pCMV-mCherry-FKBP12-GPAT4(152-

pCMV-mCherry-FKBP12-GPAT4(152-208aa)-Neo plasmid

PVT94196

pCMV-mCherry-FKBP12-GPAT4(152-208aa)-Neo plasmid Information

Plasmid name: pCMV-mCherry-FKBP12(human)-GPAT4(human)(152-208aa)-Neo
Alias: NM_000801.5;FKBP-12; FKBP-1A; FKBP1; FKBP12; PKC12;PKCI2; PPIASE
Gene lenght: 324bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: MCHERRY-F
Reverse primer:
Copy number: High
Induction:

pCMV-mCherry-FKBP12-GPAT4(152-208aa)-Neo plasmid Description

pCMV-mCherry-FKBP12-GPAT4(152-208aa)-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-mCherry-FKBP12-GPAT4(152-208aa)-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 661 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag 721 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc 781 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc 841 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc 901 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag 961 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag 1021 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc 1081 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg 1141 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac 1201 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg 1261 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc 1321 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg 1381 tacaagggtg gaggcggatc aggaggcggt ggatctatgg gagtgcaggt ggaaaccatc 1441 tccccaggag acgggcgcac cttccccaag cgcggccaga cctgcgtggt gcactacacc 1501 gggatgcttg aagatggaaa gaaatttgat tcctcccggg acagaaacaa gccctttaag 1561 tttatgctag gcaagcagga ggtgatccga ggctgggaag aaggggttgc ccagatgagt 1621 gtgggtcaga gagccaaact gactatatct ccagattatg cctatggtgc cactgggcac 1681 ccaggcatca tcccaccaca tgccactctc gtcttcgatg tggagcttct aaaactggaa 1741 GACCCGACGC GCTCCGCCAA CTCTGGCGCC GGGGCTGGTG CCGGCGCAAT CCTTTCCCGA 1801 aacttccagt acatcagcct tcggctcacg gtcctgtggg ggttaggagt gctgattcgg 1861 tactgctttc tgctgccgct caggatagca ctggctttca cagggattag ccttctggtg 1921 gtgggcacaa ctgtggtggg atacttgcca aatgggaggt ttaaggagtt ctgagcggcc 1981 gcgactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa 2041 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt 2101 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 2161 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg 2221 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 2281 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg 2341 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact 2401 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac 2461 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 2521 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga 2581 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca 2641 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa 2701 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 2761 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg 2821 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc 2881 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 2941 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 3001 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 3061 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 3121 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag 3181 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 3241 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 3301 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 3361 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga 3421 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 3481 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 3541 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 3601 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 3661 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 3721 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 3781 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 3841 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 3901 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 3961 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat 4021 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta 4081 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 4141 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga 4201 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg 4261 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac 4321 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc 4381 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat 4441 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag 4501 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 4561 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 4621 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 4681 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 4741 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 4801 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 4861 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 4921 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 4981 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 5041 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 5101 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 5161 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 5221 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 5281 tgtggataac cgtattaccg ccatgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.