pCMV-FLAG-HSPB1-EGFP-Neo-2 pla

pCMV-FLAG-HSPB1-EGFP-Neo-2 plasmid

PVT90557

pCMV-FLAG-HSPB1-EGFP-Neo-2 plasmid Information

Plasmid name: pCMV-FLAG-HSPB1(human)-EGFP-Neo-2
Alias: NM_001540.5;CMT2F; HEL-S-102; HMN2B; HMND3; HS.76067;Hsp25; HSP27; HSP28; SRP27
Gene lenght: 615bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-FLAG-HSPB1-EGFP-Neo-2 plasmid Description

pCMV-FLAG-HSPB1-EGFP-Neo-2 is a plasmid suitable for protein expression in mammalian cells.

pCMV-FLAG-HSPB1-EGFP-Neo-2 plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaagcc actgggatcc gccaccATGG ATTACAAGGA TGACGACGAT AAGATGACCG 961 AGCGCCGCGT CCCCTTCTCG CTCCTGCGGG GCCCCAGCTG GGACCCCTTC CGCGACTGGT 1021 ACCCGCATAG CCGCCTCTTC GACCAGGCCT TCGGGCTGCC CCGGCTGCCG GAGGAGTGGT 1081 CGCAGTGGTT AGGCGGCAGC AGCTGGCCAG GCTACGTGCG CCCCCTGCCC CCCGCCGCCA 1141 TCGAGAGCCC CGCAGTGGCC GCGCCCGCCT ACAGCCGCGC GCTCAGCCGG CAACTCAGCA 1201 GCGGGGTCTC GGAGATCCGG CACACTGCGG ACCGCTGGCG CGTGTCCCTG GATGTCAACC 1261 ACTTCGCCCC GGACGAGCTG ACGGTCAAGA CCAAGGATGG CGTGGTGGAG ATCACCGGCA 1321 AGCACGAGGA GCGGCAGGAC GAGCATGGCT ACATCTCCCG GTGCTTCACG CGGAAATACA 1381 CGCTGCCCCC CGGTGTGGAC CCCACCCAAG TTTCCTCCTC CCTGTCCCCT GAGGGCACAC 1441 TGACCGTGGA GGCCCCCATG CCCAAGCTAG CCACGCAGTC CAACGAGATC ACCATCCCAG 1501 TCACCTTCGA GTCGCGGGCC CAGCTTGGGG GCCCAGAAGC TGCAAAATCC GATGAGACTG 1561 CCGCCAAGgg ctcgagtggc ggtggaggaa gcggcggagg tggatctatg gtgagcaagg 1621 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 1681 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 1741 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc 1801 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct 1861 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 1921 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 1981 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 2041 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga 2101 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 2161 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc 2221 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 2281 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtagtct agagggcccg 2341 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc 2401 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa 2461 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg 2521 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg 2581 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc 2641 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 2701 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 2761 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 2821 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 2881 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 2941 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 3001 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 3061 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 3121 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 3181 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 3241 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 3301 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt 3361 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc 3421 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga 3481 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 3541 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 3601 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga 3661 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 3721 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 3781 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 3841 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 3901 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 3961 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga 4021 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 4081 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 4141 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 4201 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 4261 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca 4321 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 4381 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc 4441 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 4501 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 4561 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg 4621 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 4681 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 4741 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 4801 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 4861 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 4921 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 4981 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 5041 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 5101 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 5161 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 5221 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 5281 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 5341 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 5401 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 5461 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 5521 tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc 5581 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 5641 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 5701 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 5761 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 5821 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 5881 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 5941 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 6001 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 6061 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 6121 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 6181 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 6241 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 6301 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 6361 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 6421 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 6481 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 6541 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 6601 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 6661 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 6721 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.