pLV3-CMV-Cebpb-3-3×FLAG-CopGFP

pLV3-CMV-Cebpb-3-3×FLAG-CopGFP-Puro plasmid

PVT94128

pLV3-CMV-Cebpb-3-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Cebpb(mouse)-3-3×FLAG-CopGFP-Puro
Alias: NM_001287738.1;C/EBPbeta; CRP2; IL-6DBP; LAP; LIP; NF-IL6;NF-M; Nfil6;
Gene lenght: 825bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Cebpb-3-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Cebpb-3-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Cebpb-3-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg aagtggccaa cttctactac 2341 gagcccgact gcctggccta cggggccaag gcggcccgcg ccgcgccgcg cgcccccgcc 2401 gccgagccgg ccattggcga gcacgagcgc gccatcgact tcagccccta cctggagccg 2461 ctcgcgcccg ccgcggactt cgccgcgccc gcgcccgcgc accacgactt cctctccgac 2521 ctcttcgccg acgactacgg cgccaagccg agcaagaagc cggccgacta cggttacgtg 2581 agcctcggcc gcgcgggcgc caaggccgcg ccgcccgcct gcttcccgcc gccgcctccc 2641 gccgcgctca aggcggagcc gggcttcgaa cccgcggact gcaagcgcgc ggacgacgcg 2701 cccgccatgg cggccggttt cccgttcgcc ctgcgcgcct acctgggcta ccaggcgacg 2761 ccgagcggca gcagcggcag cctgtccacg tcgtcgtcgt ccagcccgcc cggcacgccg 2821 agccccgccg acgccaaggc cgcgcccgcc gcctgcttcg cggggccgcc ggccgcgccc 2881 gccaaggcca aggccaagaa gacggtggac aagctgagcg acgagtacaa gatgcggcgc 2941 gagcgcaaca acatcgcggt gcgcaagagc cgcgacaagg ccaagatgcg caacctggag 3001 acgcagcaca aggtgctgga gctgacggcg gagaacgagc ggctgcagaa gaaggtggag 3061 cagctgtcgc gagagctcag caccctgcgg aacttgttca agcagctgcc cgagccgctg 3121 ctggcctcgg cgggccactg cggatccGAC TACAAAGACC ATGACGGTGA TTATAAAGAT 3181 CATGACATCG ACTACAAGGA TGACGATGAC AAGtagcggc cgcGaaggat ctgcgatcgc 3241 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga 3301 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat 3361 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta 3421 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga 3481 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg 3541 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag 3601 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct 3661 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt 3721 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc 3781 accatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc 3841 accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc 3901 cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg 3961 agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac 4021 cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag 4081 gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc 4141 gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc 4201 atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc 4261 agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc 4321 cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc 4381 gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag 4441 cacgccttca agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc 4501 gtggacggca ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta 4561 acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc 4621 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac 4681 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg 4741 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 4801 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 4861 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 4921 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 4981 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 5041 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 5101 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 5161 acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt 5221 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 5281 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 5341 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 5401 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 5461 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 5521 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 5581 tcggggaaAT CATCGTCCTT TCCTtggctg ctcgcctgtg ttgccacctg gattctgcgc 5641 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 5701 ctgctgccgg ctctgcggcc tcttccgcgt ctTcgccttc gccctcagac gagtcggatc 5761 tccctttggG ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt 5821 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 5881 aaGataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 5941 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 6001 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 6061 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 6121 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 6181 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 6241 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 6301 aTCCCGCCCC TAACTCCGCC CAtcccgccc ctaactccgc ccagttccgc ccattctccg 6361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 6421 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agaccaaatt 6481 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 6541 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 6601 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 6661 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 6721 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 6781 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 6841 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 6901 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 6961 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 7021 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 7081 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 7141 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 7201 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 7261 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 7321 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 7381 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 7441 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 7501 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 7561 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 7621 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 7681 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 7741 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 7801 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 7861 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 7921 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 7981 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 8041 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 8101 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 8161 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 8221 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 8281 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 8341 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 8401 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 8461 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 8521 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 8581 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 8641 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 8701 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 8761 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 8821 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt 8881 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 8941 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 9001 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt 9061 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.