacatcttca ttacggcctt cggggtccag aagcctgtaa tggacgtggc 1081 gccccggcag TAGGGATCCG GGCCCGTTTA AACCCGCTGA TCAGCCTCGA CTGTGCCTTC 1141 TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC 1201 CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG 1261 TCATTCTATT CTGGGGGGTG GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGACAA 1321 TAGCAGGCAT GCTGGGGATG CGGTGGGCTC TATGGTCTAG AGATATACTG AGTCATTAGG 1381 GACTTTCCAA TGGGTTTTGC CCAGTACATA AGGTCAATAG GGGTGAATCA ACAGGAAAGT 1441 CCCATTGGAG CCAAGTACAC TGAGTCAATA GGGACTTTCC ATTGGGTTTT GCCCAGTACA 1501 AAAGGTCAAT AGGGGGTGAG TCAATGGGTT TTTCCCATTA TTGGCACGTA CATAAGGTCA 1561 ATAGGGGTGA GTCATTGGGT TTTTCCAGCC AaTTTAATTA AAACGCCATG TACTTTCCCA 1621 CCATTGACGT CAATGGGCTA TTGAAACTAA TGCAACGTGA CCTTTAAACG GTACTTTCCC 1681 ATAGCTGATT AATGGGAAAG TACCGTTCTC GAGCCAATAC ACGTCAATGG GAAGTGAAAG 1741 GGCAGCCAAA ACGTAACACC GCCCCGGTTT TCCCCTGGAA ATTCCATATT GGCACGCATT 1801 CTATTGGCTG AGCTGCGTTC TACGTGGGTA TAAGAGGCGC GACCAGCGTC GGTACCGTCG 1861 CAGTCTTCGG TCTGACCACC GTAGAACGCA GATCaccggt cgccaccatg gtgagcaagg 1921 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 1981 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 2041 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc 2101 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct 2161 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 2221 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 2281 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 2341 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga 2401 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 2461 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc 2521 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 2581 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc ggccgcgact 2641 ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc 2701 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat 2761 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 2821 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa 2881 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt 2941 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag 3001 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg 3061 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat 3121 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc 3181 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga 3241 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac 3301 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc 3361 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac 3421 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa 3481 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 3541 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 3601 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 3661 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 3721 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 3781 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag 3841 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt 3901 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg 3961 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg 4021 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg 4081 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg 4141 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca 4201 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc 4261 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc 4321 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca 4381 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga 4441 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg 4501 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg 4561 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg 4621 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga 4681 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag 4741 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct 4801 catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat 4861 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg 4921 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc 4981 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag 5041 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc 5101 aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 5161 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 5221 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 5281 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 5341 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 5401 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 5461 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 5521 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 5581 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 5641 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 5701 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 5761 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 5821 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 5881 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 5941 taaccgtatt accgccatgc at

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.