pLV3-CMV-Olr1-3×FLAG-CopGFP-Pu

pLV3-CMV-Olr1-3×FLAG-CopGFP-Puro plasmid

PVT90597

pLV3-CMV-Olr1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Olr1(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_138648.2;LOX-1; Scare1; SR-EI;
Gene lenght: 1089bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Olr1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Olr1-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Olr1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga cttttgatga caagatgaag 2341 cctgcgaatg acgagcctga tcagaagtca tgtggcaaga agcctaaagg tctgcatttg 2401 ctttcttccc catggtggtt ccctgctgct atgactctgg tcatcctctg cctggtgttg 2461 tcagtgaccc ttattgtaca gtggacacaa ttacgccagg tatctgacct cttaaaacaa 2521 taccaagcga accttactca gcaggatcgt atcctggaag ggcagatgtt agcccagcag 2581 aaggcagaaa acacttcaca ggaatcaaag aaggaactga aaggaaagat agacaccctc 2641 acccagaagc tgaacgagaa atccaaagag caggaggagc ttctacagaa gaatcagaac 2701 ctccaagaag ccctgcaaag agctgcaaac tcttcagagg agtcccagag agaactcaag 2761 ggaaagatag acaccatcac ccggaagctg gacgagaaat ccaaagagca ggaggagctt 2821 ctgcagatga ttcagaacct ccaagaagcc ctgcagagag ctgcaaactc ttcagaggag 2881 tcccagagag aactcaaggg aaagatagac accctcacct tgaagctgaa cgagaaatcc 2941 aaagagcagg aggagcttct acagaagaat cagaacctcc aagaagccct gcaaagagct 3001 gcaaactttt caggtccttg tccacaagac tggctctggc ataaagaaaa ctgttacctc 3061 ttccatgggc cctttagctg ggaaaaaaac cggcagacct gccaatcttt gggtggccag 3121 ttactacaaa ttaatggtgc agatgatctg acattcatct tacaagcaat ttcccatacc 3181 acctccccgt tctggattgg attgcatcgg aagaagcctg gccaaccatg gctatgggag 3241 aatggaactc ctttgaattt tcaattcttt aagaccaggg gcgtttcttt acagctatat 3301 tcatcaggca actgtgcata ccttcaagac ggagctgtgt tcgctgaaaa ctgcattcta 3361 attgcattca gcatatgtca gaagaagaca aatcatttgc aaattggatc cGACTACAAA 3421 GACCATGACG GTGATTATAA AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGtag 3481 cggccgcGaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc 3541 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg 3601 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg 3661 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc 3721 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct 3781 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc 3841 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt 3901 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg 3961 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg 4021 tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg 4081 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc 4141 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc 4201 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc 4261 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc 4321 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc 4381 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac 4441 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc 4501 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc 4561 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc 4621 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc 4681 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc 4741 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct 4801 cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg 4861 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc 4921 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc 4981 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc 5041 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc 5101 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc 5161 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc 5221 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc 5281 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag 5341 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac 5401 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat 5461 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 5521 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 5581 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 5641 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 5701 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 5761 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 5821 ggcactgaca attccgtggt gttgtcgggg aaATCATCGT CCTTTCCTtg gctgctcgcc 5881 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 5941 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctTcgc 6001 cttcgccctc agacgagtcg gatctccctt tggGccgcct ccccgcctgg tacctttaag 6061 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact 6121 ggaagggcta attcactccc aacgaaGata agatctgctt tttgcttgta ctgggtctct 6181 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 6241 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 6301 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 6361 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 6421 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 6481 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 6541 atcttatcat gtctggctct agctaTCCCG CCCCTAACTC CGCCCAtccc gcccctaact 6601 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 6661 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 6721 ctagactttt gcagagacca aattcgtaat catgtcatag ctgtttcctg tgtgaaattg 6781 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 6841 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 6901 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 6961 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 7021 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 7081 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 7141 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 7201 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 7261 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 7321 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 7381 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 7441 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 7501 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 7561 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 7621 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 7681 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 7741 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 7801 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 7861 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 7921 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 7981 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 8041 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 8101 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 8161 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 8221 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 8281 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 8341 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 8401 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 8461 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 8521 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 8581 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 8641 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 8701 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 8761 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 8821 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 8881 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 8941 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga 9001 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg 9061 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa 9121 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca 9181 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg 9241 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg 9301 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac 9361 gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.